Off-line coupling of automated pipetting system with square
wave voltammetry as a tool for study of drug-DNA interaction

J 2010

Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction

HUSKA, Dalibor; Vojtech ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Tomáš ECKSCHLAGER et. al.

Základní údaje

Originální název

Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction

Autoři

HUSKA, Dalibor (203 Česká republika); Vojtech ADAM (203 Česká republika); Jaromír HUBÁLEK (203 Česká republika); Libuše TRNKOVÁ (203 Česká republika, domácí); Tomáš ECKSCHLAGER (203 Česká republika); Marie STIBOROVÁ (203 Česká republika); Ivo PROVAZNIK (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

Chimica Oggi/Chemistry today, 2010, 1973-8250

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/10:00063547

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000283582200005

Klíčová slova anglicky

ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation

Štítky

AKR, rivok

Změněno: 6. 2. 2013 10:11, Mgr. Sylvie Dohnalíková

Anotace

V originále

Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations.

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

HUSKA, Dalibor; Vojtech ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Tomáš ECKSCHLAGER; Marie STIBOROVÁ; Ivo PROVAZNIK a René KIZEK. Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction. Chimica Oggi/Chemistry today. 2010, roč. 28, č. 5, s. 18-20. ISSN 1973-8250.

@article{1085566,
   author = {Huska, Dalibor and Adam, Vojtech and Hubálek, Jaromír and Trnková, Libuše and Eckschlager, Tomáš and Stiborová, Marie and Provaznik, Ivo and Kizek, René},
   article_number = {5},
   keywords = {ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation},
   language = {eng},
   issn = {1973-8250},
   journal = {Chimica Oggi/Chemistry today},
   title = {Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction},
   volume = {28},
   year = {2010}
}

TY  - JOUR
ID  - 1085566
AU  - Huska, Dalibor - Adam, Vojtech - Hubálek, Jaromír - Trnková, Libuše - Eckschlager, Tomáš - Stiborová, Marie - Provaznik, Ivo - Kizek, René
PY  - 2010
TI  - Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction
JF  - Chimica Oggi/Chemistry today
VL  - 28
IS  - 5
SP  - 18-20
EP  - 18-20
SN  - 19738250
KW  - ellipticine
KW  - DNA
KW  - square wave voltammetry
KW  - adsorptive transfer technique
KW  - automation
N2  - Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations.
ER  -

HUSKA, Dalibor; Vojtech ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Tomáš ECKSCHLAGER; Marie STIBOROVÁ; Ivo PROVAZNIK a René KIZEK. Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction. \textit{Chimica Oggi/Chemistry today}. 2010, roč.~28, č.~5, s.~18-20. ISSN~1973-8250.

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