The variability and expression of MHC genes and their rolein host-parasite interactions

CIVÁŇOVÁ, Kristína. The variability and expression of MHC genes and their rolein host-parasite interactions. In Workshop ECIP 2012, Šatov. 2012.

Základní údaje

Originální název

The variability and expression of MHC genes and their rolein host-parasite interactions

Autoři

CIVÁŇOVÁ, Kristína ORCID

Vydání

Workshop ECIP 2012, Šatov, 2012

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Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/12:00058592

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MHC IIB genes; 454 pyrosequencing; qPCR; HRM

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Anotace

V originále

The contribution of immunoecological studies has gained a place of central importance in recent evolutionary biology and ecology. The main aim of the work is to analyze the associations between multiple parasites and host immunocompetence and to investigate the structure and evolutionary mechanism of selection in MHC genes of cyprinid fish species. The extremely high level of allelic diversity in the genes of major histocompatibility complex results of many mechanisms and is determined with the intent to obtain genetic data for evolutionary and population studies. Nowadays, for MHC genotyping we use two basic approaches: 454 clonal pyrosequencing of amplicons and CE-SSCP of amplicon clones combined with automatic sequencing based on Sanger technology. In this contribution, we present primary results of a new approach for MHC genes variability tests – high resolution melting analysis (HRM) and expression studies (using qPCR), that enable us to extend the studies on MHC genes up to functional level.

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Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie