HYRŠL, Pavel, Pavel DOBEŠ, Badrul AREFIN, Lucie KUČEROVÁ, Robert MARKUS, Zhi WANG, Michal ŽUROVEC a Ulrich THEOPOLD. New insights to insect response to the infection by nematobacterial complex. In 14th Meeting of the IOBS/wprs Insect pathogens and entomoparasitic nematodes. 2013. ISBN 978-92-9067-268-5.
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Základní údaje
Originální název New insights to insect response to the infection by nematobacterial complex
Autoři HYRŠL, Pavel (203 Česká republika, garant, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Badrul AREFIN (50 Bangladéš), Lucie KUČEROVÁ (203 Česká republika), Robert MARKUS (348 Maďarsko), Zhi WANG (752 Švédsko), Michal ŽUROVEC (203 Česká republika) a Ulrich THEOPOLD (752 Švédsko).
Vydání 14th Meeting of the IOBS/wprs Insect pathogens and entomoparasitic nematodes, 2013.
Další údaje
Originální jazyk angličtina
Typ výsledku Konferenční abstrakt
Obor Immunology
Stát vydavatele Chorvatsko
Utajení není předmětem státního či obchodního tajemství
Kód RIV RIV/00216224:14310/13:00065683
Organizační jednotka Přírodovědecká fakulta
ISBN 978-92-9067-268-5
Klíčová slova česky Drosophila melanogaster; entomopatogenní hlístovky; genomová analýza; imunita; infekce; symbiotické bakterie
Klíčová slova anglicky Drosophila melanogaster; entomopathogenic nematodes; genome-wide analysis; immunity; infection; symbiotic bacteria
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: doc. RNDr. Pavel Hyršl, Ph.D., učo 9982. Změněno: 20. 10. 2013 21:42.
Anotace
Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves as well for expanding nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system we used before to show the importance of haemolymph clotting and eicosanoids during the infection. We used the well-established tripartite model (Drosophila, nematodes, bacteria), DNA chips and bioinformatic tools to compare gene expression in non-infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. Infected (GFP positive) larvae were collected 6 hours after infection. We detected approximately 650 genes whose expression was significantly influenced by nematobacterial infection caused by H. bacteriophora and P. luminescens. Most of them are upregulated upon infection including mainly the genes involved in antimicrobial response and development. Based on Gene Ontology annotation we identified several pathways, which could be involved in sealing and repairing the wound caused by invading nematodes. These results we compared with available data for other types infection caused by bacteria and parasitic wasps. Small group of genes were common for all three types of infection and approximately 25 genes were overlapping in each pairwise comparison. We focused on the genes expressed in the hemocytes and fat body, respectively and we subjected selected candidate genes to functional tests. We tested the effect of mutations or knockdown of selected genes for the susceptibility of flies to the nematobacterial infection. The overlap between the protective genes and genes induced by the nematobacterial infection was not complete. Therefore, we assume that only a fraction of the genes involved in the protection of infected larvae from death are induced by the nematobacterial infection. Our research is supported by research grants from the Swedish Research Council (VR-NT 2010-5118), the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) and by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047).
Návaznosti
CZ.1.07/2.3.00/30.0009, interní kód MU
(Kód CEP: EE2.3.30.0009)
Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci (Akronym: Postdoc I.)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Operační program Vzdělávání pro konkurenceschopnost (VK), 2.3 Lidské zdroje ve výzkumu a vývoji
QJ1210047, projekt VaVNázev: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění (Akronym: Probiotika - imunita včel)
Investor: Ministerstvo zemědělství ČR, KUS - Komplexní udržitelné systémy v zemědělství 2012-2018
VytisknoutZobrazeno: 24. 8. 2019 04:41