Genome-wide transcriptional analysis of Drosophila larvae
infected by entomopathogenic nematodes shows involvement of
complement, recognition and extracellular matrix proteins

J 2014

Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins

AREFIN, Badrul; Lucie KUČEROVÁ; Pavel DOBEŠ; Robert MARKUS; Hynek STRNAD et al.

Základní údaje

Originální název

Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins

Autoři

AREFIN, Badrul; Lucie KUČEROVÁ; Pavel DOBEŠ; Robert MARKUS; Hynek STRNAD; Zhi WANG; Pavel HYRŠL; Michal ŽUROVEC a Ulrich THEOPOLD

Vydání

Journal of Innate Immunity, Basel, Karger Publishers, 2014, 1662-811X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.352

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/14:00074739

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Basement membrane; Coagulation; Hemocyte; Insect immunity; Nematodes; Recognition molecule; Thioester-containing proteins

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 10. 2019 10:06, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

AREFIN, Badrul; Lucie KUČEROVÁ; Pavel DOBEŠ; Robert MARKUS; Hynek STRNAD; Zhi WANG; Pavel HYRŠL; Michal ŽUROVEC a Ulrich THEOPOLD. Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. Journal of Innate Immunity. Basel: Karger Publishers, 2014, roč. 6, č. 2, s. 192-204. ISSN 1662-811X. Dostupné z: https://doi.org/10.1159/000353734.

@article{1121079,
   author = {Arefin, Badrul and Kučerová, Lucie and Dobeš, Pavel and Markus, Robert and Strnad, Hynek and Wang, Zhi and Hyršl, Pavel and Žurovec, Michal and Theopold, Ulrich},
   article_location = {Basel},
   article_number = {2},
   doi = {https://doi.org/10.1159/000353734},
   keywords = {Basement membrane; Coagulation; Hemocyte; Insect immunity; Nematodes; Recognition molecule; Thioester-containing proteins},
   language = {eng},
   issn = {1662-811X},
   journal = {Journal of Innate Immunity},
   title = {Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins},
   url = {http://www.karger.com/Article/FullText/353734},
   volume = {6},
   year = {2014}
}

TY  - JOUR
ID  - 1121079
AU  - Arefin, Badrul - Kučerová, Lucie - Dobeš, Pavel - Markus, Robert - Strnad, Hynek - Wang, Zhi - Hyršl, Pavel - Žurovec, Michal - Theopold, Ulrich
PY  - 2014
TI  - Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins
JF  - Journal of Innate Immunity
VL  - 6
IS  - 2
SP  - 192-204
EP  - 192-204
PB  - Karger Publishers
SN  - 1662811X
KW  - Basement membrane
KW  - Coagulation
KW  - Hemocyte
KW  - Insect immunity
KW  - Nematodes
KW  - Recognition molecule
KW  - Thioester-containing proteins
UR  - http://www.karger.com/Article/FullText/353734
L2  - http://www.karger.com/Article/FullText/353734
N2  - Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.
ER  -

AREFIN, Badrul; Lucie KUČEROVÁ; Pavel DOBEŠ; Robert MARKUS; Hynek STRNAD; Zhi WANG; Pavel HYRŠL; Michal ŽUROVEC a Ulrich THEOPOLD. Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. \textit{Journal of Innate Immunity}. Basel: Karger Publishers, 2014, roč.~6, č.~2, s.~192-204. ISSN~1662-811X. Dostupné z: https://doi.org/10.1159/000353734.

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