Bilateral propagation of neuroinflammatory reaction in the
dorsal root ganglia alongside neuroaxis after unilateral nerve
injury and possible intrathecal signaling

a 2012

Bilateral propagation of neuroinflammatory reaction in the dorsal root ganglia alongside neuroaxis after unilateral nerve injury and possible intrathecal signaling

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL et. al.

Základní údaje

Originální název

Bilateral propagation of neuroinflammatory reaction in the dorsal root ganglia alongside neuroaxis after unilateral nerve injury and possible intrathecal signaling

Název česky

Bilaterální šíření neurozánětlivé reakce ve spinálních gangliích potkana podél neuroaxis po jednostranném poškození periferního nervu a případné intratekální signalizaci

Autoři

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL a Lucie STREJČKOVÁ

Vydání

14th International Neuroscience Winter Conference Solden, Austria, 2012

Další údaje

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

jednostranné poškození periferního nervu; TNFa; IL-6; spinální ganglia; signalizace v nervové soustavě

Klíčová slova anglicky

unilateral chronic constriction injury of the peripheral nerve; TNFa; IL-6; dorsal root ganglia; intrathecal signalization

Příznaky

Mezinárodní význam

Změněno: 23. 12. 2013 15:19, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

Wallerian degeneration following mechanical injury of the peripheral nerve is essential stimulus for cellular and molecular changes inducing neuropathic pain. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically in sixty fore rats. Neuropathic pain induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of TNFa and IL-6 protein as well as mRNA was investigated bilaterally by immunohistochemistry, Western blot, ELISA and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG following CCI for 1, 3, 7 and 14 days. In addition, FluoroRuby (dextran-TRITC) was injected intrathecally in the level of L4-L5 or C7-C8 spinal segments. Although mechanoallodynia and thermal hyperalgesia were detected predominantly in the ipsilateral hind paws from 1 to 14 days, levels of cytokine proteins and mRNA were enhanced bilaterally in both cervical and lumbar DRG. FluoroRuby penetrated from intrathecal space not only into DRG at the level of application but diffused also into DRG of remote spinal segments. Our results indicate that cytokine proteins and their synthesis in DRG may spread bilaterally from the spinal segments associated with injured nerve and per se are not completely involved in neuropathic pain induction and maintenance. A possible pathway for diffusion of signal molecules inducing changes in the remote DRG is cerebrospinal fluid of spinal intrathecal space.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL a Lucie STREJČKOVÁ. Bilateral propagation of neuroinflammatory reaction in the dorsal root ganglia alongside neuroaxis after unilateral nerve injury and possible intrathecal signaling. In 14th International Neuroscience Winter Conference Solden, Austria. 2012.

@proceedings{1085468,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Joukal, Marek and Strejčková, Lucie},
   booktitle = {14th International Neuroscience Winter Conference Solden, Austria},
   keywords = {unilateral chronic constriction injury of the peripheral nerve; TNFa; IL-6; dorsal root ganglia; intrathecal signalization},
   title = {Bilateral propagation of neuroinflammatory reaction in the dorsal root ganglia alongside neuroaxis after unilateral nerve injury and possible intrathecal signaling},
   year = {2012}
}

TY  - CONF
ID  - 1085468
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Joukal, Marek - Strejčková, Lucie
PY  - 2012
TI  - Bilateral propagation of neuroinflammatory reaction in the dorsal root ganglia alongside neuroaxis after unilateral nerve injury and possible intrathecal signaling
KW  - unilateral chronic constriction injury of the peripheral nerve
KW  - TNFa
KW  - IL-6
KW  - dorsal root ganglia
KW  - intrathecal signalization
N2  - Wallerian degeneration following mechanical injury of the peripheral nerve is essential stimulus for cellular and molecular changes inducing neuropathic pain. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically in sixty fore rats. Neuropathic pain induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Expression of TNFa and IL-6 protein as well as mRNA was investigated bilaterally by immunohistochemistry, Western blot, ELISA and in situ hybridization in both lumbar (L4-5) and cervical (C7-8) DRG following CCI for 1, 3, 7 and 14 days. In addition, FluoroRuby (dextran-TRITC) was injected intrathecally in the level of L4-L5 or C7-C8 spinal segments. Although mechanoallodynia and thermal hyperalgesia were detected predominantly in the ipsilateral hind paws from 1 to 14 days, levels of cytokine proteins and mRNA were enhanced bilaterally in both cervical and lumbar DRG. FluoroRuby penetrated from intrathecal space not only into DRG at the level of application but diffused also into DRG of remote spinal segments. Our results indicate that cytokine proteins and their synthesis in DRG may spread bilaterally from the spinal segments associated with injured nerve and per se are not completely involved in neuropathic pain induction and maintenance. A possible pathway for diffusion of signal molecules inducing changes in the remote DRG is cerebrospinal fluid of spinal intrathecal space.
ER  -

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL a Lucie STREJČKOVÁ. Bilateral propagation of neuroinflammatory reaction in the dorsal root ganglia alongside neuroaxis after unilateral nerve injury and possible intrathecal signaling. In \textit{14th International Neuroscience Winter Conference Solden, Austria}. 2012.

Podrobný výpis o publikaci