Piezoelectric and surface plasmon resonance biosensors for
Bacillus atrophaeus spores

J 2013

Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL a Petr SKLÁDAL

Základní údaje

Originální název

Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores

Název česky

Piezoelektrické a SPR biosensory pro stanoveni spor Bacillus atrophaeus

Autoři

FARKA, Zdeněk (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí), Jan PŘIBYL (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

International Journal of Electrochemical Science, Belgrade, Serbia, ESG, 2013, 1452-3981

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Srbsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.956

Kód RIV

RIV/00216224:14310/13:00067588

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000316562800008

Klíčová slova česky

biosensor; spory; piezosensor

Klíčová slova anglicky

Bacillus atrophaeus spore; Biosensor; Immunosensor; Quartz Crystal Microbalance; Surface Plasmon Resonance

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2014 11:16, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, food processing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbial biosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.

Česky

Imunospecifické biosensory - imunosensory - pro detekci spor Bacillus atrophaeus, konstrukce na bázi piezosensorů a plasmonové resonance.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL a Petr SKLÁDAL. Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2013, roč. 8, č. 1, s. 100-112. ISSN 1452-3981.

@article{1079670,
   author = {Farka, Zdeněk and Kovář, David and Přibyl, Jan and Skládal, Petr},
   article_location = {Belgrade, Serbia},
   article_number = {1},
   keywords = {Bacillus atrophaeus spore; Biosensor; Immunosensor; Quartz Crystal Microbalance; Surface Plasmon Resonance},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores},
   url = {http://www.electrochemsci.org/papers/vol8/80100100.pdf},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1079670
AU  - Farka, Zdeněk - Kovář, David - Přibyl, Jan - Skládal, Petr
PY  - 2013
TI  - Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores
JF  - International Journal of Electrochemical Science
VL  - 8
IS  - 1
SP  - 100-112
EP  - 100-112
PB  - ESG
SN  - 14523981
KW  - Bacillus atrophaeus spore
KW  - Biosensor
KW  - Immunosensor
KW  - Quartz Crystal Microbalance
KW  - Surface Plasmon Resonance
UR  - http://www.electrochemsci.org/papers/vol8/80100100.pdf
L2  - http://www.electrochemsci.org/papers/vol8/80100100.pdf
N2  - Cheap, rapid and user friendly tools for detection of microorganisms were intensively discussed during last decades. The use of immunospecific biosensors seems advantageous for samples originating from clinical, environmental and agricultural area, food processing and military. The piezoelectric (quartz crystal microbalance, QCM) and optical (surface plasmon resonance, SPR) biosensors represented by QCM Analyzer and Biacore 3000 were compared for future development of real-time and label-free microbial biosensors. The spores of Bacillus atrophaeus were used as a non-pathogenic surrogate for B. anthracis. Seven commercially available antibodies were immobilised on the sensor surfaces to select the most promising one. Various immobilisation techniques were used. The total time of analysis did not exceed 30 min and the estimated limits of detection (LOD) were 10^6 CFU mL-1 and 10^5 CFU mL-1 in case of QCM and SPR, respectively.
ER  -

FARKA, Zdeněk, David KOVÁŘ, Jan PŘIBYL a Petr SKLÁDAL. Piezoelectric and surface plasmon resonance biosensors for Bacillus atrophaeus spores. \textit{International Journal of Electrochemical Science}. Belgrade, Serbia: ESG, 2013, roč.~8, č.~1, s.~100-112. ISSN~1452-3981.

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