Variability, structure and selection of MHCIIB genes in
Chondrostoma toxostoma and Chondrostoma nasus in hybrid zones

a 2012

Variability, structure and selection of MHCIIB genes in Chondrostoma toxostoma and Chondrostoma nasus in hybrid zones

CIVÁŇOVÁ, Kristína, Andrea VETEŠNÍKOVÁ ŠIMKOVÁ, Caroline COSTEDOAT, André GILLES, Jean-Francois MARTIN et. al.

Základní údaje

Originální název

Variability, structure and selection of MHCIIB genes in Chondrostoma toxostoma and Chondrostoma nasus in hybrid zones

Autoři

CIVÁŇOVÁ, Kristína (703 Slovensko, garant, domácí), Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Česká republika, domácí), Caroline COSTEDOAT (250 Francie), André GILLES (250 Francie) a Jean-Francois MARTIN (250 Francie)

Vydání

XIV. European Congres of Ichthyology, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Belgie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/12:00058586

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MHC IIB genes; Chondrostoma; endemic and invasive species; hybridization; genetic diversity; population structure; selection

Změněno: 27. 2. 2013 22:00, RNDr. Kristína Křížová, Ph.D.

Anotace

V originále

To characterise the overall genetic diversity, population structure and selection processes in cyprinid fish, MHC genes of IIB class have been analyzed. As the model organism in this study, two congeneric cyprinids, the introduced Chondrostoma nasus and native, protected and endemic C. toxostoma that live in sympatry and form a hybrid zone in the Durance River (South France, Mediterranean Sea drainage) were studied. For the individual determination (pure species versus hybrid) all fish were genotyped for mtDNA (cyt b) and 41 microsatellite loci. The allelic variability in exon 2 of two MHCIIB genes (DAB1-like and DAB3-like) representing the most variable region with the nonsynonymous sites specifying the peptide binding region was analysed. The results reveal the non uniform allele expression within individuals, duplication of both studied loci (previously showed in several cyprinid species) and that the exon 2 of DAB genes is subjected to positive selection. The difference in the MHCIIB profiles was found between allopatric and sympatric populations. Based on the hypothesis of parasite-mediated selection maintaining MHC polymorphism it could suggest the difference in parasite load between chondrostomes living in allopatric area and those living in hybrid zone.

Návaznosti

GAP505/10/1138, projekt VaV

Název: Hostitelsko-parazitické interakce v hybridních systémech kaprovitých ryb

Investor: Grantová agentura ČR, Hostitelsko-parazitické interakce v hybridních systémech kaprovitých ryb

Citovat

CIVÁŇOVÁ, Kristína, Andrea VETEŠNÍKOVÁ ŠIMKOVÁ, Caroline COSTEDOAT, André GILLES a Jean-Francois MARTIN. Variability, structure and selection of MHCIIB genes in Chondrostoma toxostoma and Chondrostoma nasus in hybrid zones. In XIV. European Congres of Ichthyology. 2012.

@proceedings{1089493,
   author = {Civáňová, Kristína and Vetešníková Šimková, Andrea and Costedoat, Caroline and Gilles, André and Martin, JeanandFrancois},
   booktitle = {XIV. European Congres of Ichthyology},
   keywords = {MHC IIB genes; Chondrostoma; endemic and invasive species; hybridization; genetic diversity; population structure; selection},
   language = {eng},
   title = {Variability, structure and selection of MHCIIB genes in Chondrostoma toxostoma and Chondrostoma nasus in hybrid zones},
   year = {2012}
}

TY  - CONF
ID  - 1089493
AU  - Civáňová, Kristína - Vetešníková Šimková, Andrea - Costedoat, Caroline - Gilles, André - Martin, Jean-Francois
PY  - 2012
TI  - Variability, structure and selection of MHCIIB genes in Chondrostoma toxostoma and Chondrostoma nasus in hybrid zones
KW  - MHC IIB genes
KW  - Chondrostoma
KW  - endemic and invasive species
KW  - hybridization
KW  - genetic diversity
KW  - population structure
KW  - selection
N2  - To characterise the overall genetic diversity, population structure and selection processes in cyprinid fish, MHC genes of IIB class have been analyzed. As the model organism in this study, two congeneric cyprinids, the introduced Chondrostoma nasus and native, protected and endemic C. toxostoma that live in sympatry and form a hybrid zone in the Durance River (South France, Mediterranean Sea drainage) were studied. For the individual determination (pure species versus hybrid) all fish were genotyped for mtDNA (cyt b) and 41 microsatellite loci. The allelic variability in exon 2 of two MHCIIB genes (DAB1-like and DAB3-like) representing the most variable region with the nonsynonymous sites specifying the peptide binding region was analysed. The results reveal the non uniform allele expression within individuals, duplication of both studied loci (previously showed in several cyprinid species) and that the exon 2 of DAB genes is subjected to positive selection. The difference in the MHCIIB profiles was found between allopatric and sympatric populations. Based on the hypothesis of parasite-mediated selection maintaining MHC polymorphism it could suggest the difference in parasite load between chondrostomes living in allopatric area and those living in hybrid zone.
ER  -

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