2013
Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
VOJTEK, Libor, Pavel DOBEŠ, Ender BÜYÜKGÜZEL a Pavel HYRŠLZákladní údaje
Originální název
Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
Název česky
Bioluminescence determination of antibacterial activity of Bombyx mori and Galleria mellonella haemolymph
Autoři
VOJTEK, Libor (203 Česká republika, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Ender BÜYÜKGÜZEL (792 Turecko) a Pavel HYRŠL (203 Česká republika, garant, domácí)
Vydání
Darmstadt, Insect pathogens and entomoparasitic nematodes, od s. 333-338, 6 s. 2013
Nakladatel
IOBC-WPRS 2013
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
30102 Immunology
Stát vydavatele
Chorvatsko
Utajení
není předmětem státního či obchodního tajemství
Forma vydání
tištěná verze "print"
Kód RIV
RIV/00216224:14310/13:00065681
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-92-9067-268-5
Klíčová slova česky
antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria
Klíčová slova anglicky
antibacterial activity; Galleria mellonella; Bombyx mori; bioluminescent bacteria
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 30. 1. 2016 23:25, Mgr. Pavel Dobeš, Ph.D.
V originále
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
Česky
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria Photorhabdus luminescens and transformed Escherichia coli, which can be used for a real-time measurement of antibacterial activity. We observed a significant dose-dependent decrease of bioluminescence using both bacterial species during one hour after exposure to Bombyx mori or Galleria mellonella haemolymph. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed in haemolymph plasma without haemocytes. Antibacterial activity operating against Gram negative bacteria was measured in unaffected insect larvae as well as after septic injury; increased antibacterial activity in haemolymph was detected in the latter case which confirms inducibility of antimicrobial agents. This method can be widely used for determination of antibacterial activity in insects and supposedly in other invertebrates.
Návaznosti
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