Role of PCNA and TLS polymerases in D-loop extension during
homologous  recombination in humans

J 2013

Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO et. al.

Základní údaje

Originální název

Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans

Autoři

ŠEBESTA, Marek (703 Slovensko, domácí), Peter BURKOVICS (348 Maďarsko, domácí), Szilvia JUHASZ (348 Maďarsko), Sufang ZHANG (840 Spojené státy), Judit SZABO (348 Maďarsko, domácí), Marietta Y. W. T. LEE (840 Spojené státy), Lajos HARACSKA (348 Maďarsko) a Lumír KREJČÍ (203 Česká republika, garant, domácí)

Vydání

DNA Repair, AMSTERDAM, ELSEVIER SCIENCE BV, 2013, 1568-7864

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.362

Kód RIV

RIV/00216224:14110/13:00066407

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.dnarep.2013.05.001

UT WoS

000323468400001

Klíčová slova anglicky

TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution

Štítky

podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 1. 2014 12:17, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.

Návaznosti

EE2.3.20.0011, projekt VaV

Název: Centrum výzkumu pluripotentních buněk a nestability genomu

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

Citovat

ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO, Marietta Y. W. T. LEE, Lajos HARACSKA a Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. DNA Repair. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč. 12, č. 9, s. 691-698. ISSN 1568-7864. Dostupné z: https://dx.doi.org/10.1016/j.dnarep.2013.05.001.

@article{1124595,
   author = {Šebesta, Marek and Burkovics, Peter and Juhasz, Szilvia and Zhang, Sufang and Szabo, Judit and Lee, Marietta Y. W. T. and Haracska, Lajos and Krejčí, Lumír},
   article_location = {AMSTERDAM},
   article_number = {9},
   doi = {http://dx.doi.org/10.1016/j.dnarep.2013.05.001},
   keywords = {TLS polymerases; Homologous recombination; DNA repair synthesis; D-loop; Reconstitution},
   language = {eng},
   issn = {1568-7864},
   journal = {DNA Repair},
   title = {Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans},
   volume = {12},
   year = {2013}
}

TY  - JOUR
ID  - 1124595
AU  - Šebesta, Marek - Burkovics, Peter - Juhasz, Szilvia - Zhang, Sufang - Szabo, Judit - Lee, Marietta Y. W. T. - Haracska, Lajos - Krejčí, Lumír
PY  - 2013
TI  - Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans
JF  - DNA Repair
VL  - 12
IS  - 9
SP  - 691-698
EP  - 691-698
PB  - ELSEVIER SCIENCE BV
SN  - 15687864
KW  - TLS polymerases
KW  - Homologous recombination
KW  - DNA repair synthesis
KW  - D-loop
KW  - Reconstitution
N2  - Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol delta, TLS polymerases, including Pol eta and Pol kappa, also can extend D-loops. In vivo characterization reveals that Pol eta and Pol kappa are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
ER  -

ŠEBESTA, Marek, Peter BURKOVICS, Szilvia JUHASZ, Sufang ZHANG, Judit SZABO, Marietta Y. W. T. LEE, Lajos HARACSKA a Lumír KREJČÍ. Role of PCNA and TLS polymerases in D-loop extension during homologous recombination in humans. \textit{DNA Repair}. AMSTERDAM: ELSEVIER SCIENCE BV, 2013, roč.~12, č.~9, s.~691-698. ISSN~1568-7864. Dostupné z: https://dx.doi.org/10.1016/j.dnarep.2013.05.001.

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