Boar sperm head membrane damage during cryopreservation
evaluated by electron microscopy

J 2012

Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy

SEDLÁČKOVÁ, Miroslava; P. PŘINOSILOVÁ; V. KOPECKÁ a J. HLAVICOVÁ

Základní údaje

Originální název

Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy

Autoři

SEDLÁČKOVÁ, Miroslava; P. PŘINOSILOVÁ; V. KOPECKÁ a J. HLAVICOVÁ

Vydání

Research in pig breeding, Kostelec nad Orlicí, Výzkumný ústav živočišné výroby, 2012, 1802-7547

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/12:00068283

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Boar; sperm; membrane; TEM; ultrastructure

Změněno: 15. 5. 2013 15:59, Soňa Böhmová

Anotace

V originále

As electron microscopy can reveal much smaller changes in plasma membrane integrity than optical methods, it appears to be a useful tool for evaluating semen quality or the damage inflicted on spermatozoa during preservation. By evaluating membrane integrity of the sperm head in 16 boars by transmission electron microscopy (TEM), it was found that the plasma membrane is very sensitive and tends to break in the acrosomal area but not in the rest of the sperm head. The earliest signs of injury to spermatozoa in the acrosomal part of the sperm head were observed in the plasma membrane that became swollen, broken or lost. The next damaged part of spermatozoa which is less sensitive than the plasma membrane is the acrosome and its external membrane. The freezing process caused a substantial increase in the degree of cell damage manifested as disturbed or missing plasma membrane, acrosomal reaction-like changes and unevenly distributed or lost acrosomal contents. The latter was not found in fresh semen. Using an appropriate buffer concentration for the fixatives applied during preparation of semen samples for TEM appeared as a very important factor due to the impact of their osmolality on plasma membrane integrity of sperm cells.

Citovat

SEDLÁČKOVÁ, Miroslava; P. PŘINOSILOVÁ; V. KOPECKÁ a J. HLAVICOVÁ. Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy. Research in pig breeding. Kostelec nad Orlicí: Výzkumný ústav živočišné výroby, 2012, roč. 6, č. 2, s. 58-61. ISSN 1802-7547.

@article{1106771,
   author = {Sedláčková, Miroslava and Přinosilová, P. and Kopecká, V. and Hlavicová, J.},
   article_location = {Kostelec nad Orlicí},
   article_number = {č. 2},
   keywords = {Boar; sperm; membrane; TEM; ultrastructure},
   language = {eng},
   issn = {1802-7547},
   journal = {Research in pig breeding},
   title = {Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy},
   volume = {roč. 6},
   year = {2012}
}

TY  - JOUR
ID  - 1106771
AU  - Sedláčková, Miroslava - Přinosilová, P. - Kopecká, V. - Hlavicová, J.
PY  - 2012
TI  - Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy
JF  - Research in pig breeding
VL  - roč. 6
IS  - č. 2
SP  - 58-61
EP  - 58-61
PB  - Výzkumný ústav živočišné výroby
SN  - 18027547
KW  - Boar
KW  - sperm
KW  - membrane
KW  - TEM
KW  - ultrastructure
N2  - As electron microscopy can reveal much smaller changes in plasma membrane integrity than optical methods, it appears to be a useful tool for evaluating semen quality or the damage inflicted on spermatozoa during preservation. By evaluating membrane integrity of the sperm head in 16 boars by transmission electron microscopy (TEM), it was found that the plasma membrane is very sensitive and tends to break in the acrosomal area but not in the rest of the sperm head. The earliest signs of injury to spermatozoa in the acrosomal part of the sperm head were observed in the plasma membrane that became swollen, broken or lost. The next damaged part of spermatozoa which is less sensitive than the plasma membrane is the acrosome and its external membrane. The freezing process caused a substantial increase in the degree of cell damage manifested as disturbed or missing plasma membrane, acrosomal reaction-like changes and unevenly distributed or lost acrosomal contents. The latter was not found in fresh semen. Using an appropriate buffer concentration for the fixatives applied during preparation of semen samples for TEM appeared as a very important factor due to the impact of their osmolality on plasma membrane integrity of sperm cells.
ER  -

SEDLÁČKOVÁ, Miroslava; P. PŘINOSILOVÁ; V. KOPECKÁ a J. HLAVICOVÁ. Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy. \textit{Research in pig breeding}. Kostelec nad Orlicí: Výzkumný ústav živočišné výroby, 2012, roč. 6, č. 2, s.~58-61. ISSN~1802-7547.

Přehled o publikaci