BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA

D 2013

BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA

NEJDL, Lukáš; Branislav RUTTKAY-NEDECKÝ; Jiří KUDR; Kristýna ŠMERKOVÁ; Simona DOSTÁLOVÁ et al.

Základní údaje

Originální název

BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA

Autoři

NEJDL, Lukáš; Branislav RUTTKAY-NEDECKÝ; Jiří KUDR; Kristýna ŠMERKOVÁ; Simona DOSTÁLOVÁ; Pavel KOPEL; Josef ZEHNÁLEK; Libuše TRNKOVÁ; Vojtěch ADAM a René KIZEK

Vydání

Brno, XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, sborník, od s. 66-67, 2 s. 2013

Nakladatel

Mendelova univerzita v Brně

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10403 Physical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00068522

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-7375-757-1

Klíčová slova anglicky

DNA;zinc;UV/VIS spectrophotometry

Změněno: 10. 6. 2013 13:04, Mgr. Sylvie Dohnalíková

Anotace

V originále

We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ions for 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease in the M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II). On the other hand, application of two concentrations, 44 and 55 microM Zn(II), caused no changes in Tm. Gel electrophoresis proved significance of Zn(II) ions in the DNA renaturation. Samples of M-DNA (5microg/ml DNA + 5.5-55microM Zn(II) showed a significant changes in the renaturation in comparison with control (5microg/ml DNA). This study has proved that the zinc ion can incorporate into DNA (M-DNA) at standard conditions (25 C). This work suggests that the new structure of DNA (M-DNA) may occur in living organisms.

Citovat

NEJDL, Lukáš; Branislav RUTTKAY-NEDECKÝ; Jiří KUDR; Kristýna ŠMERKOVÁ; Simona DOSTÁLOVÁ; Pavel KOPEL; Josef ZEHNÁLEK; Libuše TRNKOVÁ; Vojtěch ADAM a René KIZEK. BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA. In XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, sborník. Brno: Mendelova univerzita v Brně, 2013, s. 66-67. ISBN 978-80-7375-757-1.

@inproceedings{1110913,
   author = {Nejdl, Lukáš and RuttkayandNedecký, Branislav and Kudr, Jiří and Šmerková, Kristýna and Dostálová, Simona and Kopel, Pavel and Zehnálek, Josef and Trnková, Libuše and Adam, Vojtěch and Kizek, René},
   address = {Brno},
   booktitle = {XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, sborník},
   keywords = {DNA;zinc;UV/VIS spectrophotometry},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-7375-757-1},
   pages = {66-67},
   publisher = {Mendelova univerzita v Brně},
   title = {BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA},
   year = {2013}
}

TY  - CONF
ID  - 1110913
AU  - Nejdl, Lukáš - Ruttkay-Nedecký, Branislav - Kudr, Jiří - Šmerková, Kristýna - Dostálová, Simona - Kopel, Pavel - Zehnálek, Josef - Trnková, Libuše - Adam, Vojtěch - Kizek, René
PY  - 2013
TI  - BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA
PB  - Mendelova univerzita v Brně
CY  - Brno
SN  - 9788073757571
KW  - DNA;zinc;UV/VIS spectrophotometry
N2  - We focused interactions of zinc ions and DNA in this study. Interactions were monitored using UV/VIS spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified fragment of DNA of 498 bp. DNA was subsequently incubated with Zn(II) ions for 60 min at 25 C. After incubation, samples were dialyzed and analysed immediately. In this way, DNA was converted into a metal bound DNA (M-DNA). Interaction of Zn(II) with DNA showed a change of the absorption spectrum (190-350 nm) and a decrease in the M-DNA melting temperature. Spectrophotometric (UV/VIS) analysis showed that increasing concentrations of Zn(II) led to an increase in absorbance at 200 nm and a decrease in absorbance at 260 nm. Application of 5.5 microM Zn(II) caused a decrease in the melting temperature (Tm) for about 7.5 C in average in comparison with control (75.5 +- 3 C). The lowest melting temperature (60.5 +- 2.5 C) was observed after application of 33 microM Zn(II). On the other hand, application of two concentrations, 44 and 55 microM Zn(II), caused no changes in Tm. Gel electrophoresis proved significance of Zn(II) ions in the DNA renaturation. Samples of M-DNA (5microg/ml DNA + 5.5-55microM Zn(II) showed a significant changes in the renaturation in comparison with control (5microg/ml DNA). This study has proved that the zinc ion can incorporate into DNA (M-DNA) at standard conditions (25 C). This work suggests that the new structure of DNA (M-DNA) may occur in living organisms.
ER  -

NEJDL, Lukáš; Branislav RUTTKAY-NEDECKÝ; Jiří KUDR; Kristýna ŠMERKOVÁ; Simona DOSTÁLOVÁ; Pavel KOPEL; Josef ZEHNÁLEK; Libuše TRNKOVÁ; Vojtěch ADAM a René KIZEK. BIOPHYSICAL STUDY OF THE INTERACTION OF ZINC IONS WITH DNA. In \textit{XIII. Pracovní setkání fyzikálních chemiků a elektrochemiků, sborník}. Brno: Mendelova univerzita v Brně, 2013, s.~66-67. ISBN~978-80-7375-757-1.

Přehled o publikaci