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@article{1124595, author = {Šebesta, Marek and Burkovics, Peter and Juhasz, Szilvia and Zhang, Sufang and Szabo, Judit and Lee, Marietta Y. W. T. and Haracska, Lajos and Krejčí, Lumír}, article_location = {AMSTERDAM}, article_number = {9}, doi = {http://dx.doi.org/10.1016/j.dnarep.2013.05.001}, keywords = {TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution}, language = {eng}, issn = {1568-7864}, journal = {DNA Repair}, title = {Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans}, volume = {12}, year = {2013} }
TY - JOUR ID - 1124595 AU - Šebesta, Marek - Burkovics, Peter - Juhasz, Szilvia - Zhang, Sufang - Szabo, Judit - Lee, Marietta Y. W. T. - Haracska, Lajos - Krejčí, Lumír PY - 2013 TI - Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans JF - DNA Repair VL - 12 IS - 9 SP - 691-698 EP - 691-698 PB - ELSEVIER SCIENCE BV SN - 15687864 KW - TLS polymerases KW - Homologous recombination KW - DNA repair synthesis KW - D-loop KW - Reconstitution N2 - Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes. ER -
ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO, Marietta Y. W. T. LEE, Lajos HARACSKA and Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. \textit{DNA Repair}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, vol.~12, No~9, p.~691-698. ISSN~1568-7864. Available from: https://dx.doi.org/10.1016/j.dnarep.2013.05.001.
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