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@article{1120400,
author = {Procházková, Jiřina and Kabátková, Markéta and Šmerdová, Lenka and Pacherník, Jiří and Sýkorová, Dominika and Kohoutek, Jiří and Simeckova, Pavlina and Hrubá, Eva and Kozubík, Alois and Machala, Miroslav and Vondráček, Jan},
article_location = {OXFORD},
article_number = {2},
doi = {http://dx.doi.org/10.1093/toxsci/kft110},
keywords = {aryl hydrocarbon receptor; dioxin; cell proliferation; plakoglobin; desmosomes; liver progenitor cells; cardiomyocytes},
language = {eng},
issn = {1096-6080},
journal = {Toxicological sciences},
note = {Opraven obor publikace},
title = {Aryl Hydrocarbon Receptor Negatively Regulates Expression of the Plakoglobin Gene (Jup)},
volume = {134},
year = {2013}
}
TY - JOUR
ID - 1120400
AU - Procházková, Jiřina - Kabátková, Markéta - Šmerdová, Lenka - Pacherník, Jiří - Sýkorová, Dominika - Kohoutek, Jiří - Simeckova, Pavlina - Hrubá, Eva - Kozubík, Alois - Machala, Miroslav - Vondráček, Jan
PY - 2013
TI - Aryl Hydrocarbon Receptor Negatively Regulates Expression of the Plakoglobin Gene (Jup)
JF - Toxicological sciences
VL - 134
IS - 2
SP - 258-270
EP - 258-270
PB - OXFORD UNIV PRESS
SN - 10966080
N1 - Opraven obor publikace
KW - aryl hydrocarbon receptor
KW - dioxin
KW - cell proliferation
KW - plakoglobin
KW - desmosomes
KW - liver progenitor cells
KW - cardiomyocytes
N2 - Plakoglobin is an important component of intercellular junctions, including both desmosomes and adherens junctions, which is known as a tumor suppressor. Although mutations in the plakoglobin gene (Jup) and/or changes in its protein levels have been observed in various disease states, including cancer progression or cardiovascular defects, the information about endogenous or exogenous stimuli orchestrating Jup expression is limited. Here we show that the aryl hydrocarbon receptor (AhR) may regulate Jup expression in a cell-specific manner. We observed a significant suppressive effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a model toxic exogenous activator of the AhR signaling, on Jup expression in a variety of experimental models derived from rodent tissues, including contact-inhibited rat liver progenitor cells (where TCDD induces cell proliferation), rat and mouse hepatoma cell models (where TCDD inhibits cell cycle progression), cardiac cells derived from the mouse embryonic stem cells, or cardiomyocytes isolated from neonatal rat hearts. The small interfering RNA (siRNA)mediated knockdown of AhR confirmed its role in both basal and TCDD-deregulated Jup expression. The analysis of genomic DNA located similar to 2.5kb upstream of rat Jup gene revealed a presence of evolutionarily conserved AhR binding motifs, which were confirmed upon their cloning into luciferase reporter construct. The siRNA-mediated knockdown of Jup expression affected both proliferation and attachment of liver progenitor cells. The present data indicate that the AhR may contribute to negative regulation of Jup gene expression in rodent cellular models, which may affect cell adherence and proliferation.
ER -
PROCHÁZKOVÁ, Jiřina, Markéta KABÁTKOVÁ, Lenka ŠMERDOVÁ, Jiří PACHERNÍK, Dominika SÝKOROVÁ, Jiří KOHOUTEK, Pavlina SIMECKOVA, Eva HRUBÁ, Alois KOZUBÍK, Miroslav MACHALA a Jan VONDRÁČEK. Aryl Hydrocarbon Receptor Negatively Regulates Expression of the Plakoglobin Gene (Jup). \textit{Toxicological sciences}. OXFORD: OXFORD UNIV PRESS, 2013, roč.~134, č.~2, s.~258-270. ISSN~1096-6080. Dostupné z: https://dx.doi.org/10.1093/toxsci/kft110.
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