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@inproceedings{1113171,
author = {Vojtek, Libor and Dobeš, Pavel and Büyükgüzel, Ender and Hyršl, Pavel},
address = {Darmstadt},
booktitle = {Insect pathogens and entomoparasitic nematodes},
keywords = {antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Darmstadt},
isbn = {978-92-9067-268-5},
pages = {333-338},
publisher = {IOBC-WPRS 2013},
title = {Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph},
year = {2013}
}
TY - JOUR
ID - 1113171
AU - Vojtek, Libor - Dobeš, Pavel - Büyükgüzel, Ender - Hyršl, Pavel
PY - 2013
TI - Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
PB - IOBC-WPRS 2013
CY - Darmstadt
SN - 9789290672685
KW - antibacterial activity
KW - Galleria mellonella
KW - Bombyx mori
KW - bioluminescent bacteria
N2 - We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
ER -
VOJTEK, Libor, Pavel DOBEŠ, Ender BÜYÜKGÜZEL a Pavel HYRŠL. Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph. In \textit{Insect pathogens and entomoparasitic nematodes}. Darmstadt: IOBC-WPRS 2013, 2013, s.~333-338. ISBN~978-92-9067-268-5.
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