Structure of rrn operons in pathogenic noncultivable
treponemes: sequence but not genomic position of intergenic
spacers correlates with classification of Treponema pallidum
and Treponema paraluiscuniculi strains

J 2013

Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ et. al.

Základní údaje

Originální název

Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains

Autoři

ČEJKOVÁ, Darina (203 Česká republika, domácí), Marie ZOBANÍKOVÁ (203 Česká republika, domácí), Petra POSPÍŠILOVÁ (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Lenka MIKALOVÁ (203 Česká republika, domácí), George M. WEINSTOCK (840 Spojené státy) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

Journal of Medical Microbiology, Reading, Society for General Microbiology, 2013, 0022-2615

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.266

Kód RIV

RIV/00216224:14110/13:00065613

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1099/jmm.0.050658-0

UT WoS

000315554000004

Klíčová slova anglicky

article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 4. 2014 16:24, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.

Návaznosti

GAP302/12/0574, projekt VaV

Název: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Grantová agentura ČR, Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

NT11159, projekt VaV

Název: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministerstvo zdravotnictví ČR, Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Přiložené soubory

cejkova.pdf

Požádat o autorskou verzi souboru

Citovat

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ, George M. WEINSTOCK a David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. Journal of Medical Microbiology. Reading: Society for General Microbiology, 2013, roč. 62, č. 2, s. 196-207. ISSN 0022-2615. Dostupné z: https://dx.doi.org/10.1099/jmm.0.050658-0.

@article{1115115,
   author = {Čejková, Darina and Zobaníková, Marie and Pospíšilová, Petra and Strouhal, Michal and Mikalová, Lenka and Weinstock, George M. and Šmajs, David},
   article_location = {Reading},
   article_number = {2},
   doi = {http://dx.doi.org/10.1099/jmm.0.050658-0},
   keywords = {article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi},
   language = {eng},
   issn = {0022-2615},
   journal = {Journal of Medical Microbiology},
   title = {Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains},
   volume = {62},
   year = {2013}
}

TY  - JOUR
ID  - 1115115
AU  - Čejková, Darina - Zobaníková, Marie - Pospíšilová, Petra - Strouhal, Michal - Mikalová, Lenka - Weinstock, George M. - Šmajs, David
PY  - 2013
TI  - Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
JF  - Journal of Medical Microbiology
VL  - 62
IS  - 2
SP  - 196-207
EP  - 196-207
PB  - Society for General Microbiology
SN  - 00222615
KW  - article
KW  - bacterial strain
KW  - bacterium isolate
KW  - controlled study
KW  - gene deletion
KW  - genotype
KW  - nonhuman
KW  - operon
KW  - polymerase chain reaction
KW  - priority journal
KW  - RNA sequence
KW  - Treponema
KW  - Treponema pallidum
KW  - Treponema paraluiscuniculi
N2  - This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
ER  -

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ, George M. WEINSTOCK a David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. \textit{Journal of Medical Microbiology}. Reading: Society for General Microbiology, 2013, roč.~62, č.~2, s.~196-207. ISSN~0022-2615. Dostupné z: https://dx.doi.org/10.1099/jmm.0.050658-0.

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