Determination of antibiotics (tetracyclines and sulfonamides)
in biosolids by pressurized liquid extraction and liquid
chromatography-tandem mass spectrometry

J 2013

Determination of antibiotics (tetracyclines and sulfonamides) in biosolids by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry

PAMREDDY, Annapurna; M. HIDALGO; Josef HAVEL a Victoria SALVADO

Základní údaje

Originální název

Determination of antibiotics (tetracyclines and sulfonamides) in biosolids by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry

Autoři

PAMREDDY, Annapurna (356 Indie, domácí); M. HIDALGO (724 Španělsko); Josef HAVEL (203 Česká republika, domácí) a Victoria SALVADO (724 Španělsko)

Vydání

Journal of Chromatography A, AMSTERDAM, Elsevier, 2013, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.258

Kód RIV

RIV/00216224:14310/13:00080192

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.chroma.2013.05.014

UT WoS

000321174700009

Klíčová slova anglicky

Liquid chromatography-tandem mass spectrometry; Pressurized liquid extraction; Tetracyclines; Sulfonamides; Sludge; Wastewater treatment plants

Štítky

AKR, rivok

Změněno: 20. 5. 2015 14:08, Ing. Andrea Mikešková

Anotace

V originále

A robust and sensitive analytical method is developed to quantitatively determine tetracyclines and sulfonamides, two major antibiotic classes, in sewage sludge. The antibiotic agents, oxytetracycline, tetracycline, dioxycycline, chlorotetracycline, sulfathiazole, sulfapyridine, sulfamethazine and sulfamethoxazole, were extracted using pressurized liquid extraction (PLE) with citric acid at pH 3 and methanol (1:1 v/v). Clean-up of the extracts was performed by solid phase extraction (SPE) with hydrophilic-lipophilic balance cartridges. Identification and quantification of the compounds is by liquid chromatography-tandem mass spectrometry in multiple reaction monitoring (MRM) mode. High recoveries ranging from 90.4 to 99.9% for sulfonamides and 96.2 to 100.9% for the tetracyclines are obtained. Method detection limits (MDLs) range from 0.6 to 4.2 ng/g for sulfonamides and 3.2 to 13 ng/g for tetracyclines. After validation, the method is applied to the analysis of sludges collected from different WWTPs in Spain. (C) 2013 Elsevier B.V. All rights reserved.

Citovat

PAMREDDY, Annapurna; M. HIDALGO; Josef HAVEL a Victoria SALVADO. Determination of antibiotics (tetracyclines and sulfonamides) in biosolids by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A. AMSTERDAM: Elsevier, 2013, roč. 1298, july, s. 68-75. ISSN 0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2013.05.014.

@article{1107890,
   author = {Pamreddy, Annapurna and Hidalgo, M. and Havel, Josef and Salvado, Victoria},
   article_location = {AMSTERDAM},
   article_number = {july},
   doi = {https://doi.org/10.1016/j.chroma.2013.05.014},
   keywords = {Liquid chromatography-tandem mass spectrometry; Pressurized liquid extraction; Tetracyclines; Sulfonamides; Sludge; Wastewater treatment plants},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Determination of antibiotics (tetracyclines and sulfonamides) in biosolids by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry},
   url = {http://authors.elsevier.com/sd/article/S0021967313007644},
   volume = {1298},
   year = {2013}
}

TY  - JOUR
ID  - 1107890
AU  - Pamreddy, Annapurna - Hidalgo, M. - Havel, Josef - Salvado, Victoria
PY  - 2013
TI  - Determination of antibiotics (tetracyclines and sulfonamides) in biosolids by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry
JF  - Journal of Chromatography A
VL  - 1298
IS  - july
SP  - 68-75
EP  - 68-75
PB  - Elsevier
SN  - 00219673
KW  - Liquid chromatography-tandem mass spectrometry
KW  - Pressurized liquid extraction
KW  - Tetracyclines
KW  - Sulfonamides
KW  - Sludge
KW  - Wastewater treatment plants
UR  - http://authors.elsevier.com/sd/article/S0021967313007644
N2  - A robust and sensitive analytical method is developed to quantitatively determine tetracyclines and sulfonamides, two major antibiotic classes, in sewage sludge. The antibiotic agents, oxytetracycline, tetracycline, dioxycycline, chlorotetracycline, sulfathiazole, sulfapyridine, sulfamethazine and sulfamethoxazole, were extracted using pressurized liquid extraction (PLE) with citric acid at pH 3 and methanol (1:1 v/v). Clean-up of the extracts was performed by solid phase extraction (SPE) with hydrophilic-lipophilic balance cartridges. Identification and quantification of the compounds is by liquid chromatography-tandem mass spectrometry in multiple reaction monitoring (MRM) mode. High recoveries ranging from 90.4 to 99.9% for sulfonamides and 96.2 to 100.9% for the tetracyclines are obtained. Method detection limits (MDLs) range from 0.6 to 4.2 ng/g for sulfonamides and 3.2 to 13 ng/g for tetracyclines. After validation, the method is applied to the analysis of sludges collected from different WWTPs in Spain. (C) 2013 Elsevier B.V. All rights reserved.
ER  -

PAMREDDY, Annapurna; M. HIDALGO; Josef HAVEL a Victoria SALVADO. Determination of antibiotics (tetracyclines and sulfonamides) in biosolids by pressurized liquid extraction and liquid chromatography-tandem mass spectrometry. \textit{Journal of Chromatography A}. AMSTERDAM: Elsevier, 2013, roč.~1298, july, s.~68-75. ISSN~0021-9673. Dostupné z: https://doi.org/10.1016/j.chroma.2013.05.014.

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