Single-Base Resolution Sequence-Directed Nucleosome Mapping

J 2013

Single-Base Resolution Sequence-Directed Nucleosome Mapping

TRIFONOV, Eduard Nikolajevič a Jan HAPALA

Základní údaje

Originální název

Single-Base Resolution Sequence-Directed Nucleosome Mapping

Autoři

TRIFONOV, Eduard Nikolajevič (376 Izrael, garant, domácí) a Jan HAPALA (203 Česká republika, domácí)

Vydání

Israel Journal of Chemistry, WEINHEIM, WILEY-V C H VERLAG GMBH, 2013, 0021-2148

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.561

Kód RIV

RIV/00216224:14740/13:00069571

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1002/ijch.201200074

UT WoS

000317859800003

Klíčová slova anglicky

DNA; ucleobases; nucleosome positioning; regulation of transcription; TATA box

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 10. 2013 11:09, Olga Křížová

Anotace

V originále

Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data. Two computational approaches to the accurate positioning of nucleosomes, based on energy calculations and on sequence patterndirected mapping, are currently in different stages of progress.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

TRIFONOV, Eduard Nikolajevič a Jan HAPALA. Single-Base Resolution Sequence-Directed Nucleosome Mapping. Israel Journal of Chemistry. WEINHEIM: WILEY-V C H VERLAG GMBH, 2013, roč. 53, 3-4, s. 144-146. ISSN 0021-2148. Dostupné z: https://dx.doi.org/10.1002/ijch.201200074.

@article{1124392,
   author = {Trifonov, Eduard Nikolajevič and Hapala, Jan},
   article_location = {WEINHEIM},
   article_number = {3-4},
   doi = {http://dx.doi.org/10.1002/ijch.201200074},
   keywords = {DNA; ucleobases; nucleosome positioning; regulation of transcription; TATA box},
   language = {eng},
   issn = {0021-2148},
   journal = {Israel Journal of Chemistry},
   title = {Single-Base Resolution Sequence-Directed Nucleosome Mapping},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/ijch.201200074/abstract},
   volume = {53},
   year = {2013}
}

TY  - JOUR
ID  - 1124392
AU  - Trifonov, Eduard Nikolajevič - Hapala, Jan
PY  - 2013
TI  - Single-Base Resolution Sequence-Directed Nucleosome Mapping
JF  - Israel Journal of Chemistry
VL  - 53
IS  - 3-4
SP  - 144-146
EP  - 144-146
PB  - WILEY-V C H VERLAG GMBH
SN  - 00212148
KW  - DNA
KW  - ucleobases
KW  - nucleosome positioning
KW  - regulation of transcription
KW  - TATA box
UR  - http://onlinelibrary.wiley.com/doi/10.1002/ijch.201200074/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/ijch.201200074/abstract
N2  - Frequently used nucleosome mapping techniques, both experimental and computational ones, pursue a rather simple task to determine whether a given sequence segment is likely to belong to a nucleosome, thus measuring the nucleosome occupancy along the sequence. A more ambitious task is to determine the position with high resolution, so that not only the approximate translational position of the nucleosome on DNA would be known, but also the rotational setting of the DNA. The rotational setting is important to know since the binding of various transcription factors to the nucleosome DNA crucially depends on the accessibility of the respective recognition sequences. The only experimental technique that provides the highest possible accuracy of the positioning is crystallization of the nucleosomes reconstituted on specific sequences, with subsequent solving of their structures from x-ray diffraction data. Two computational approaches to the accurate positioning of nucleosomes, based on energy calculations and on sequence patterndirected mapping, are currently in different stages of progress.
ER  -

TRIFONOV, Eduard Nikolajevič a Jan HAPALA. Single-Base Resolution Sequence-Directed Nucleosome Mapping. \textit{Israel Journal of Chemistry}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2013, roč.~53, 3-4, s.~144-146. ISSN~0021-2148. Dostupné z: https://dx.doi.org/10.1002/ijch.201200074.

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