The Delta Subunit of RNA Polymerase Is Required for Rapid
Changes in Gene Expression and Competitive Fitness of the Cell

J 2013

The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell

RABATINOVÁ, Alžbeta; Hana ŠANDEROVÁ; Jitka JIRÁT MATĚJČKOVÁ; Jana KORELUSOVÁ; Luděk SOJKA et al.

Základní údaje

Originální název

The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell

Autoři

RABATINOVÁ, Alžbeta; Hana ŠANDEROVÁ; Jitka JIRÁT MATĚJČKOVÁ; Jana KORELUSOVÁ; Luděk SOJKA; Ivan BARVÍK; Veronika PAPOUŠKOVÁ; Vladimír SKLENÁŘ; Lukáš ŽÍDEK a Libor KRÁSNÝ

Vydání

Journal of Bacteriology, Washington, American Society for Microbiology, 2013, 0021-9193

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.688

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/13:00066221

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1128/JB.00188-13

UT WoS

000318811300017

Klíčová slova anglicky

RNA polymerase; delta subunit; regulation; nucleoside triphosphate; gene expression; cell survival

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2014 14:11, Olga Křížová

Anotace

V originále

RNA polymerase (RNAP) is an extensively studied multisubunit enzyme required for transcription of DNA into RNA, yet the delta subunit of RNAP remains an enigmatic protein whose physiological roles have not been fully elucidated. Here, we identify a novel, so far unrecognized function of delta from Bacillus subtilis. We demonstrate that delta affects the regulation of RNAP by the concentration of the initiating nucleoside triphosphate ([iNTP]), an important mechanism crucial for rapid changes in gene expression in response to environmental changes. Consequently, we demonstrate that delta is essential for cell survival when facing a competing strain in a changing environment. Hence, although delta is not essential per se, it is vital for the cell's ability to rapidly adapt and survive in nature. Finally, we show that two other proteins, GreA and YdeB, previously implicated to affect regulation of RNAP by [iNTP] in other organisms, do not have this function in B. subtilis.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA204/09/0583, projekt VaV

Název: Delta podjednotka RNA polymerázy z Gram pozitivních bakterií (Akronym: DELTA)

Investor: Grantová agentura ČR, Delta podjednotka RNA polymerázy z Gram pozitivních bakterií

Citovat

RABATINOVÁ, Alžbeta; Hana ŠANDEROVÁ; Jitka JIRÁT MATĚJČKOVÁ; Jana KORELUSOVÁ; Luděk SOJKA; Ivan BARVÍK; Veronika PAPOUŠKOVÁ; Vladimír SKLENÁŘ; Lukáš ŽÍDEK a Libor KRÁSNÝ. The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell. Journal of Bacteriology. Washington: American Society for Microbiology, 2013, roč. 195, č. 11, s. 2603-2611. ISSN 0021-9193. Dostupné z: https://doi.org/10.1128/JB.00188-13.

@article{1113356,
   author = {Rabatinová, Alžbeta and Šanderová, Hana and Jirát Matějčková, Jitka and Korelusová, Jana and Sojka, Luděk and Barvík, Ivan and Papoušková, Veronika and Sklenář, Vladimír and Žídek, Lukáš and Krásný, Libor},
   article_location = {Washington},
   article_number = {11},
   doi = {https://doi.org/10.1128/JB.00188-13},
   keywords = {RNA polymerase; delta subunit; regulation; nucleoside triphosphate; gene expression; cell survival},
   language = {eng},
   issn = {0021-9193},
   journal = {Journal of Bacteriology},
   title = {The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell},
   url = {http://jb.asm.org/content/195/11/2603.full},
   volume = {195},
   year = {2013}
}

TY  - JOUR
ID  - 1113356
AU  - Rabatinová, Alžbeta - Šanderová, Hana - Jirát Matějčková, Jitka - Korelusová, Jana - Sojka, Luděk - Barvík, Ivan - Papoušková, Veronika - Sklenář, Vladimír - Žídek, Lukáš - Krásný, Libor
PY  - 2013
TI  - The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell
JF  - Journal of Bacteriology
VL  - 195
IS  - 11
SP  - 2603-2611
EP  - 2603-2611
PB  - American Society for Microbiology
SN  - 00219193
KW  - RNA polymerase
KW  - delta subunit
KW  - regulation
KW  - nucleoside triphosphate
KW  - gene expression
KW  - cell survival
UR  - http://jb.asm.org/content/195/11/2603.full
L2  - http://jb.asm.org/content/195/11/2603.full
N2  - RNA polymerase (RNAP) is an extensively studied multisubunit enzyme required for transcription of DNA into RNA, yet the delta subunit of RNAP remains an enigmatic protein whose physiological roles have not been fully elucidated. Here, we identify a novel, so far unrecognized function of delta from Bacillus subtilis. We demonstrate that delta affects the regulation of RNAP by the concentration of the initiating nucleoside triphosphate ([iNTP]), an important mechanism crucial for rapid changes in gene expression in response to environmental changes. Consequently, we demonstrate that delta is essential for cell survival when facing a competing strain in a changing environment. Hence, although delta is not essential per se, it is vital for the cell's ability to rapidly adapt and survive in nature. Finally, we show that two other proteins, GreA and YdeB, previously implicated to affect regulation of RNAP by [iNTP] in other organisms, do not have this function in B. subtilis.
ER  -

RABATINOVÁ, Alžbeta; Hana ŠANDEROVÁ; Jitka JIRÁT MATĚJČKOVÁ; Jana KORELUSOVÁ; Luděk SOJKA; Ivan BARVÍK; Veronika PAPOUŠKOVÁ; Vladimír SKLENÁŘ; Lukáš ŽÍDEK a Libor KRÁSNÝ. The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell. \textit{Journal of Bacteriology}. Washington: American Society for Microbiology, 2013, roč.~195, č.~11, s.~2603-2611. ISSN~0021-9193. Dostupné z: https://doi.org/10.1128/JB.00188-13.

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