Structure of rrn operons in pathogenic noncultivable
treponemes: sequence but not genomic position of intergenic
spacers correlates with classification of Treponema pallidum
and Treponema paraluiscuniculi strains

J 2013

Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains

ČEJKOVÁ, Darina; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; Michal STROUHAL; Lenka MIKALOVÁ et al.

Základní údaje

Originální název

Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains

Autoři

ČEJKOVÁ, Darina; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; Michal STROUHAL; Lenka MIKALOVÁ ; George M. WEINSTOCK a David ŠMAJS

Vydání

Journal of Medical Microbiology, Reading, Society for General Microbiology, 2013, 0022-2615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.266

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/13:00065613

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1099/jmm.0.050658-0

UT WoS

000315554000004

Klíčová slova anglicky

article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 4. 2014 16:24, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.

Návaznosti

GAP302/12/0574, projekt VaV

Název: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Grantová agentura ČR, Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

NT11159, projekt VaV

Název: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministerstvo zdravotnictví ČR, Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Přiložené soubory

cejkova.pdf

Požádat o autorskou verzi souboru

Citovat

ČEJKOVÁ, Darina; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; Michal STROUHAL; Lenka MIKALOVÁ; George M. WEINSTOCK a David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. Journal of Medical Microbiology. Reading: Society for General Microbiology, 2013, roč. 62, č. 2, s. 196-207. ISSN 0022-2615. Dostupné z: https://doi.org/10.1099/jmm.0.050658-0.

@article{1115115,
   author = {Čejková, Darina and Zobaníková, Marie and Pospíšilová, Petra and Strouhal, Michal and Mikalová, Lenka and Weinstock, George M. and Šmajs, David},
   article_location = {Reading},
   article_number = {2},
   doi = {https://doi.org/10.1099/jmm.0.050658-0},
   keywords = {article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi},
   language = {eng},
   issn = {0022-2615},
   journal = {Journal of Medical Microbiology},
   title = {Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains},
   volume = {62},
   year = {2013}
}

TY  - JOUR
ID  - 1115115
AU  - Čejková, Darina - Zobaníková, Marie - Pospíšilová, Petra - Strouhal, Michal - Mikalová, Lenka - Weinstock, George M. - Šmajs, David
PY  - 2013
TI  - Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
JF  - Journal of Medical Microbiology
VL  - 62
IS  - 2
SP  - 196-207
EP  - 196-207
PB  - Society for General Microbiology
SN  - 00222615
KW  - article
KW  - bacterial strain
KW  - bacterium isolate
KW  - controlled study
KW  - gene deletion
KW  - genotype
KW  - nonhuman
KW  - operon
KW  - polymerase chain reaction
KW  - priority journal
KW  - RNA sequence
KW  - Treponema
KW  - Treponema pallidum
KW  - Treponema paraluiscuniculi
N2  - This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
ER  -

ČEJKOVÁ, Darina; Marie ZOBANÍKOVÁ; Petra POSPÍŠILOVÁ; Michal STROUHAL; Lenka MIKALOVÁ; George M. WEINSTOCK a David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. \textit{Journal of Medical Microbiology}. Reading: Society for General Microbiology, 2013, roč.~62, č.~2, s.~196-207. ISSN~0022-2615. Dostupné z: https://doi.org/10.1099/jmm.0.050658-0.

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