HUSKA, Dalibor, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Tomáš ECKSCHLAGER, Marie STIBOROVÁ, Ivo PROVAZNIK a René KIZEK. Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction. Chimica Oggi/Chemistry today. 2010, roč. 28, č. 5, s. 18-20. ISSN 1973-8250. |
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@article{1085566, author = {Huska, Dalibor and Adam, Vojtech and Hubálek, Jaromír and Trnková, Libuše and Eckschlager, Tomáš and Stiborová, Marie and Provaznik, Ivo and Kizek, René}, article_number = {5}, keywords = {ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation}, language = {eng}, issn = {1973-8250}, journal = {Chimica Oggi/Chemistry today}, title = {Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction}, volume = {28}, year = {2010} }
TY - JOUR ID - 1085566 AU - Huska, Dalibor - Adam, Vojtech - Hubálek, Jaromír - Trnková, Libuše - Eckschlager, Tomáš - Stiborová, Marie - Provaznik, Ivo - Kizek, René PY - 2010 TI - Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction JF - Chimica Oggi/Chemistry today VL - 28 IS - 5 SP - 18-20 EP - 18-20 SN - 19738250 KW - ellipticine KW - DNA KW - square wave voltammetry KW - adsorptive transfer technique KW - automation N2 - Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations. ER -
HUSKA, Dalibor, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ, Tomáš ECKSCHLAGER, Marie STIBOROVÁ, Ivo PROVAZNIK a René KIZEK. Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction. \textit{Chimica Oggi/Chemistry today}. 2010, roč.~28, č.~5, s.~18-20. ISSN~1973-8250.
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