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@article{1126194,
author = {Wiewiorka, Ondřej and Dastych, Milan and Čermáková, Zdeňka},
article_location = {LONDON},
article_number = {5},
doi = {http://dx.doi.org/10.3109/00365513.2013.794300},
keywords = {Creatinine; Dicynone; ethamsylate; enzymatic assay; interference},
language = {eng},
issn = {0036-5513},
journal = {SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION},
title = {Strong negative interference of ethamsylate (Dicynone (R)) in serum creatinine quantification via enzymatic assay using Trinder reaction},
volume = {73},
year = {2013}
}
TY - JOUR
ID - 1126194
AU - Wiewiorka, Ondřej - Dastych, Milan - Čermáková, Zdeňka
PY - 2013
TI - Strong negative interference of ethamsylate (Dicynone (R)) in serum creatinine quantification via enzymatic assay using Trinder reaction
JF - SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
VL - 73
IS - 5
SP - 449-451
EP - 449-451
PB - INFORMA HEALTHCARE
SN - 00365513
KW - Creatinine
KW - Dicynone
KW - ethamsylate
KW - enzymatic assay
KW - interference
N2 - Background. With discrepancies encountered as early as the verification of enzymatic method for quantification of serum creatinine, our research pointed to a later confirmed interference caused by a compound called ethamsylate present in the commonly used antihemorrhagic drug Dicynone. Methods. We measured concentrations of creatinine of 10 patients with blood taken before and 15 minutes after the intravenous administration of a 500 mg dose of Dicynone. The creatinine concentration was determined using Jaffe method and enzymatic method that utilize Trinder reaction (Roche) in analyzer Cobas c 501 (Roche AG, Basel, Switzerland). We also monitored concentration of blood creatinine in three patients before and 15 minutes after application of Dicynone (500 mg i.v.) and in the following 6th, 12th, 18th, and 24th hours. Results. We discovered a significant negative bias in creatinine results using enzymatic assay with Trinder reaction in blood taken 15 min after i.v. application of 500 mg Dicynone to patients compared to their pre-application values (average decrease of 47%). Unlike this, the results of compensated Jaffe method yielded steady results in all samples (average deviation 0.6% from original values). However, 12 h after the drug administration comparable results were seen as before the administration. Conclusion. Considering the strong negative interference of ethamsylate in enzymatic assay using Trinder reaction for creatinine quantification, blood from patients with prescribed Dicynone should be taken at least 12 h after the last application of the drug for obtaining the correct creatinine values.
ER -
WIEWIORKA, Ondřej, Milan DASTYCH a Zdeňka ČERMÁKOVÁ. Strong negative interference of ethamsylate (Dicynone (R)) in serum creatinine quantification via enzymatic assay using Trinder reaction. \textit{SCANDINAVIAN JOURNAL OF CLINICAL \&{} LABORATORY INVESTIGATION}. LONDON: INFORMA HEALTHCARE, 2013, roč.~73, č.~5, s.~449-451. ISSN~0036-5513. Dostupné z: https://dx.doi.org/10.3109/00365513.2013.794300.
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