Effect of DNA Dosage into Gene Expression in Patients with
Multiple Myeloma

a 2013

Effect of DNA Dosage into Gene Expression in Patients with Multiple Myeloma

NĚMEC, Pavel, Fedor KRYUKOV, Elena Vladimirovna DEMENTYEVA, Jan SMETANA, Ivana IHNATOVÁ et. al.

Základní údaje

Originální název

Effect of DNA Dosage into Gene Expression in Patients with Multiple Myeloma

Autoři

NĚMEC, Pavel, Fedor KRYUKOV, Elena Vladimirovna DEMENTYEVA, Jan SMETANA, Ivana IHNATOVÁ, Luděk POUR, Petr KUGLÍK a Roman HÁJEK

Vydání

14th International Myeloma Workshop, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.929

Organizační jednotka

Lékařská fakulta

ISSN

Změněno: 20. 5. 2013 12:53, Mgr. Anna Potáčová, Ph.D.

Anotace

V originále

Whole genomic methods represents effective tool for studying genomic changes in cancer cells. Aim of this study was to find and describe genes whose expression is dependent on the DNA copy number (gene dosage) in patients with multiple myeloma. Total of 57 patients with MM were simultaneously examined by arrayCGH for DNA copy number variations (gain/losses) utilizing Agilent Human Genome CGH Microarray 4x44K Arrays and for gene expression utilizing Affymetrix GeneChip Human Gene 1.0 ST. Gene dosage-dependent genes were defined by Spearman correlation[R>0.5, p(FDR)<0.05]of CNV status and expression level and analysed using DAVID Bioinformatics software. Total of 852 fromall 27391 transcripts were strongly and significantly dependent ongene dosage. Cytogeneticaly, majority (25%) of all 852 genes were located on chromosome 1 (with 19 genes mapped to 1q21 locus). Other involved genes were mostly located on chromosomes 15 (8.7%), 19 (8.7%), and chromosome 13 (8.6%). Pathway analysis showed most genes to be involved in PDGF pathway, ubiquitin proteasome pathway, Ras pathway and TNFR1 signaling pathway. Although almost all chromosomes have been at least once affected by either gain or loss of genetic material, number of genes with affected exrrpession is relatively low. We anticipate two mechanism for expression level compensations: i) increase of related supressors activity in case of gains ii) impact of second allele in case of losses.

Návaznosti

NT11154, projekt VaV

Název: Úloha mitotické disrupce v B lymfocytech u mnohočetném myelomu

Investor: Ministerstvo zdravotnictví ČR, Úloha mitotické disrupce v B lymfocytech u mnohočetného myelomu

NT13190, projekt VaV

Název: Molekulární charakteristika centrozomálních abnormalit a jejich prognostický význam pro pacienty s mnohočetným myelomem

Investor: Ministerstvo zdravotnictví ČR, Molekulární charakteristika centrozomálních abnormalit a jejich prognostický význam pro pacienty s mnohočetným myelomem

Citovat

NĚMEC, Pavel, Fedor KRYUKOV, Elena Vladimirovna DEMENTYEVA, Jan SMETANA, Ivana IHNATOVÁ, Luděk POUR, Petr KUGLÍK a Roman HÁJEK. Effect of DNA Dosage into Gene Expression in Patients with Multiple Myeloma. In 14th International Myeloma Workshop. 2013. ISSN 2152-2650.

@proceedings{1107538,
   author = {Němec, Pavel and Kryukov, Fedor and Dementyeva, Elena Vladimirovna and Smetana, Jan and Ihnatová, Ivana and Pour, Luděk and Kuglík, Petr and Hájek, Roman},
   booktitle = {14th International Myeloma Workshop},
   language = {eng},
   title = {Effect of DNA Dosage into Gene Expression in Patients with Multiple Myeloma},
   year = {2013}
}

TY  - CONF
ID  - 1107538
AU  - Němec, Pavel - Kryukov, Fedor - Dementyeva, Elena Vladimirovna - Smetana, Jan - Ihnatová, Ivana - Pour, Luděk - Kuglík, Petr - Hájek, Roman
PY  - 2013
TI  - Effect of DNA Dosage into Gene Expression in Patients with Multiple Myeloma
N2  - Whole genomic methods represents effective tool for studying genomic changes in cancer cells. Aim of this study was to find and describe genes whose expression is dependent on the DNA copy number (gene dosage) in patients with multiple myeloma. Total of 57 patients with MM were simultaneously examined by arrayCGH for DNA copy number variations (gain/losses) utilizing Agilent Human Genome CGH Microarray 4x44K Arrays and for gene expression utilizing Affymetrix GeneChip Human Gene 1.0 ST. Gene dosage-dependent genes were defined by Spearman correlation[R>0.5, p(FDR)<0.05]of CNV status and expression level and analysed using DAVID Bioinformatics software. Total of 852 fromall 27391 transcripts were strongly and significantly dependent ongene dosage. Cytogeneticaly, majority (25%) of all 852 genes were located on chromosome 1 (with 19 genes mapped to 1q21 locus). Other involved genes were mostly located on chromosomes 15 (8.7%), 19 (8.7%), and chromosome 13 (8.6%). Pathway analysis showed most genes to be involved in PDGF pathway, ubiquitin proteasome pathway, Ras pathway and TNFR1 signaling pathway. Although almost all chromosomes have been at least once affected by either gain or loss of genetic material, number of genes with affected exrrpession is relatively low. We anticipate two mechanism for expression level compensations: i) increase of related supressors activity in case of gains ii) impact of second allele in case of losses.
ER  -

NĚMEC, Pavel, Fedor KRYUKOV, Elena Vladimirovna DEMENTYEVA, Jan SMETANA, Ivana IHNATOVÁ, Luděk POUR, Petr KUGLÍK a Roman HÁJEK. Effect of DNA Dosage into Gene Expression in Patients with Multiple Myeloma. In \textit{14th International Myeloma Workshop}. 2013. ISSN~2152-2650.

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