a 2013

Apolipoprotein E gene polymorphisms in chronic periodontitis

BOŘILOVÁ LINHARTOVÁ, Petra, Jan MÁCHAL, Jiřina BÁRTOVÁ, Hana POSKEROVÁ, Jan VOKURKA et. al.

Basic information

Original name

Apolipoprotein E gene polymorphisms in chronic periodontitis

Authors

BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, belonging to the institution), Jan MÁCHAL (203 Czech Republic, belonging to the institution), Jiřina BÁRTOVÁ (203 Czech Republic, belonging to the institution), Hana POSKEROVÁ (203 Czech Republic, belonging to the institution), Jan VOKURKA (203 Czech Republic, belonging to the institution), Antonín FASSMANN (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457. 2013

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.986

RIV identification code

RIV/00216224:14110/13:00065618

Organization unit

Faculty of Medicine

ISSN

UT WoS

000325919202433

Keywords in English

ApoE; chronic periodontitis; gene polymorphism; microbial colonization

Abstract

V originále

Objective: Periodontal diseases can be related to common systemic conditions, and, as recently described, alterations in blood lipid levels. The aim of this study was to analyze two apolipoprotein E (ApoE) gene polymorphisms in patients with chronic periodontitis (CP) in relation to periodontopathic bacteria and lipid levels. Methods: 469 unrelated subjects were included in this case-control study. Genomic DNA of 294 patients with CP and 175 healthy/non-periodontitis controls were genotyped, using the real-time polymerase chain reaction (RT-PCR) method monitored by SYBR Green, for ApoE (rs429358 and rs7412) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit and lipid levels were measured in a subgroup of subjects. Results: There was no evidence for a significant association between ApoE gene polymorphisms and CP (E2/E2 vs. E3/E3: OR=0.63, 95%CI=0.12-3.15; E2/E3 vs. E3/E3: OR=1.61, 95%CI=0.84-3.11; E2/E4 vs. E3/E3: OR=1.25, 95%CI=0.23-6.95; E3/E4 vs. E3/E3: OR=1.11, 95%CI=0.65-1.88; E4/E4 vs. E3/E3: OR=0.63, 95%CI=0.15-2.55; allele E2 vs. allele E3: OR=1.26, 95%CI=0.74-2.14; allele E4 vs. allele E3: OR=0.97, 95%CI=0.63-1.51). Patients with CP had increased total cholesterol and LDL levels compared to controls (P<0.05), however no significant differences were found for triglyceride and HDL levels. Gene variability in the ApoE gene influenced LDL levels marginally (P=0.07) but did not modify total cholesterol, triglyceride and HDL levels or the occurrence of periodontal pathogens in subgingival pockets. Conclusions: In Czech population studied, ApoE genetic variations rs429358 and rs7412 were not associated with susceptibility to and severity of chronic periodontitis or with the presence of periodontopathic bacteria.

Links

MUNI/A/0888/2012, interní kód MU
Name: Výzkum diagnostických a terapeutických přístupů v predikci onemocnění v orofaciální oblasti
Investor: Masaryk University, Category A
NT11405, research and development project
Name: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu
Investor: Ministry of Health of the CR