a 2012

The variability and expression of MHC genes and their rolein host-parasite interactions

CIVÁŇOVÁ, Kristína

Basic information

Original name

The variability and expression of MHC genes and their rolein host-parasite interactions

Authors

CIVÁŇOVÁ, Kristína (703 Slovakia, guarantor, belonging to the institution)

Edition

Workshop ECIP 2012, Šatov, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/12:00058592

Organization unit

Faculty of Science

Keywords in English

MHC IIB genes; 454 pyrosequencing; qPCR; HRM
Změněno: 28/2/2013 14:03, RNDr. Kristína Křížová, Ph.D.

Abstract

V originále

The contribution of immunoecological studies has gained a place of central importance in recent evolutionary biology and ecology. The main aim of the work is to analyze the associations between multiple parasites and host immunocompetence and to investigate the structure and evolutionary mechanism of selection in MHC genes of cyprinid fish species. The extremely high level of allelic diversity in the genes of major histocompatibility complex results of many mechanisms and is determined with the intent to obtain genetic data for evolutionary and population studies. Nowadays, for MHC genotyping we use two basic approaches: 454 clonal pyrosequencing of amplicons and CE-SSCP of amplicon clones combined with automatic sequencing based on Sanger technology. In this contribution, we present primary results of a new approach for MHC genes variability tests – high resolution melting analysis (HRM) and expression studies (using qPCR), that enable us to extend the studies on MHC genes up to functional level.

Links

GBP505/12/G112, research and development project
Name: ECIP - Evropské centrum ichtyoparazitologie
Investor: Czech Science Foundation