New insights to insect response to the infection by
nematobacterial complex

a 2013

New insights to insect response to the infection by nematobacterial complex

HYRŠL, Pavel; Pavel DOBEŠ; Badrul AREFIN; Lucie KUČEROVÁ; Robert MARKUS et al.

Základní údaje

Originální název

New insights to insect response to the infection by nematobacterial complex

Autoři

HYRŠL, Pavel; Pavel DOBEŠ; Badrul AREFIN; Lucie KUČEROVÁ; Robert MARKUS; Zhi WANG; Michal ŽUROVEC a Ulrich THEOPOLD

Vydání

14th Meeting of the IOBS/wprs Insect pathogens and entomoparasitic nematodes, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30102 Immunology

Stát vydavatele

Chorvatsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/13:00065683

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-92-9067-268-5

Klíčová slova česky

Drosophila melanogaster; entomopatogenní hlístovky; genomová analýza; imunita; infekce; symbiotické bakterie

Klíčová slova anglicky

Drosophila melanogaster; entomopathogenic nematodes; genome-wide analysis; immunity; infection; symbiotic bacteria

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 10. 2013 21:42, doc. RNDr. Pavel Hyršl, Ph.D.

Anotace

V originále

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves as well for expanding nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system we used before to show the importance of haemolymph clotting and eicosanoids during the infection. We used the well-established tripartite model (Drosophila, nematodes, bacteria), DNA chips and bioinformatic tools to compare gene expression in non-infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. Infected (GFP positive) larvae were collected 6 hours after infection. We detected approximately 650 genes whose expression was significantly influenced by nematobacterial infection caused by H. bacteriophora and P. luminescens. Most of them are upregulated upon infection including mainly the genes involved in antimicrobial response and development. Based on Gene Ontology annotation we identified several pathways, which could be involved in sealing and repairing the wound caused by invading nematodes. These results we compared with available data for other types infection caused by bacteria and parasitic wasps. Small group of genes were common for all three types of infection and approximately 25 genes were overlapping in each pairwise comparison. We focused on the genes expressed in the hemocytes and fat body, respectively and we subjected selected candidate genes to functional tests. We tested the effect of mutations or knockdown of selected genes for the susceptibility of flies to the nematobacterial infection. The overlap between the protective genes and genes induced by the nematobacterial infection was not complete. Therefore, we assume that only a fraction of the genes involved in the protection of infected larvae from death are induced by the nematobacterial infection. Our research is supported by research grants from the Swedish Research Council (VR-NT 2010-5118), the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) and by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047).

Návaznosti

CZ.1.07/2.3.00/30.0009, interní kód MU
(Kód CEP: EE2.3.30.0009)

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci (Akronym: Postdoc I.)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci, 2.3 Lidské zdroje ve výzkumu a vývoji

QJ1210047, projekt VaV

Název: Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění (Akronym: Probiotika - imunita včel)

Investor: Ministerstvo zemědělství ČR, Vývoj nových prostředků pro podporu imunity včel, prevenci a léčbu včelích onemocnění

Citovat

HYRŠL, Pavel; Pavel DOBEŠ; Badrul AREFIN; Lucie KUČEROVÁ; Robert MARKUS; Zhi WANG; Michal ŽUROVEC a Ulrich THEOPOLD. New insights to insect response to the infection by nematobacterial complex. In 14th Meeting of the IOBS/wprs Insect pathogens and entomoparasitic nematodes. 2013. ISBN 978-92-9067-268-5.

@proceedings{1114271,
   author = {Hyršl, Pavel and Dobeš, Pavel and Arefin, Badrul and Kučerová, Lucie and Markus, Robert and Wang, Zhi and Žurovec, Michal and Theopold, Ulrich},
   booktitle = {14th Meeting of the IOBS/wprs Insect pathogens and entomoparasitic nematodes},
   keywords = {Drosophila melanogaster; entomopathogenic nematodes; genome-wide analysis; immunity; infection; symbiotic bacteria},
   language = {eng},
   isbn = {978-92-9067-268-5},
   title = {New insights to insect response to the infection by nematobacterial complex},
   year = {2013}
}

TY  - CONF
ID  - 1114271
AU  - Hyršl, Pavel - Dobeš, Pavel - Arefin, Badrul - Kučerová, Lucie - Markus, Robert - Wang, Zhi - Žurovec, Michal - Theopold, Ulrich
PY  - 2013
TI  - New insights to insect response to the infection by nematobacterial complex
SN  - 9789290672685
KW  - Drosophila melanogaster
KW  - entomopathogenic nematodes
KW  - genome-wide analysis
KW  - immunity
KW  - infection
KW  - symbiotic bacteria
N2  - Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis are obligate and lethal insect parasites. In recent years they have been used increasingly as biological control agents. These EPNs are symbiotically associated with bacteria of the genera Photorhabdus. The bacterial symbionts are essential to kill the host (within 24-48 hours) and digest its tissues to provide nutrients for themselves as well for expanding nematodes. Drosophila larvae are suitable insect hosts and part of the tripartite model system we used before to show the importance of haemolymph clotting and eicosanoids during the infection. We used the well-established tripartite model (Drosophila, nematodes, bacteria), DNA chips and bioinformatic tools to compare gene expression in non-infected and infected fly larvae. We focused on the early time point of nematode infection and therefore infected Drosophila larvae using H. bacteriophora harbouring GFP-labelled P. luminescens bacteria. Infected (GFP positive) larvae were collected 6 hours after infection. We detected approximately 650 genes whose expression was significantly influenced by nematobacterial infection caused by H. bacteriophora and P. luminescens. Most of them are upregulated upon infection including mainly the genes involved in antimicrobial response and development. Based on Gene Ontology annotation we identified several pathways, which could be involved in sealing and repairing the wound caused by invading nematodes. These results we compared with available data for other types infection caused by bacteria and parasitic wasps. Small group of genes were common for all three types of infection and approximately 25 genes were overlapping in each pairwise comparison. We focused on the genes expressed in the hemocytes and fat body, respectively and we subjected selected candidate genes to functional tests. We tested the effect of mutations or knockdown of selected genes for the susceptibility of flies to the nematobacterial infection. The overlap between the protective genes and genes induced by the nematobacterial infection was not complete. Therefore, we assume that only a fraction of the genes involved in the protection of infected larvae from death are induced by the nematobacterial infection. Our research is supported by research grants from the Swedish Research Council (VR-NT 2010-5118), the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) and by grant from Ministry of Agriculture of Czech Republic (NAZV-KUS QJ1210047).
ER  -

HYRŠL, Pavel; Pavel DOBEŠ; Badrul AREFIN; Lucie KUČEROVÁ; Robert MARKUS; Zhi WANG; Michal ŽUROVEC a Ulrich THEOPOLD. New insights to insect response to the infection by nematobacterial complex. In \textit{14th Meeting of the IOBS/wprs Insect pathogens and entomoparasitic nematodes}. 2013. ISBN~978-92-9067-268-5.

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