A solution study of chemical equilibria of physiologically
active nucleobases

D 2013

A solution study of chemical equilibria of physiologically active nucleobases

NETROUFALOVÁ, Petra; Přemysl LUBAL; Pavel BUČEK a Libuše TRNKOVÁ

Základní údaje

Originální název

A solution study of chemical equilibria of physiologically active nucleobases

Autoři

NETROUFALOVÁ, Petra; Přemysl LUBAL; Pavel BUČEK a Libuše TRNKOVÁ

Vydání

Brno, Structure and dynamics of biological macromolecules A biophysical workshop, s. 25-25, 2013

Nakladatel

Masarykova univerzita

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-6341-9

Klíčová slova anglicky

DNA;RNA;nucleobase;molecular absorption spectroscopy;UV-VIS;OPIUM;

Štítky

Změněno: 17. 5. 2018 15:25, Ing. Nicole Zrilić

Anotace

V originále

Nucleobases are building blocks of nucleic acids (DNA, RNA) where they are responsible for the transfer of genetic information. The structure and properties of nucleic acids can be influenced by their protonation and/or their complexation with metal ions [1]. Some structurally modified nucleobases and nucleosides exhibit physiological activity and therefore they are used as drugs [2]. This contribution is focused on the study of protonation constants of adenine-like compounds. They were determined by means of molecular absorption spectroscopy in UV region (I = 0.15 mol.l-1 M NaCl, t = 25 degrees C) and the experimental data were evaluated by OPIUM program. In addition, the temperature and ionic-strength dependence of protonation constants of studied ligands was used to estimate thermodynamic parameters (deltaH0, deltaS0, protonation constant(s) for infinite dilution). In some cases, the luminescence emission spectroscopy was also employed due to significantly high quantum yield of some compounds. Some examples of ligand complexation by zinc metal ion will be presented and their importance for bioligand speciation in vivo will be discussed.

Návaznosti

LH13053, projekt VaV

Název: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí (Akronym: CECAP)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Chemická a elektrochemická analýza purinových derivátů

ME09065, projekt VaV

Název: Výzkum nových detekčních systémů na bázi senzorových polí pro použití ve speciační analýze

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkum nových detekčních systémů na bázi senzorových polí pro použití ve speciační analýze, Program výzkumu a vývoje KONTAKT (ME)

Citovat

NETROUFALOVÁ, Petra; Přemysl LUBAL; Pavel BUČEK a Libuše TRNKOVÁ. A solution study of chemical equilibria of physiologically active nucleobases. In Naděžda Vaškovicová;Jiří Kozelka. Structure and dynamics of biological macromolecules A biophysical workshop. Brno: Masarykova univerzita, 2013, s. 25. ISBN 978-80-210-6341-9.

@inproceedings{1125480,
   author = {Netroufalová, Petra and Lubal, Přemysl and Buček, Pavel and Trnková, Libuše},
   address = {Brno},
   booktitle = {Structure and dynamics of biological macromolecules A biophysical workshop},
   editor = {Naděžda Vaškovicová;Jiří Kozelka},
   keywords = {DNA;RNA;nucleobase;molecular absorption spectroscopy;UV-VIS;OPIUM;},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-6341-9},
   note = {Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+},
   pages = {25-25},
   publisher = {Masarykova univerzita},
   title = {A solution study of chemical equilibria of physiologically active nucleobases},
   year = {2013}
}

TY  - CONF
ID  - 1125480
AU  - Netroufalová, Petra - Lubal, Přemysl - Buček, Pavel - Trnková, Libuše
PY  - 2013
TI  - A solution study of chemical equilibria of physiologically active nucleobases
PB  - Masarykova univerzita
CY  - Brno
SN  - 9788021063419
N1  - Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+
KW  - DNA;RNA;nucleobase;molecular absorption spectroscopy;UV-VIS;OPIUM;
N2  - Nucleobases are building blocks of nucleic acids (DNA, RNA) where they are responsible for the transfer of genetic information. The structure and properties of nucleic acids can be influenced by their protonation and/or their complexation with metal ions [1]. Some structurally modified nucleobases and nucleosides exhibit physiological activity and therefore they are used as drugs [2]. This contribution is focused on the study of protonation constants of adenine-like compounds. They were determined by means of molecular absorption spectroscopy in UV region (I = 0.15 mol.l-1 M NaCl, t = 25 degrees C) and the experimental data were evaluated by OPIUM program. In addition, the temperature and ionic-strength dependence of protonation constants of studied ligands was used to estimate thermodynamic parameters (deltaH0, deltaS0, protonation constant(s) for infinite dilution). In some cases, the luminescence emission spectroscopy was also employed due to significantly high quantum yield of some compounds. Some examples of ligand complexation by zinc metal ion will be presented and their importance for bioligand speciation in vivo will be discussed.
ER  -

NETROUFALOVÁ, Petra; Přemysl LUBAL; Pavel BUČEK a Libuše TRNKOVÁ. A solution study of chemical equilibria of physiologically active nucleobases. In Naděžda Vaškovicová;Jiří Kozelka. \textit{Structure and dynamics of biological macromolecules A biophysical workshop}. Brno: Masarykova univerzita, 2013, s.~25. ISBN~978-80-210-6341-9.

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