The effects of histamine and 4-methyl-histamine on the
oxidative burst of human leukocytes

a 2012

The effects of histamine and 4-methyl-histamine on the oxidative burst of human leukocytes

VAŠÍČEK, Ondřej; Lucie PRACHAŘOVÁ; Antonín LOJEK a Milan ČÍŽ

Základní údaje

Originální název

The effects of histamine and 4-methyl-histamine on the oxidative burst of human leukocytes

Autoři

VAŠÍČEK, Ondřej; Lucie PRACHAŘOVÁ; Antonín LOJEK a Milan ČÍŽ

Vydání

41st Annual Meeting, May 2–5, 2012 Belfast, Northern Ireland, 2012

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.964

Označené pro přenos do RIV

ISSN

DOI

https://doi.org/10.1007/s00011-012-0531-x

UT WoS

000308118900050

Klíčová slova anglicky

Histamine, 4-Methyl-Histamin, ROS

Štítky

4-Methyl-Histamin, Histamine, reactive oxygen species

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 7. 2013 13:50, Mgr. Ondřej Vašíček, Ph.D.

Anotace

V originále

Histamine plays an important role in immune system disorders and allergies. Since studies about the relation of the newly discovered histamine H4 receptor with functional properties of phagocytes and phagocyte-derived reactive oxygen species generation are very rare, we focused on evaluating histamine effects on functional activity of human leukocytes and on antioxidant properties of histamine. Heparinized blood from healthy human volunteers was obtained by antecubital venipuncture. Total leukocytes including polymorphonuclear leukocytes were isolated and their ability to produce reactive oxygen species after histamine or its H4 receptor agonist 4-methylhistamine treatment was tested by luminol-enhanced chemiluminescence analysis. Antioxidant properties of histamine and 4-methylhistamine were measured by luminol-enhanced chemiluminescence and fluorescence analyses. Histamine and 4-methylhistamine increased the spontaneous oxidative burst in whole blood phagocytes. Similarly, both compounds increased the oxidative burst of whole blood phagocytes activated by opsonized zymosan particles or phorbol myristate acetate. On the other hand, neither of the compounds affected the oxidative burst in activated isolated leukocytes. The spontaneous oxidative burst of isolated leukocytes was even decreased by both compounds. Our results confirmed that neither histamine nor 4-methylhistamine did have any antioxidant potential against reactive oxygen species. It can be concluded that histamine interacted with immune system cells, especially with phagocytes. The increase in local concentrations of histamine could augment phagocyte-derived oxidative stress. H4 receptors could at least partially play a role in this phenomenon.

Citovat

VAŠÍČEK, Ondřej; Lucie PRACHAŘOVÁ; Antonín LOJEK a Milan ČÍŽ. The effects of histamine and 4-methyl-histamine on the oxidative burst of human leukocytes. In 41st Annual Meeting, May 2–5, 2012 Belfast, Northern Ireland. 2012. ISSN 1023-3830. Dostupné z: https://doi.org/10.1007/s00011-012-0531-x.

@proceedings{1094538,
   author = {Vašíček, Ondřej and Prachařová, Lucie and Lojek, Antonín and Číž, Milan},
   booktitle = {41st Annual Meeting, May 2–5, 2012 Belfast, Northern Ireland},
   doi = {https://doi.org/10.1007/s00011-012-0531-x},
   keywords = {Histamine, 4-Methyl-Histamin, ROS},
   title = {The effects of histamine and 4-methyl-histamine on the oxidative burst of human leukocytes},
   year = {2012}
}

TY  - CONF
ID  - 1094538
AU  - Vašíček, Ondřej - Prachařová, Lucie - Lojek, Antonín - Číž, Milan
PY  - 2012
TI  - The effects of histamine and 4-methyl-histamine on the oxidative burst of human leukocytes
KW  - Histamine, 4-Methyl-Histamin, ROS
N2  - Histamine plays an important role in immune system disorders and allergies. Since studies about the relation of the newly discovered histamine H4 receptor with functional properties of phagocytes and phagocyte-derived reactive oxygen species generation are very rare, we focused on evaluating histamine effects on functional activity of human leukocytes and on antioxidant properties of histamine. Heparinized blood from healthy human volunteers was obtained by antecubital venipuncture. Total leukocytes including polymorphonuclear leukocytes were isolated and their ability to produce reactive oxygen species after histamine or its H4 receptor agonist 4-methylhistamine treatment was tested by luminol-enhanced chemiluminescence analysis. Antioxidant properties of histamine and 4-methylhistamine were measured by luminol-enhanced chemiluminescence and fluorescence analyses. Histamine and 4-methylhistamine increased the spontaneous oxidative burst in whole blood phagocytes. Similarly, both compounds increased the oxidative burst of whole blood phagocytes activated by opsonized zymosan particles or phorbol myristate acetate. On the other hand, neither of the compounds affected the oxidative burst in activated isolated leukocytes. The spontaneous oxidative burst of isolated leukocytes was even decreased by both compounds. Our results confirmed that neither histamine nor 4-methylhistamine did have any antioxidant potential against reactive oxygen species. It can be concluded that histamine interacted with immune system cells, especially with phagocytes. The increase in local concentrations of histamine could augment phagocyte-derived oxidative stress. H4 receptors could at least partially play a role in this phenomenon.
ER  -

VAŠÍČEK, Ondřej; Lucie PRACHAŘOVÁ; Antonín LOJEK a Milan ČÍŽ. The effects of histamine and 4-methyl-histamine on the oxidative burst of human leukocytes. In \textit{41st Annual Meeting, May 2–5, 2012 Belfast, Northern Ireland}. 2012. ISSN~1023-3830. Dostupné z: https://doi.org/10.1007/s00011-012-0531-x.

Přehled o publikaci