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@article{1082690,
author = {Bidmanová, Šárka and Hlaváček, Antonín and Damborský, Jiří and Prokop, Zbyněk},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.snb.2011.09.046},
keywords = {pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein},
language = {eng},
issn = {0925-4005},
journal = {SENSORS AND ACTUATORS B: CHEMICAL},
title = {Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing},
volume = {161},
year = {2012}
}
TY - JOUR
ID - 1082690
AU - Bidmanová, Šárka - Hlaváček, Antonín - Damborský, Jiří - Prokop, Zbyněk
PY - 2012
TI - Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing
JF - SENSORS AND ACTUATORS B: CHEMICAL
VL - 161
IS - 1
SP - 93-99
EP - 93-99
SN - 09254005
KW - pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein
N2 - An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol–gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0–9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring. Immobilized conjugates of 5(6)-carboxynaphthofluorescein and bovine serum albumin showed an enhanced sensitivity in alkaline pH levels, response for glutaraldehyde crosslinking ranged from pH 9.1 to 11.0 and from 8.3 to 10.0 for the ORMOCER entrapment, however, control of the indicator leaching was essential.
ER -
BIDMANOVÁ, Šárka, Antonín HLAVÁČEK, Jiří DAMBORSKÝ a Zbyněk PROKOP. Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing. \textit{SENSORS AND ACTUATORS B: CHEMICAL}. roč.~161, č.~1, s.~93-99. ISSN~0925-4005. doi:10.1016/j.snb.2011.09.046. 2012.
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