Proteome regulation in Arabidopsis reveals shoot- and
root-specific effects of cytokinins on hormonal homeostasis and
cytokinin-ethylene signaling crosstalk

a 2012

Proteome regulation in Arabidopsis reveals shoot- and root-specific effects of cytokinins on hormonal homeostasis and cytokinin-ethylene signaling crosstalk

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, Mariana BENITEZ KEINRAD, Ondrej ŠEDO, Tereza DOBISOVÁ et. al.

Základní údaje

Originální název

Proteome regulation in Arabidopsis reveals shoot- and root-specific effects of cytokinins on hormonal homeostasis and cytokinin-ethylene signaling crosstalk

Autoři

Vydání

XXIII. Biochemický sjezd, 2012

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

Klíčová slova česky

Arabidopsis, proteom, cytokinin, ethylén, interakce

Klíčová slova anglicky

Arabidopsis, proteome, cytokinin, ethylene, crosstalk

Změněno: 3. 4. 2013 10:13, Mgr. Markéta Žďárská, Ph.D.

Anotace

V originále

Cytokinins (CK) mediate a large spectrum of developmental and physiological regulations in plants. Shoot and root reveal specific and sometimes even contrary responses to CK. However, the molecular mechanisms underlying the shoot and root specificity of CK-mediated regulations remain mostly unclear. We show that differential CK-mediated proteome regulation underlies the shoot and root specificity of CK response at the hormonal metabolism and signaling levels. Overall, we identified 43/18 differentially regulated proteins in the shoot and 31/21 in the root in the early/delayed response, respectively. We demonstrate that CK predominantly regulate proteins involved in carbohydrate and energy metabolism in the shoot and in protein synthesis and destination in the root. In the root we identified 3 out of 4 enzymes of the ethylene biosynthetic pathway to be upregulated in an early CK response, suggesting that majority of the ethylene biosynthetic pathway is under strong control of CK in the root. On the other hand, in the shoot we identified several abscisic acid (ABA)-related proteins to be upregulated by CK.To substantiate potential crosstalk of BAP with metabolism of other hormones, we analyzed changes of the endogenous hormone levels after CK application. In a good accordance with our proteomic data, we found prompt upregulation of 1-aminocyclopropane-1-carboxylic acid, the rate-limiting precursor of ethylene biosynthesis in the root, while ABA was upregulated in the early response of the shoot. Furthermore, we show that CK-induced ethylene production contributes to CK response in the root and modulates multistep phosphorelay (MSP) output through the direct interaction of ethylene receptor ETR1 with AHP proteins, the members of the MSP signaling cascade. Finally, we demonstrate that MSP mediates ethylene response, thus integrating both CK and ethylene signaling.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, Mariana BENITEZ KEINRAD, Ondrej ŠEDO, Tereza DOBISOVÁ, Vendula HRDINOVÁ, David POTĚŠIL, Ondřej NOVÁK, Jana SVAČINOVÁ, Bedřich PEŠEK, Jiří MALBECK, Jana VAŠÍČKOVÁ, Eva MIKLÁNKOVÁ, Zbyněk ZDRÁHAL a Jan HEJÁTKO. Proteome regulation in Arabidopsis reveals shoot- and root-specific effects of cytokinins on hormonal homeostasis and cytokinin-ethylene signaling crosstalk. In XXIII. Biochemický sjezd. 2012.

@proceedings{1092577,
   author = {Žďárská, Markéta and Zatloukalová, Pavlína and Benitez Keinrad, Mariana and Šedo, Ondrej and Dobisová, Tereza and Hrdinová, Vendula and Potěšil, David and Novák, Ondřej and Svačinová, Jana and Pešek, Bedřich and Malbeck, Jiří and Vašíčková, Jana and Miklánková, Eva and Zdráhal, Zbyněk and Hejátko, Jan},
   booktitle = {XXIII. Biochemický sjezd},
   keywords = {Arabidopsis, proteome, cytokinin, ethylene, crosstalk},
   language = {eng},
   title = {Proteome regulation in Arabidopsis reveals shoot- and root-specific effects of cytokinins on hormonal homeostasis and cytokinin-ethylene signaling crosstalk},
   year = {2012}
}

TY  - CONF
ID  - 1092577
AU  - Žďárská, Markéta - Zatloukalová, Pavlína - Benitez Keinrad, Mariana - Šedo, Ondrej - Dobisová, Tereza - Hrdinová, Vendula - Potěšil, David - Novák, Ondřej - Svačinová, Jana - Pešek, Bedřich - Malbeck, Jiří - Vašíčková, Jana - Miklánková, Eva - Zdráhal, Zbyněk - Hejátko, Jan
PY  - 2012
TI  - Proteome regulation in Arabidopsis reveals shoot- and root-specific effects of cytokinins on hormonal homeostasis and cytokinin-ethylene signaling crosstalk
KW  - Arabidopsis, proteome, cytokinin, ethylene, crosstalk
N2  - Cytokinins (CK) mediate a large spectrum of developmental and physiological regulations in plants. Shoot and root reveal specific and sometimes even contrary responses to CK. However, the molecular mechanisms underlying the shoot and root specificity of CK-mediated regulations remain mostly unclear. We show that differential CK-mediated proteome regulation underlies the shoot and root specificity of CK response at the hormonal metabolism and signaling levels. Overall, we identified 43/18 differentially regulated proteins in the shoot and 31/21 in the root in the early/delayed response, respectively. We demonstrate that CK predominantly regulate proteins involved in carbohydrate and energy metabolism in the shoot and in protein synthesis and destination in the root. In the root we identified 3 out of 4 enzymes of the ethylene biosynthetic pathway to be upregulated in an early CK response, suggesting that majority of the ethylene biosynthetic pathway is under strong control of CK in the root. On the other hand, in the shoot we identified several abscisic acid (ABA)-related proteins to be upregulated by CK.To substantiate potential crosstalk of BAP with metabolism of other hormones, we analyzed changes of the endogenous hormone levels after CK application. In a good accordance with our proteomic data, we found prompt upregulation of 1-aminocyclopropane-1-carboxylic acid, the rate-limiting precursor of ethylene biosynthesis in the root, while ABA was upregulated in the early response of the shoot. Furthermore, we show that CK-induced ethylene production contributes to CK response in the root and modulates multistep phosphorelay (MSP) output through the direct interaction of ethylene receptor ETR1 with AHP proteins, the members of the MSP signaling cascade. Finally, we demonstrate that MSP mediates ethylene response, thus integrating both CK and ethylene signaling.
ER  -

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