Chicken innate immune response to oral infection with
Salmonella enterica serovar Enteritidis

J 2013

Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis

ELSHEIMER MATULOVÁ, Marta; Karolína VARMUŽOVÁ; František SISAK; Hana HAVÍČKOVÁ; Vladimír BABÁK et al.

Základní údaje

Originální název

Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis

Autoři

ELSHEIMER MATULOVÁ, Marta; Karolína VARMUŽOVÁ; František SISAK; Hana HAVÍČKOVÁ; Vladimír BABÁK; Karel STEJSKAL; Zbyněk ZDRÁHAL a Ivan RYCHLIK

Vydání

VETERINARY RESEARCH, LONDON, BIOMED CENTRAL LTD, 2013, 0928-4249

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.383

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/13:00066269

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1186/1297-9716-44-37

UT WoS

000319449200001

Klíčová slova anglicky

HEMATOPOIETIC LINEAGE; HS1 PROTEIN; CD8(+) T; TYPHIMURIUM; IDENTIFICATION; EXPANSION; SUBSTRATE; CELLS; GUT

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 1. 2014 11:59, Olga Křížová

Anotace

V originále

The characterization of the immune response of chickens to Salmonella infection is usually limited to the quantification of expression of genes coding for cytokines, chemokines or antimicrobial peptides. However, processes occurring in the cecum of infected chickens are likely to be much more diverse. In this study we have therefore characterized the transcriptome and proteome in the chicken cecum after infection with Salmonella Enteritidis. Using a combination of 454 pyrosequencing, protein mass spectrometry and quantitative real-time PCR, we identified 48 down-and 56 up-regulated chicken genes after Salmonella Enteritidis infection. The most inducible gene was that coding for MMP7, exhibiting a 5952 fold induction 9 days post-infection. An induction of greater than 100 fold was observed for IgG, IRG1, SAA, ExFABP, IL-22, TRAP6, MRP126, IFN gamma, iNOS, ES1, IL-1 beta, LYG2, IFIT5, IL-17, AVD, AH221 and SERPIN B. Since prostaglandin D2 synthase was upregulated and degrading hydroxyprostaglandin dehydrogenase was downregulated after the infection, prostaglandin must accumulate in the cecum of chickens infected with Salmonella Enteritidis. Finally, above mentioned signaling was dependent on the presence of a SPI1-encoded type III secretion system in Salmonella Enteritidis. The inflammation lasted for 2 weeks after which time the expression of the "inflammatory" genes returned back to basal levels and, instead, the expression of IgA and IgG increased. This points to an important role for immunoglobulins in the restoration of homeostasis in the cecum after infection.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

ELSHEIMER MATULOVÁ, Marta; Karolína VARMUŽOVÁ; František SISAK; Hana HAVÍČKOVÁ; Vladimír BABÁK; Karel STEJSKAL; Zbyněk ZDRÁHAL a Ivan RYCHLIK. Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis. VETERINARY RESEARCH. LONDON: BIOMED CENTRAL LTD, 2013, roč. 44, May, s. "nestránkováno", 11 s. ISSN 0928-4249. Dostupné z: https://doi.org/10.1186/1297-9716-44-37.

@article{1118819,
   author = {Elsheimer Matulová, Marta and Varmužová, Karolína and Sisak, František and Havíčková, Hana and Babák, Vladimír and Stejskal, Karel and Zdráhal, Zbyněk and Rychlik, Ivan},
   article_location = {LONDON},
   article_number = {May},
   doi = {https://doi.org/10.1186/1297-9716-44-37},
   keywords = {HEMATOPOIETIC LINEAGE; HS1 PROTEIN; CD8(+) T; TYPHIMURIUM; IDENTIFICATION; EXPANSION; SUBSTRATE; CELLS; GUT},
   language = {eng},
   issn = {0928-4249},
   journal = {VETERINARY RESEARCH},
   title = {Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis},
   url = {http://www.veterinaryresearch.org/content/44/1/37},
   volume = {44},
   year = {2013}
}

TY  - JOUR
ID  - 1118819
AU  - Elsheimer Matulová, Marta - Varmužová, Karolína - Sisak, František - Havíčková, Hana - Babák, Vladimír - Stejskal, Karel - Zdráhal, Zbyněk - Rychlik, Ivan
PY  - 2013
TI  - Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis
JF  - VETERINARY RESEARCH
VL  - 44
IS  - May
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - BIOMED CENTRAL LTD
SN  - 09284249
KW  - HEMATOPOIETIC LINEAGE
KW  - HS1 PROTEIN
KW  - CD8(+) T
KW  - TYPHIMURIUM
KW  - IDENTIFICATION
KW  - EXPANSION
KW  - SUBSTRATE
KW  - CELLS
KW  - GUT
UR  - http://www.veterinaryresearch.org/content/44/1/37
L2  - http://www.veterinaryresearch.org/content/44/1/37
N2  - The characterization of the immune response of chickens to Salmonella infection is usually limited to the quantification of expression of genes coding for cytokines, chemokines or antimicrobial peptides. However, processes occurring in the cecum of infected chickens are likely to be much more diverse. In this study we have therefore characterized the transcriptome and proteome in the chicken cecum after infection with Salmonella Enteritidis. Using a combination of 454 pyrosequencing, protein mass spectrometry and quantitative real-time PCR, we identified 48 down-and 56 up-regulated chicken genes after Salmonella Enteritidis infection. The most inducible gene was that coding for MMP7, exhibiting a 5952 fold induction 9 days post-infection. An induction of greater than 100 fold was observed for IgG, IRG1, SAA, ExFABP, IL-22, TRAP6, MRP126, IFN gamma, iNOS, ES1, IL-1 beta, LYG2, IFIT5, IL-17, AVD, AH221 and SERPIN B. Since prostaglandin D2 synthase was upregulated and degrading hydroxyprostaglandin dehydrogenase was downregulated after the infection, prostaglandin must accumulate in the cecum of chickens infected with Salmonella Enteritidis. Finally, above mentioned signaling was dependent on the presence of a SPI1-encoded type III secretion system in Salmonella Enteritidis. The inflammation lasted for 2 weeks after which time the expression of the "inflammatory" genes returned back to basal levels and, instead, the expression of IgA and IgG increased. This points to an important role for immunoglobulins in the restoration of homeostasis in the cecum after infection.
ER  -

ELSHEIMER MATULOVÁ, Marta; Karolína VARMUŽOVÁ; František SISAK; Hana HAVÍČKOVÁ; Vladimír BABÁK; Karel STEJSKAL; Zbyněk ZDRÁHAL a Ivan RYCHLIK. Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis. \textit{VETERINARY RESEARCH}. LONDON: BIOMED CENTRAL LTD, 2013, roč.~44, May, s.~''nestránkováno'', 11 s. ISSN~0928-4249. Dostupné z: https://doi.org/10.1186/1297-9716-44-37.

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