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@article{1107873,
author = {Šalplachta, Jiří and Kubesová, Anna and Moravcová, Dana and Vykydalová, Marie and Süle, Sándor and Matoušková, Hana and Horký, Jaroslav and Horká, Marie},
article_location = {Heidelberg},
article_number = {10},
doi = {http://dx.doi.org/10.1007/s00216-013-6754-z},
keywords = {DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; CAPILLARY-ELECTROPHORESIS; STAPHYLOCOCCUS-AUREUS; HELICOBACTER-PYLORI; CEREBROSPINAL-FLUID; ISOELECTRIC POINT; IDENTIFICATION; MICROORGANISMS; SEPARATION},
language = {eng},
issn = {1618-2642},
journal = {Analytical and Bioanalytical Chemistry},
title = {Use of electrophoretic techniques and MALDI–TOF MS for rapid and reliable characterization of bacteria: analysis of intact cells, cell lysates, and “washed pellets”},
url = {http://link.springer.com/article/10.1007%2Fs00216-013-6754-z#/page-1},
volume = {405},
year = {2013}
}
TY - JOUR
ID - 1107873
AU - Šalplachta, Jiří - Kubesová, Anna - Moravcová, Dana - Vykydalová, Marie - Süle, Sándor - Matoušková, Hana - Horký, Jaroslav - Horká, Marie
PY - 2013
TI - Use of electrophoretic techniques and MALDI–TOF MS for rapid and reliable characterization of bacteria: analysis of intact cells, cell lysates, and “washed pellets”
JF - Analytical and Bioanalytical Chemistry
VL - 405
IS - 10
SP - 3165-3175
EP - 3165-3175
PB - SPRINGER HEIDELBERG
SN - 16182642
KW - DESORPTION IONIZATION-TIME
KW - FLIGHT MASS-SPECTROMETRY
KW - CAPILLARY-ELECTROPHORESIS
KW - STAPHYLOCOCCUS-AUREUS
KW - HELICOBACTER-PYLORI
KW - CEREBROSPINAL-FLUID
KW - ISOELECTRIC POINT
KW - IDENTIFICATION
KW - MICROORGANISMS
KW - SEPARATION
UR - http://link.springer.com/article/10.1007%2Fs00216-013-6754-z#/page-1
L2 - http://link.springer.com/article/10.1007%2Fs00216-013-6754-z#/page-1
N2 - In this study electrophoretic and mass spectrometric analysis of three types of bacterial sample (intact cells, cell lysates, and “washed pellets”) were used to develop an effective procedure for the characterization of bacteria. The samples were prepared from specific bacterial strains. Five strains representing different species of the family Rhizobiaceae were selected as model microorganisms: Rhizobium leguminosarum bv. trifolii, R. leguminosarum bv. viciae, R. galegae, R. loti, and Sinorhizobium meliloti. Samples of bacteria were subjected to analysis by four techniques: capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), gel IEF, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS). These methods are potential alternatives to DNA-based methods for rapid and reliable characterization of bacteria. Capillary electrophoretic (CZE and CIEF) analysis of intact cells was suitable for characterization of different bacterial species. CIEF fingerprints of “washed pellets” and gel IEF of cell lysates helped to distinguish between closely related bacterial species that were not sufficiently differentiated by capillary electrophoretic analysis of intact cells. MALDI–TOF MS of “washed pellets” enabled more reliable characterization of bacteria than analysis of intact cells or cell lysates. Electrophoretic techniques and MALDI–TOF MS can both be successfully used to complement standard methods for rapid characterization of bacteria.
ER -
ŠALPLACHTA, Jiří, Anna KUBESOVÁ, Dana MORAVCOVÁ, Marie VYKYDALOVÁ, Sándor SÜLE, Hana MATOUŠKOVÁ, Jaroslav HORKÝ a Marie HORKÁ. Use of electrophoretic techniques and MALDI–TOF MS for rapid and reliable characterization of bacteria: analysis of intact cells, cell lysates, and “washed pellets”. \textit{Analytical and Bioanalytical Chemistry}. Heidelberg: SPRINGER HEIDELBERG, 2013, roč.~405, č.~10, s.~3165-3175. ISSN~1618-2642. Dostupné z: https://dx.doi.org/10.1007/s00216-013-6754-z.
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