Histone H2A.Z inheritance during the cell cycle and its impact
on promoter organization and dynamics

J 2012

Histone H2A.Z inheritance during the cell cycle and its impact on promoter organization and dynamics

NEKRASOV, M; Jana AMRICHOVÁ; BJ PARKER; TA SOBOLEVA; C JACK et. al.

Základní údaje

Originální název

Histone H2A.Z inheritance during the cell cycle and its impact on promoter organization and dynamics

Název česky

Výskyt histonu H2A.Z během buněčného cyklu a jeho vliv na strukturu a dynamiku promotoru

Autoři

NEKRASOV, M; Jana AMRICHOVÁ; BJ PARKER; TA SOBOLEVA; C JACK; R WILLIAMS; GA HUTTLEY a DJ TREMETHICK

Vydání

NATURE STRUCTURAL & MOLECULAR BIOLOGY, United States, Nature Pub. Group, 2012, 1545-9985

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

DOI

https://doi.org/10.1038/nsmb.2424

UT WoS

000310788800005

Klíčová slova česky

histony, H2A.Z, buněčný cyklus, struktura promotoru, nukleosomy

Klíčová slova anglicky

histone, H2A.Z, cell cycle, promoter organisation, nucleosomes

Štítky

cell cycle, H2A Z, histone, nucleosomes, promoter organisation

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 3. 2013 19:45, Mgr. Ing. Jana Amrichová, Ph.D.

Anotace

V originále

Although it has been clearly established that well-positioned histone H2A.Z-containing nucleosomes flank the nucleosome-depleted region (NDR) at the transcriptional start site (TSS) of active mammalian genes, how this chromatin-based information is transmitted through the cell cycle is unknown. We show here that in mouse trophoblast stem cells, the amount of histone H2A.Z at promoters decreased during S phase, coinciding with homotypic (H2A.Z-H2A.Z) nucleosomes flanking the TSS becoming heterotypic (H2A.Z-H2A). To our surprise these nucleosomes remained heterotypic at M phase. At the TSS, we identified an unstable heterotypic histone H2A.Z-containing nucleosome in G1 phase that was lost after DNA replication. These dynamic changes at the TSS mirror a global expansion of the NDR at S and M phases, which, unexpectedly, is unrelated to transcriptional activity. Coincident with the loss of histone H2A.Z at promoters, histone H2A.Z is targeted to the centromere when mitosis begins.

Citovat

NEKRASOV, M; Jana AMRICHOVÁ; BJ PARKER; TA SOBOLEVA; C JACK; R WILLIAMS; GA HUTTLEY a DJ TREMETHICK. Histone H2A.Z inheritance during the cell cycle and its impact on promoter organization and dynamics. NATURE STRUCTURAL & MOLECULAR BIOLOGY. United States: Nature Pub. Group,, 2012, s. 1076-1083. ISSN 1545-9985. Dostupné z: https://doi.org/10.1038/nsmb.2424.

@article{1093472,
   author = {Nekrasov, M and Amrichová, Jana and Parker, BJ and Soboleva, TA and Jack, C and Williams, R and Huttley, GA and Tremethick, DJ},
   article_location = {United States},
   doi = {https://doi.org/10.1038/nsmb.2424},
   keywords = {histone, H2A.Z, cell cycle, promoter organisation, nucleosomes},
   issn = {1545-9985},
   journal = {NATURE STRUCTURAL & MOLECULAR BIOLOGY},
   title = {Histone H2A.Z inheritance during the cell cycle and its impact on promoter organization and dynamics},
   year = {2012}
}

TY  - JOUR
ID  - 1093472
AU  - Nekrasov, M - Amrichová, Jana - Parker, BJ - Soboleva, TA - Jack, C - Williams, R - Huttley, GA - Tremethick, DJ
PY  - 2012
TI  - Histone H2A.Z inheritance during the cell cycle and its impact on promoter organization and dynamics
JF  - NATURE STRUCTURAL & MOLECULAR BIOLOGY
SP  - 1076-1083
EP  - 1076-1083
PB  - Nature Pub. Group,
SN  - 15459985
KW  - histone, H2A.Z, cell cycle, promoter organisation, nucleosomes
N2  - Although it has been clearly established that well-positioned histone H2A.Z-containing nucleosomes flank the nucleosome-depleted region (NDR) at the transcriptional start site (TSS) of active mammalian genes, how this chromatin-based information is transmitted through the cell cycle is unknown. We show here that in mouse trophoblast stem cells, the amount of histone H2A.Z at promoters decreased during S phase, coinciding with homotypic (H2A.Z-H2A.Z) nucleosomes flanking the TSS becoming heterotypic (H2A.Z-H2A). To our surprise these nucleosomes remained heterotypic at M phase. At the TSS, we identified an unstable heterotypic histone H2A.Z-containing nucleosome in G1 phase that was lost after DNA replication. These dynamic changes at the TSS mirror a global expansion of the NDR at S and M phases, which, unexpectedly, is unrelated to transcriptional activity. Coincident with the loss of histone H2A.Z at promoters, histone H2A.Z is targeted to the centromere when mitosis begins.
ER  -

NEKRASOV, M; Jana AMRICHOVÁ; BJ PARKER; TA SOBOLEVA; C JACK; R WILLIAMS; GA HUTTLEY a DJ TREMETHICK. Histone H2A.Z inheritance during the cell cycle and its impact on promoter organization and dynamics. \textit{NATURE STRUCTURAL \&{} MOLECULAR BIOLOGY}. United States: Nature Pub. Group,, 2012, s.~1076-1083. ISSN~1545-9985. Dostupné z: https://doi.org/10.1038/nsmb.2424.

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