Antibacterial activity and cell viability of hyaluronan fiber
with silver

J 2013

Antibacterial activity and cell viability of hyaluronan fiber with silver

ABDEL-MOHSEN, A.M., Radim HRDINA, Ladislav BURGERT, Rasha M. ABDEL-RAHMAN, Martina MORAVCOVÁ et. al.

Základní údaje

Originální název

Antibacterial activity and cell viability of hyaluronan fiber with silver

Autoři

ABDEL-MOHSEN, A.M. (818 Egypt), Radim HRDINA (203 Česká republika), Ladislav BURGERT (203 Česká republika), Rasha M. ABDEL-RAHMAN (818 Egypt), Martina MORAVCOVÁ (203 Česká republika, domácí), Daniela ŠMEJKALOVÁ (203 Česká republika), Michal KOLÁŘ (203 Česká republika), M. PEKAŘ (203 Česká republika) a A.S. ALY (818 Egypt)

Vydání

Carbohydrate Polymers, 2013, 0144-8617

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10401 Organic chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.916

Kód RIV

RIV/00216224:14310/13:00081912

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.carbpol.2012.08.098

UT WoS

000315616900030

Klíčová slova anglicky

Green chemistry; Hyaluronan fiber; Silver nanoparticles; Cell viability; Antibacterial activity; Medical applications

Štítky

AKR, rivok

Změněno: 26. 4. 2016 15:08, Ing. Andrea Mikešková

Anotace

V originále

Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.

Citovat

ABDEL-MOHSEN, A.M., Radim HRDINA, Ladislav BURGERT, Rasha M. ABDEL-RAHMAN, Martina MORAVCOVÁ, Daniela ŠMEJKALOVÁ, Michal KOLÁŘ, M. PEKAŘ a A.S. ALY. Antibacterial activity and cell viability of hyaluronan fiber with silver. Carbohydrate Polymers. 2013, roč. 92, č. 2, s. 1177-1187. ISSN 0144-8617. Dostupné z: https://dx.doi.org/10.1016/j.carbpol.2012.08.098.

@article{1122541,
   author = {AbdelandMohsen, A.M. and Hrdina, Radim and Burgert, Ladislav and AbdelandRahman, Rasha M. and Moravcová, Martina and Šmejkalová, Daniela and Kolář, Michal and Pekař, M. and Aly, A.S.},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.carbpol.2012.08.098},
   keywords = {Green chemistry; Hyaluronan fiber; Silver nanoparticles; Cell viability; Antibacterial activity; Medical applications},
   language = {eng},
   issn = {0144-8617},
   journal = {Carbohydrate Polymers},
   title = {Antibacterial activity and cell viability of hyaluronan fiber with silver},
   volume = {92},
   year = {2013}
}

TY  - JOUR
ID  - 1122541
AU  - Abdel-Mohsen, A.M. - Hrdina, Radim - Burgert, Ladislav - Abdel-Rahman, Rasha M. - Moravcová, Martina - Šmejkalová, Daniela - Kolář, Michal - Pekař, M. - Aly, A.S.
PY  - 2013
TI  - Antibacterial activity and cell viability of hyaluronan fiber with silver
JF  - Carbohydrate Polymers
VL  - 92
IS  - 2
SP  - 1177-1187
EP  - 1177-1187
SN  - 01448617
KW  - Green chemistry
KW  - Hyaluronan fiber
KW  - Silver nanoparticles
KW  - Cell viability
KW  - Antibacterial activity
KW  - Medical applications
N2  - Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.
ER  -

ABDEL-MOHSEN, A.M., Radim HRDINA, Ladislav BURGERT, Rasha M. ABDEL-RAHMAN, Martina MORAVCOVÁ, Daniela ŠMEJKALOVÁ, Michal KOLÁŘ, M. PEKAŘ a A.S. ALY. Antibacterial activity and cell viability of hyaluronan fiber with silver. \textit{Carbohydrate Polymers}. 2013, roč.~92, č.~2, s.~1177-1187. ISSN~0144-8617. Dostupné z: https://dx.doi.org/10.1016/j.carbpol.2012.08.098.

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