Simultaneous quantification of energetically important
metabolites in various cell types by capillary zone
electrophoresis

J 2013

Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis

MUSILOVÁ, Jindra; Bořivoj KLEJDUS a Zdeněk GLATZ

Základní údaje

Originální název

Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis

Název česky

Simultání stanopvení energeticky důležitých metabolitů v různých typech buněk pomocí CZE

Autoři

MUSILOVÁ, Jindra; Bořivoj KLEJDUS a Zdeněk GLATZ

Vydání

Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2013, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.594

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00066386

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1002/jssc.201300926

UT WoS

000327556300021

Klíčová slova česky

metabolomika; nukleotidy; Paracoccus denitrificans; kmenové buňky; systémová biologie

Klíčová slova anglicky

metabolomics; nucleotides; Paracoccus denitrificans; stem cells; system biology

Štítky

AKR, rivok

Změněno: 29. 4. 2014 09:39, Ing. Zdeňka Rašková

Anotace

ORIG CZ

V originále

A new capillary zone electrophoretic method was developed for the determination of twelve purine and pyrimidine nucleotides, two adenine coenzymes and their reduced forms, and acetyl coenzyme A in various cell extracts. As the concentration levels of these metabolites in living cells are low; the capillary zone electrophoresis was combined with field-enhanced sample stacking. As a result, the separation conditions were optimised to achieve a suitable resolution at the relatively high sample volume provided by this on-line pre-concentration technique.

Česky

Byla vyprcována nová metoda pro stanovení 12 nukleotidů, NAD, NADP včetně jejich redukovaných forem a AcetylCoA pomocí CZE v kombinaci se stackingem.

Návaznosti

EE2.3.20.0182, projekt VaV

Název: Vytvoření multidisciplinárního výzkumného a vzdělávacího centra bioanalytických technologií

GAP206/11/0009, projekt VaV

Název: Kapilární electroforéza jako nástroj pro metabolomické studie (Akronym: CE metabolomics)

Investor: Grantová agentura ČR, Capillary electrophoresis as a member of the metabolomic analytical toolbox

Citovat

MUSILOVÁ, Jindra; Bořivoj KLEJDUS a Zdeněk GLATZ. Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2013, roč. 36, č. 23, s. 3807-3812. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201300926.

@article{1124193,
   author = {Musilová, Jindra and Klejdus, Bořivoj and Glatz, Zdeněk},
   article_location = {Weinheim},
   article_number = {23},
   doi = {https://doi.org/10.1002/jssc.201300926},
   keywords = {metabolomics; nucleotides; Paracoccus denitrificans; stem cells; system biology},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300926/abstract},
   volume = {36},
   year = {2013}
}

TY  - JOUR
ID  - 1124193
AU  - Musilová, Jindra - Klejdus, Bořivoj - Glatz, Zdeněk
PY  - 2013
TI  - Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis
JF  - Journal of Separation Science
VL  - 36
IS  - 23
SP  - 3807-3812
EP  - 3807-3812
PB  - WILEY-VCH Verlag GmbH & Co.
SN  - 16159306
KW  - metabolomics
KW  - nucleotides
KW  - Paracoccus denitrificans
KW  - stem cells
KW  - system biology
UR  - http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300926/abstract
N2  - A new capillary zone electrophoretic method was developed for the determination of twelve purine and pyrimidine nucleotides, two adenine coenzymes and their reduced forms, and acetyl coenzyme A in various cell extracts. As the concentration levels of these metabolites in living cells are low; the capillary zone electrophoresis was combined with field-enhanced sample stacking. As a result, the separation conditions were optimised to achieve a suitable resolution at the relatively high sample volume provided by this on-line pre-concentration technique.
ER  -

MUSILOVÁ, Jindra; Bořivoj KLEJDUS a Zdeněk GLATZ. Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis. \textit{Journal of Separation Science}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co., 2013, roč.~36, č.~23, s.~3807-3812. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201300926.

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