Role of PCNA and TLS polymerases in D-loop extension during
homologous  recombination in humans

J 2013

Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO et. al.

Basic information

Original name

Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

Authors

ŠEBESTA, Marek (703 Slovakia, belonging to the institution), Peter BURKOVICS (348 Hungary, belonging to the institution), Szilvia JUHASZ (348 Hungary), Sufang ZHANG (840 United States of America), Judit SZABO (348 Hungary, belonging to the institution), Marietta Y. W. T. LEE (840 United States of America), Lajos HARACSKA (348 Hungary) and Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)

Edition

DNA Repair, AMSTERDAM, ELSEVIER SCIENCE BV, 2013, 1568-7864

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.362

RIV identification code

RIV/00216224:14110/13:00066407

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.dnarep.2013.05.001

UT WoS

000323468400001

Keywords in English

TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution

Abstract

V originále

Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.

Links

EE2.3.20.0011, research and development project

Name: Centrum výzkumu pluripotentních buněk a nestability genomu

GAP207/12/2323, research and development project

Name: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Czech Science Foundation

GA13-26629S, research and development project

Name: SUMO a stability genomu

Investor: Czech Science Foundation

Citovat

ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO, Marietta Y. W. T. LEE, Lajos HARACSKA and Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. DNA Repair. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, vol. 12, No 9, p. 691-698. ISSN 1568-7864. Available from: https://dx.doi.org/10.1016/j.dnarep.2013.05.001.

@article{1124595,
   author = {Šebesta, Marek and Burkovics, Peter and Juhasz, Szilvia and Zhang, Sufang and Szabo, Judit and Lee, Marietta Y. W. T. and Haracska, Lajos and Krejčí, Lumír},
   article_location = {AMSTERDAM},
   article_number = {9},
   doi = {http://dx.doi.org/10.1016/j.dnarep.2013.05.001},
   keywords = {TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution},
   language = {eng},
   issn = {1568-7864},
   journal = {DNA Repair},
   title = {Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans},
   volume = {12},
   year = {2013}
}

TY  - JOUR
ID  - 1124595
AU  - Šebesta, Marek - Burkovics, Peter - Juhasz, Szilvia - Zhang, Sufang - Szabo, Judit - Lee, Marietta Y. W. T. - Haracska, Lajos - Krejčí, Lumír
PY  - 2013
TI  - Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
JF  - DNA Repair
VL  - 12
IS  - 9
SP  - 691-698
EP  - 691-698
PB  - ELSEVIER SCIENCE BV
SN  - 15687864
KW  - TLS polymerases
KW  - Homologous recombination
KW  - DNA repair synthesis
KW  - D-loop
KW  - Reconstitution
N2  - Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
ER  -

ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO, Marietta Y. W. T. LEE, Lajos HARACSKA and Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. \textit{DNA Repair}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, vol.~12, No~9, p.~691-698. ISSN~1568-7864. Available from: https://dx.doi.org/10.1016/j.dnarep.2013.05.001.

Detailed Information on Publication Record