Upregulation of TNFa and IL-6 in both the ipsilateral and
contralateral dorsal root ganglia associated and non-associated
with unilateral nerve injury: A possible role for intrathecal
signaling

a 2012

Upregulation of TNFa and IL-6 in both the ipsilateral and contralateral dorsal root ganglia associated and non-associated with unilateral nerve injury: A possible role for intrathecal signaling

DUBOVÝ, Petr; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Václav BRÁZDA; Marek JOUKAL et al.

Základní údaje

Originální název

Upregulation of TNFa and IL-6 in both the ipsilateral and contralateral dorsal root ganglia associated and non-associated with unilateral nerve injury: A possible role for intrathecal signaling

Název česky

Zvýšená produkce TNFa a IL-6 v ipsilaterálních a kontralaterálních gangliích po jednostranném poškození periferního nervu. Možná role pro intratekální signalizaci.

Autoři

DUBOVÝ, Petr; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Václav BRÁZDA; Marek JOUKAL a Lucie STREJČKOVÁ

Vydání

FENS Forum of Neuroscience Barcelona, 2012

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

TNFa; IL-6; spinální ganglia; unilaterální nervové poškození, signalizační cesta neurozánětu

Klíčová slova anglicky

TNFa; IL-6; dorsal root ganglia; unilateral nerve injury; signaling pathway of neuroinflammation

Příznaky

Mezinárodní význam

Změněno: 22. 10. 2016 02:52, doc. MUDr. Marek Joukal, Ph.D.

Anotace

V originále

Following mechanical injury to a peripheral nerve, Wallerian degeneration is an essential stimulus for inducing the cellular and molecular changes that underlie neuropathic pain. Compelling data suggest that hyperalgesia, allodynia and ongoing pain due to peripheral nerve injury are associated with neuroinflammation in the dorsal root ganglia (DRG), which is characterized by an upregulation of pro- and anti-inflammatory cytokines. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically in sixty-four rats. Neuropathic pain was measured as the withdrawal thresholds of mechanoallodynia and thermal hyperalgesia. On post-CCI days 1, 3, 7 and 14, expression of TNFa and IL-6 was measured bilaterally in the lumbar (L4-L5) and cervical (C7-C8) DRG using immunohistochemistry, western blot analysis, ELISA and in situ hybridization. In addition, the tracers FluoroRuby and FluoroEmerald were injected intrathecally at the level of lumbar or cervical spinal segments. Although mechanoallodynia and thermal hyperalgesia were detected predominantly in the ipsilateral hind paws, the expression of cytokines was enhanced in both the ipsilateral and contralateral cervical and lumbar DRG. The fluorescent tracers diffused from the intrathecal space into the DRG at the injection site as well as remote spinal segments. Our results indicate that cytokines are upregulated bilaterally both in the DRG of the spinal segments that are associated with the injured nerve and in the DRG of remote segments. Therefore, cerebrospinal fluid in the spinal intrathecal space is a putative pathway for signaling molecules to induce changes in proinflammatory cytokines in remote DRG. In conclusion, the upregulation of TNFa and IL-6 in the DRG non-associated with injured nerve suggests that neuroinflammation per se is not correlated strictly with neuropathic pain and is likely a manifestation of general preconditioning to the nervous tissue injury.

Návaznosti

CZ.1.05/1.1.00/02.0068, interní kód MU

Název: CEITEC - středoevropský technologický institut (Akronym: CEITEC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC - středoevropský technologický institut, 1.1 Evropská centra excelence

Citovat

DUBOVÝ, Petr; Ivana HRADILOVÁ SVÍŽENSKÁ; Ilona KLUSÁKOVÁ; Václav BRÁZDA; Marek JOUKAL a Lucie STREJČKOVÁ. Upregulation of TNFa and IL-6 in both the ipsilateral and contralateral dorsal root ganglia associated and non-associated with unilateral nerve injury: A possible role for intrathecal signaling. In FENS Forum of Neuroscience Barcelona. 2012.

@proceedings{1084909,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Joukal, Marek and Strejčková, Lucie},
   booktitle = {FENS Forum of Neuroscience Barcelona},
   keywords = {TNFa; IL-6; dorsal root ganglia; unilateral nerve injury; signaling pathway of neuroinflammation},
   title = {Upregulation of TNFa and IL-6 in both the ipsilateral and contralateral dorsal root ganglia associated and non-associated with unilateral nerve injury: A possible role for intrathecal signaling},
   url = {http://fens.ekonnect.co/FENS_331},
   year = {2012}
}

TY  - CONF
ID  - 1084909
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Joukal, Marek - Strejčková, Lucie
PY  - 2012
TI  - Upregulation of TNFa and IL-6 in both the ipsilateral and contralateral dorsal root ganglia associated and non-associated with unilateral nerve injury: A possible role for intrathecal signaling
KW  - TNFa
KW  - IL-6
KW  - dorsal root ganglia
KW  - unilateral nerve injury
KW  - signaling pathway of neuroinflammation
UR  - http://fens.ekonnect.co/FENS_331
N2  - Following mechanical injury to a peripheral nerve, Wallerian degeneration is an essential stimulus for inducing the cellular and molecular changes that underlie neuropathic pain. Compelling data suggest that hyperalgesia, allodynia and ongoing pain due to peripheral nerve injury are associated with neuroinflammation in the dorsal root ganglia (DRG), which is characterized by an upregulation of pro- and anti-inflammatory cytokines. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically in sixty-four rats. Neuropathic pain was measured as the withdrawal thresholds of mechanoallodynia and thermal hyperalgesia. On post-CCI days 1, 3, 7 and 14, expression of TNFa and IL-6 was measured bilaterally in the lumbar (L4-L5) and cervical (C7-C8) DRG using immunohistochemistry, western blot analysis, ELISA and in situ hybridization. In addition, the tracers FluoroRuby and FluoroEmerald were injected intrathecally at the level of lumbar or cervical spinal segments. Although mechanoallodynia and thermal hyperalgesia were detected predominantly in the ipsilateral hind paws, the expression of cytokines was enhanced in both the ipsilateral and contralateral cervical and lumbar DRG. The fluorescent tracers diffused from the intrathecal space into the DRG at the injection site as well as remote spinal segments. Our results indicate that cytokines are upregulated bilaterally both in the DRG of the spinal segments that are associated with the injured nerve and in the DRG of remote segments. Therefore, cerebrospinal fluid in the spinal intrathecal space is a putative pathway for signaling molecules to induce changes in proinflammatory cytokines in remote DRG. In conclusion, the upregulation of TNFa and IL-6 in the DRG non-associated with injured nerve suggests that neuroinflammation per se is not correlated strictly with neuropathic pain and is likely a manifestation of general preconditioning to the nervous tissue injury.
ER  -

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