| RÁJECKÝ, Michal, Antonín LOJEK a Milan ČÍŽ. Differentiating between intra- and extracellular chemiluminescence in diluted whole-blood samples. International Journal of Laboratory Hematology. MALDEN: WILEY-BLACKWELL, 2012, roč. 34, č. 2, s. 136-142. ISSN 1751-5521. Dostupné z: https://dx.doi.org/10.1111/j.1751-553X.2011.01370.x. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1080654,
author = {Rájecký, Michal and Lojek, Antonín and Číž, Milan},
article_location = {MALDEN},
article_number = {2},
doi = {http://dx.doi.org/10.1111/j.1751-553X.2011.01370.x},
keywords = {Chemiluminescence; isoluminol; luminol; phagocyte; whole blood},
language = {eng},
issn = {1751-5521},
journal = {International Journal of Laboratory Hematology},
title = {Differentiating between intra- and extracellular chemiluminescence in diluted whole-blood samples},
volume = {34},
year = {2012}
}
TY - JOUR
ID - 1080654
AU - Rájecký, Michal - Lojek, Antonín - Číž, Milan
PY - 2012
TI - Differentiating between intra- and extracellular chemiluminescence in diluted whole-blood samples
JF - International Journal of Laboratory Hematology
VL - 34
IS - 2
SP - 136-142
EP - 136-142
PB - WILEY-BLACKWELL
SN - 17515521
KW - Chemiluminescence
KW - isoluminol
KW - luminol
KW - phagocyte
KW - whole blood
N2 - Introduction: The differentiation between extra- and intracellular production of reactive oxygen species (ROS) in whole blood was measured by luminol- and isoluminol-enhanced chemiluminescence (CL). Methods: Azide (total CL inhibition), azide + horseradish peroxidase (HRP, restoring extracellular CL), superoxide dismutase + catalase (depleting extracellular ROS) and HRP (enhancing extracellular CL) were used to modulate luminol-and isoluminol-enhanced CL (10(-6)-10(-3) M luminophores) of 125x diluted whole blood which was activated by both calcium ionophore A23187 (Ca-I) and opsonized zymosan particles (OZP) separately. Results: Both activators stimulated intra-and extracellular production of ROS. Luminol-enhanced CL of Ca-I-activated samples detected the intracellular ROS, and with the addition of HRP detected the extracellular CL as well. CL enhanced with isoluminol in concentrations of 10(-4) M or less was mostly extracellular. There was a mixture of intra-and extracellular CL in OZP-activated samples, probably because of the ingestion of luminophore molecules. Conclusion: Measurement of Ca-I-activated CL enhanced with 10(-4) M luminol is recommended for the detection of intracellular ROS. The addition of HRP leads to the detection of overall ROS production while the OZP-activated system with its addition of HRP can only be used to detect overall ROS production. Ca-I-activated CL enhanced with 10(-4) M isoluminol and with addition of HRP is recommended for the detection of extracellular CL.
ER -
RÁJECKÝ, Michal, Antonín LOJEK a Milan ČÍŽ. Differentiating between intra- and extracellular chemiluminescence in diluted whole-blood samples. \textit{International Journal of Laboratory Hematology}. MALDEN: WILEY-BLACKWELL, 2012, roč.~34, č.~2, s.~136-142. ISSN~1751-5521. Dostupné z: https://dx.doi.org/10.1111/j.1751-553X.2011.01370.x.
|
