Complementary matrix-assisted laser desorption/ionization and
inductively coupled plasma mass spectrometry detection of
single separation record

a 2013

Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record

PREISLER, Jan; Iva TOMALOVÁ; Pavla FOLTYNOVÁ; Ondřej POLANSKÝ; Viktor KANICKÝ et. al.

Základní údaje

Originální název

Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record

Autoři

PREISLER, Jan (203 Česká republika, garant, domácí); Iva TOMALOVÁ (203 Česká republika, domácí); Pavla FOLTYNOVÁ (203 Česká republika, domácí); Ondřej POLANSKÝ (203 Česká republika, domácí) a Viktor KANICKÝ (203 Česká republika, domácí)

Vydání

20th International Symposium on Electro- and Liquid Phase-Separation Techniques, 2013. 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14740/13:00066470

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

mass spectrometry; MALDI MS; SALD ICP MS; metalloprotein; single separation run

Změněno: 28. 10. 2013 10:17, Mgr. Iva Benešová, Ph.D.

Anotace

V originále

The analyses of native metal-protein and metal-metabolite complexes by hyphenated techniques that combine separation with a specific detection is of growing importance. Using a liquid junction, capillary electrophoresis (CE) was coupled in the off-line mode to both matrix-assisted laser desorption/ionization mass spectrometry (MALDI) and substrate-assisted laser desorption inductively-coupled plasma (SALD ICP) mass spectrometry (MS). Here we present a new approach for an off-line multidetection platform for metalloprotein/metallopeptide analysis of fractions collected from a single CE run. CE fractions are collected on a special target and analyzed consecutively with MALDI MS and SALD ICP MS. The concept is demonstrated on the CE-MALDI/SALD ICP MS analysis of rabbit liver metallothionein (MT) isoforms: The mixture of MT isoforms was separated in uncoated fused silica capillary (70 cm, 75um) using 20 mM ammonium bicarbonate buffer, pH 7.4 as background electrolyte. Fractions from CE were collected on polyethyleneglycol target with a thin golden layer in a subatmospheric deposition chamber. After matrix addition, MALDI mass spectra of MT apoforms were recorded from the fractions. Finally, SALD ICP provided information on Cd content in the fractions. Alternatively, MALDI mass spectra of un dissociated MT complexes from selected fractions may be recorded using a colder MALDI matrix. In summary, it was shown that a single CE separation yielded both proteomic and metallomic information. The off-line approach is a viable alternative to on-line coupling with electrospray/nebulizer ICP MS and it does not require both mass spectrometers physically close to CE. In addition, the fractions may be archived and subjected to laser-induced fluorescence detection or on target digestion.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GAP206/12/0538, projekt VaV

Název: Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii

Investor: Grantová agentura ČR, Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii

Citovat

PREISLER, Jan; Iva TOMALOVÁ; Pavla FOLTYNOVÁ; Ondřej POLANSKÝ a Viktor KANICKÝ. Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record. In 20th International Symposium on Electro- and Liquid Phase-Separation Techniques, 2013. 2013.

@proceedings{1127504,
   author = {Preisler, Jan and Tomalová, Iva and Foltynová, Pavla and Polanský, Ondřej and Kanický, Viktor},
   booktitle = {20th International Symposium on Electro- and Liquid Phase-Separation Techniques, 2013.},
   keywords = {mass spectrometry; MALDI MS; SALD ICP MS; metalloprotein; single separation run},
   language = {eng},
   title = {Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record},
   year = {2013}
}

TY  - CONF
ID  - 1127504
AU  - Preisler, Jan - Tomalová, Iva - Foltynová, Pavla - Polanský, Ondřej - Kanický, Viktor
PY  - 2013
TI  - Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record
KW  - mass spectrometry
KW  - MALDI MS
KW  - SALD ICP MS
KW  - metalloprotein
KW  - single separation run
N2  - The analyses of native metal-protein and metal-metabolite complexes by hyphenated techniques that combine separation with a specific detection is of growing importance. Using a liquid junction, capillary electrophoresis (CE) was coupled in the off-line mode to both matrix-assisted laser desorption/ionization mass spectrometry (MALDI) and substrate-assisted laser desorption inductively-coupled plasma (SALD ICP) mass spectrometry (MS). Here we present a new approach for an off-line multidetection platform for metalloprotein/metallopeptide analysis of fractions collected from a single CE run. CE fractions are collected on a special target and analyzed consecutively with MALDI MS and SALD ICP MS. The concept is demonstrated on the CE-MALDI/SALD ICP MS analysis of rabbit liver metallothionein (MT) isoforms: The mixture of MT isoforms was separated in uncoated fused silica capillary (70 cm, 75um) using 20 mM ammonium bicarbonate buffer, pH 7.4 as background electrolyte. Fractions from CE were collected on polyethyleneglycol target with a thin golden layer in a subatmospheric deposition chamber. After matrix addition, MALDI mass spectra of MT apoforms were recorded from the fractions. Finally, SALD ICP provided information on Cd content in the fractions. Alternatively, MALDI mass spectra of un dissociated MT complexes from selected fractions may be recorded using a colder MALDI matrix. In summary, it was shown that a single CE separation yielded both proteomic and metallomic information. The off-line approach is a viable alternative to on-line coupling with electrospray/nebulizer ICP MS and it does not require both mass spectrometers physically close to CE. In addition, the fractions may be archived and subjected to laser-induced fluorescence detection or on target digestion.
ER  -

PREISLER, Jan; Iva TOMALOVÁ; Pavla FOLTYNOVÁ; Ondřej POLANSKÝ a Viktor KANICKÝ. Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record. In \textit{20th International Symposium on Electro- and Liquid Phase-Separation Techniques, 2013.}. 2013.

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