Biocompatibility of a genetically encoded calcium indicator in
a transgenic mouse model

J 2012

Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model

DIRENBERGER, Tephan, Marsilius MUES, Vincenzo MICALE, Carsten T WOTJAK, Steffen DIETZEL et. al.

Základní údaje

Originální název

Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model

Autoři

Vydání

NATURE COMMUNICATIONS, LONDON, NATURE PUBLISHING GROUP, 2012, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.015

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/ncomms2035

UT WoS

000308801100046

Klíčová slova anglicky

GREEN FLUORESCENT PROTEIN; TROPONIN-C; IN-VIVO; SINOATRIAL NODE; RESISTANCE ARTERIES; CA2+ INDICATORS; RHOA/RHO-KINASE; MICE; EXPRESSION; ACTIVATION

Štítky

ne MU

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 3. 2015 09:23, Olga Křížová

Anotace

V originále

Engineering efforts of genetically encoded calcium indicators predominantly focused on enhancing fluorescence changes, but how indicator expression affects the physiology of host organisms is often overlooked. Here, we demonstrate biocompatibility and widespread functional expression of the genetically encoded calcium indicator TN-XXL in a transgenic mouse model. To validate the model and characterize potential effects of indicator expression we assessed both indicator function and a variety of host parameters, such as anatomy, physiology, behaviour and gene expression profiles in these mice. We also demonstrate the usefulness of primary cells and organ explants prepared from these mice for imaging applications. Although we find mild signatures of indicator expression that may be further reduced in future sensor generations, the 'green' indicator mice generated provide a well-characterized resource of primary cells and tissues for in vitro and in vivo calcium imaging applications.

Citovat

DIRENBERGER, Tephan, Marsilius MUES, Vincenzo MICALE, Carsten T WOTJAK, Steffen DIETZEL, Michael SCHUBERT, Andreas SCHARR, Sami HASSAN, Christian WAHL-SCHOTT, Martin BIEL, Gurumoorthy KRISHNAMOORTHY a Oliver GRIESBECK. Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model. NATURE COMMUNICATIONS. LONDON: NATURE PUBLISHING GROUP, 2012, roč. 3, č. 1031, s. "nestránkováno", 10 s. ISSN 2041-1723. Dostupné z: https://dx.doi.org/10.1038/ncomms2035.

@article{1086857,
   author = {Direnberger, Tephan and Mues, Marsilius and Micale, Vincenzo and Wotjak, Carsten T and Dietzel, Steffen and Schubert, Michael and Scharr, Andreas and Hassan, Sami and WahlandSchott, Christian and Biel, Martin and Krishnamoorthy, Gurumoorthy and Griesbeck, Oliver},
   article_location = {LONDON},
   article_number = {1031},
   doi = {http://dx.doi.org/10.1038/ncomms2035},
   keywords = {GREEN FLUORESCENT PROTEIN; TROPONIN-C; IN-VIVO; SINOATRIAL NODE; RESISTANCE ARTERIES; CA2+ INDICATORS; RHOA/RHO-KINASE; MICE; EXPRESSION; ACTIVATION},
   language = {eng},
   issn = {2041-1723},
   journal = {NATURE COMMUNICATIONS},
   title = {Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model},
   url = {http://www.nature.com/ncomms/journal/v3/n8/pdf/ncomms2035.pdf?WT.ec_id=NCOMMS-20120828},
   volume = {3},
   year = {2012}
}

TY  - JOUR
ID  - 1086857
AU  - Direnberger, Tephan - Mues, Marsilius - Micale, Vincenzo - Wotjak, Carsten T - Dietzel, Steffen - Schubert, Michael - Scharr, Andreas - Hassan, Sami - Wahl-Schott, Christian - Biel, Martin - Krishnamoorthy, Gurumoorthy - Griesbeck, Oliver
PY  - 2012
TI  - Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model
JF  - NATURE COMMUNICATIONS
VL  - 3
IS  - 1031
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - NATURE PUBLISHING GROUP
SN  - 20411723
KW  - GREEN FLUORESCENT PROTEIN
KW  - TROPONIN-C
KW  - IN-VIVO
KW  - SINOATRIAL NODE
KW  - RESISTANCE ARTERIES
KW  - CA2+ INDICATORS
KW  - RHOA/RHO-KINASE
KW  - MICE
KW  - EXPRESSION
KW  - ACTIVATION
UR  - http://www.nature.com/ncomms/journal/v3/n8/pdf/ncomms2035.pdf?WT.ec_id=NCOMMS-20120828
N2  - Engineering efforts of genetically encoded calcium indicators predominantly focused on enhancing fluorescence changes, but how indicator expression affects the physiology of host organisms is often overlooked. Here, we demonstrate biocompatibility and widespread functional expression of the genetically encoded calcium indicator TN-XXL in a transgenic mouse model. To validate the model and characterize potential effects of indicator expression we assessed both indicator function and a variety of host parameters, such as anatomy, physiology, behaviour and gene expression profiles in these mice. We also demonstrate the usefulness of primary cells and organ explants prepared from these mice for imaging applications. Although we find mild signatures of indicator expression that may be further reduced in future sensor generations, the 'green' indicator mice generated provide a well-characterized resource of primary cells and tissues for in vitro and in vivo calcium imaging applications.
ER  -

DIRENBERGER, Tephan, Marsilius MUES, Vincenzo MICALE, Carsten T WOTJAK, Steffen DIETZEL, Michael SCHUBERT, Andreas SCHARR, Sami HASSAN, Christian WAHL-SCHOTT, Martin BIEL, Gurumoorthy KRISHNAMOORTHY a Oliver GRIESBECK. Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model. \textit{NATURE COMMUNICATIONS}. LONDON: NATURE PUBLISHING GROUP, 2012, roč.~3, č.~1031, s.~''nestránkováno'', 10 s. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/ncomms2035.

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