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@article{1119876,
author = {Krejčová, Ludmila and Húska, Dalibor and Hynek, David and Kopel, Pavel and Adam, Vojtech and Hubálek, Jaromír and Trnková, Libuše and Kizek, René},
article_number = {1},
keywords = {Influenza; Nanoparticles; Quantum Dots; Multi Target Detection; Voltammetry; Magnetic Particles; Hybridization; Automated Separation; Electrochemistry},
language = {eng},
issn = {1452-3981},
journal = {International Journal of Electrochemical Science},
title = {Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses' Specific Nucleic Acids},
volume = {8},
year = {2013}
}
TY - JOUR
ID - 1119876
AU - Krejčová, Ludmila - Húska, Dalibor - Hynek, David - Kopel, Pavel - Adam, Vojtech - Hubálek, Jaromír - Trnková, Libuše - Kizek, René
PY - 2013
TI - Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses' Specific Nucleic Acids
JF - International Journal of Electrochemical Science
VL - 8
IS - 1
SP - 689-702
EP - 689-702
SN - 14523981
KW - Influenza
KW - Nanoparticles
KW - Quantum Dots
KW - Multi Target Detection
KW - Voltammetry
KW - Magnetic Particles
KW - Hybridization
KW - Automated Separation
KW - Electrochemistry
N2 - The aim of this study was to suggest isolation protocol based on paramagnetic microparticles and multicolour electrochemical detection of three various influenza oligonucleotides (H1N1 influenza subtype, H3N2 influenza subtype and H5N1 influenza subtype) modified with nanoparticles (NPs) called quantum dots (QDs), which are made from zinc, cadmium or lead. Particularly, we described hybridization assay based on paramagnetic microparticles (MPs) and coupled with detection of three different influenza derived oligonucleotides (ODNs) labelled with QDs. Hybridization efficiency between NPs conjugated anti-senses and target nucleic acid hybrids was optimized and evaluated by the electrochemical analysis. The highest response of ODN-SH-NPs was determined for the hybridization temperature of 25 degrees C. Further, we tested detection of the complexes via peak coming from the presence of ODNs and/or from the presence of metal ions in QDs. Under the optimized conditions, LODs (3 S/N) based on the determination of metal ions in QDs label were estimated as follows: 0.1 ng/ml of ODN-SH-CdNPs, 0.5 ng/ml of ODN-SH-ZnNPs and 1 ng/ml of ODN-SH-PbNPs. Besides this we also correlated the results from ODNs and metal ions signals.
ER -
KREJČOVÁ, Ludmila, Dalibor HÚSKA, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ a René KIZEK. Using of Paramagnetic Microparticles and Quantum Dots for Isolation and Electrochemical Detection of Influenza Viruses' Specific Nucleic Acids. \textit{International Journal of Electrochemical Science}. 2013, roč.~8, č.~1, s.~689-702. ISSN~1452-3981.
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