Leptospirosis: possibilities of early laboratory and clinical
diagnosis

J 2013

Leptospirosis: possibilities of early laboratory and clinical diagnosis

ČERMÁKOVÁ, Zuzana; Petra KUČEROVÁ; Zbyněk VALENTA; Lenka PLÍŠKOVÁ; Radka BOLEHOVSKÁ et al.

Základní údaje

Originální název

Leptospirosis: possibilities of early laboratory and clinical diagnosis

Autoři

ČERMÁKOVÁ, Zuzana; Petra KUČEROVÁ; Zbyněk VALENTA; Lenka PLÍŠKOVÁ; Radka BOLEHOVSKÁ; Petr PRÁŠIL; Vladimír BUCHTA; Josef SCHARFEN; Pavel POLÁK; Ota PAVLIS a Barbora VOXOVÁ

Vydání

Central European Journal of Medicine, Warsaw, Versita, 2013, 1895-1058

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30300 3.3 Health sciences

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.209

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/13:00068315

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.2478/s11536-012-0110-0

UT WoS

000312317600014

Klíčová slova anglicky

Leptospirosis; Early diagnosis; PCR and MAT; Clinical symptoms

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 10. 2013 16:05, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

This retrospective study aims to identify and describe the problems associated with the laboratory and clinical diagnosis of leptospirosis. A total of 4,813 patients with suspected leptospirosis from an area of the Czech Republic, with a total population of 1.15 million, were examined during the period 2002-2010. Our study included only 855 patients: 545 men (mean age 41.03 +/- 19.24) and 310 women (mean age 41.47 +/- 20.3) who were examined using microscopic agglutination test (MAT) and a polymerase chain reaction (PCR). All patients and their physicians filled in questionnaires, which included anamnestic data, clinical symptoms and the results of laboratory tests. Out of total suspected, 89 patients (1.85%), tested positive for leptospirosis, of which 50 have been examined only serologically by MAT. Of 855 patients in our study undergoing both PCR and MAT tests, 39 have tested positive for leptospirosis. The most frequent symptom in patients with leptospirosis included fever (91.6%) and headache (69.4%). The correct laboratory diagnosis of leptospirosis depends on biological material being tested before the start of antibiotic treatment, since leptospires are extremely sensitive to antibiotics. Consequently, the PCR results alone may produce a false negative result after 24 hours following treatment with antibiotics.

Přiložené soubory

polak.pdf

Požádat o autorskou verzi souboru

Citovat

ČERMÁKOVÁ, Zuzana; Petra KUČEROVÁ; Zbyněk VALENTA; Lenka PLÍŠKOVÁ; Radka BOLEHOVSKÁ; Petr PRÁŠIL; Vladimír BUCHTA; Josef SCHARFEN; Pavel POLÁK; Ota PAVLIS a Barbora VOXOVÁ. Leptospirosis: possibilities of early laboratory and clinical diagnosis. Central European Journal of Medicine. Warsaw: Versita, 2013, roč. 8, č. 1, s. 84-89. ISSN 1895-1058. Dostupné z: https://doi.org/10.2478/s11536-012-0110-0.

@article{1107009,
   author = {Čermáková, Zuzana and Kučerová, Petra and Valenta, Zbyněk and Plíšková, Lenka and Bolehovská, Radka and Prášil, Petr and Buchta, Vladimír and Scharfen, Josef and Polák, Pavel and Pavlis, Ota and Voxová, Barbora},
   article_location = {Warsaw},
   article_number = {1},
   doi = {https://doi.org/10.2478/s11536-012-0110-0},
   keywords = {Leptospirosis; Early diagnosis; PCR and MAT; Clinical symptoms},
   language = {eng},
   issn = {1895-1058},
   journal = {Central European Journal of Medicine},
   title = {Leptospirosis: possibilities of early laboratory and clinical diagnosis},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1107009
AU  - Čermáková, Zuzana - Kučerová, Petra - Valenta, Zbyněk - Plíšková, Lenka - Bolehovská, Radka - Prášil, Petr - Buchta, Vladimír - Scharfen, Josef - Polák, Pavel - Pavlis, Ota - Voxová, Barbora
PY  - 2013
TI  - Leptospirosis: possibilities of early laboratory and clinical diagnosis
JF  - Central European Journal of Medicine
VL  - 8
IS  - 1
SP  - 84-89
EP  - 84-89
PB  - Versita
SN  - 18951058
KW  - Leptospirosis
KW  - Early diagnosis
KW  - PCR and MAT
KW  - Clinical symptoms
N2  - This retrospective study aims to identify and describe the problems associated with the laboratory and clinical diagnosis of leptospirosis. A total of 4,813 patients with suspected leptospirosis from an area of the Czech Republic, with a total population of 1.15 million, were examined during the period 2002-2010. Our study included only 855 patients: 545 men (mean age 41.03 +/- 19.24) and 310 women (mean age 41.47 +/- 20.3) who were examined using microscopic agglutination test (MAT) and a polymerase chain reaction (PCR). All patients and their physicians filled in questionnaires, which included anamnestic data, clinical symptoms and the results of laboratory tests. Out of total suspected, 89 patients (1.85%), tested positive for leptospirosis, of which 50 have been examined only serologically by MAT. Of 855 patients in our study undergoing both PCR and MAT tests, 39 have tested positive for leptospirosis. The most frequent symptom in patients with leptospirosis included fever (91.6%) and headache (69.4%). The correct laboratory diagnosis of leptospirosis depends on biological material being tested before the start of antibiotic treatment, since leptospires are extremely sensitive to antibiotics. Consequently, the PCR results alone may produce a false negative result after 24 hours following treatment with antibiotics.
ER  -

ČERMÁKOVÁ, Zuzana; Petra KUČEROVÁ; Zbyněk VALENTA; Lenka PLÍŠKOVÁ; Radka BOLEHOVSKÁ; Petr PRÁŠIL; Vladimír BUCHTA; Josef SCHARFEN; Pavel POLÁK; Ota PAVLIS a Barbora VOXOVÁ. Leptospirosis: possibilities of early laboratory and clinical diagnosis. \textit{Central European Journal of Medicine}. Warsaw: Versita, 2013, roč.~8, č.~1, s.~84-89. ISSN~1895-1058. Dostupné z: https://doi.org/10.2478/s11536-012-0110-0.

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