Genes of the microRNA biogenesis pathway are deregulated in
Colorectal cancer

a 2013

Genes of the microRNA biogenesis pathway are deregulated in Colorectal cancer

SLABÝ, Ondřej; Petra VYCHYTILOVÁ; Marek SVOBODA a Rostislav VYZULA

Základní údaje

Originální název

Genes of the microRNA biogenesis pathway are deregulated in Colorectal cancer

Autoři

SLABÝ, Ondřej; Petra VYCHYTILOVÁ ; Marek SVOBODA a Rostislav VYZULA

Vydání

3rd World Congress on Cancer Science and Therapy, CA, USA, 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

ISSN

Klíčová slova anglicky

microRNA; biogenesis; colorectal cancer; therapeutic targets; biomarkers

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 11. 2013 10:58, Mgr. Petra Vychytilová, Ph.D.

Anotace

V originále

MicroRNAs (miRNAs) are small non-coding RNAs 18-25 nt long that regulate gene expression on post-transcriptional level. Therefore, their production and maturation have to be strictly regulated. Their biogenesis starts with the transcription, which is followed by several steps that lead to processing of primary transcripts to mature miRNAs. Each step of this pathway is sophistically regulated and any disruption of control mechanisms may leads to the cancer occurrence. The aim of this study was to analyse the expression of the crucial genes involved in the biogenesis of miRNAs in colorectal cancer (CRC). Expression of 19 selected genes has been analysed in tumor tissues of 120 clinically characterized patients with CRC and in 120 parallel healthy tissues by qRT-PCR. Using Wilcoxon test, genes with different expression between tumor and healthy tissue have been identified. Subsequently, Kruskal-Wallis test has been used to find any correlation with the clinical-pathological features of the patients. We have found significantly higher expression of POLRIIA, ADAR, ADARB1, DGCR8, DDX5, DDX17, DDX20, DROSHA, XPO5, EIF2C1-C4, TARBP2, TNRC6A, GEMIN3, and DICER1 (P < 0.0001) and significantly lower expression of LIN28A in tumor tissue of CRC patients. Moreover, negative correlation between the expression of EIF2C3 and clinical stage of patients (P = 0.0017) and grade (P = 0.0151) and positive correlation between the expression of DICER1 (P = 0.0230) and DROSHA (P = 0.0212) and grade of patients has been observed. Our results show that changes in the expression of genes associated with biogenesis of miRNAs may be associated not only with the origin, but also with the progression of CRC and therefore, these molecules could serve as potential new biomarkers or therapeutic targets.

Návaznosti

NT13549, projekt VaV

Název: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem

Citovat

SLABÝ, Ondřej; Petra VYCHYTILOVÁ; Marek SVOBODA a Rostislav VYZULA. Genes of the microRNA biogenesis pathway are deregulated in Colorectal cancer. In 3rd World Congress on Cancer Science and Therapy, CA, USA. 2013. ISSN 1948-5956.

@proceedings{1128521,
   author = {Slabý, Ondřej and Vychytilová, Petra and Svoboda, Marek and Vyzula, Rostislav},
   booktitle = {3rd World Congress on Cancer Science and Therapy, CA, USA},
   keywords = {microRNA; biogenesis; colorectal cancer; therapeutic targets; biomarkers},
   language = {eng},
   title = {Genes of the microRNA biogenesis pathway are deregulated in Colorectal cancer},
   year = {2013}
}

TY  - CONF
ID  - 1128521
AU  - Slabý, Ondřej - Vychytilová, Petra - Svoboda, Marek - Vyzula, Rostislav
PY  - 2013
TI  - Genes of the microRNA biogenesis pathway are deregulated in Colorectal cancer
KW  - microRNA
KW  - biogenesis
KW  - colorectal cancer
KW  - therapeutic targets
KW  - biomarkers
N2  - MicroRNAs (miRNAs) are small non-coding RNAs 18-25 nt long that regulate gene expression on post-transcriptional level. Therefore, their production and maturation have to be strictly regulated. Their biogenesis starts with the transcription, which is followed by several steps that lead to processing of primary transcripts to mature miRNAs. Each step of this pathway is sophistically regulated and any disruption of control mechanisms may leads to the cancer occurrence. The aim of this study was to analyse the expression of the crucial genes involved in the biogenesis of miRNAs in colorectal cancer (CRC). Expression of 19 selected genes has been analysed in tumor tissues of 120 clinically characterized patients with CRC and in 120 parallel healthy tissues by qRT-PCR. Using Wilcoxon test, genes with different expression between tumor and healthy tissue have been identified. Subsequently, Kruskal-Wallis test has been used to find any correlation with the clinical-pathological features of the patients. We have found significantly higher expression of POLRIIA, ADAR, ADARB1, DGCR8, DDX5, DDX17, DDX20, DROSHA, XPO5, EIF2C1-C4, TARBP2, TNRC6A, GEMIN3, and DICER1 (P < 0.0001) and significantly lower expression of LIN28A in tumor tissue of CRC patients. Moreover, negative correlation between the expression of EIF2C3 and clinical stage of patients (P = 0.0017) and grade (P = 0.0151) and positive correlation between the expression of DICER1 (P = 0.0230) and DROSHA (P = 0.0212) and grade of patients has been observed. Our results show that changes in the expression of genes associated with biogenesis of miRNAs may be associated not only with the origin, but also with the progression of CRC and therefore, these molecules could serve as potential new biomarkers or therapeutic targets.
ER  -

SLABÝ, Ondřej; Petra VYCHYTILOVÁ; Marek SVOBODA a Rostislav VYZULA. Genes of the microRNA biogenesis pathway are deregulated in Colorectal cancer. In \textit{3rd World Congress on Cancer Science and Therapy, CA, USA}. 2013. ISSN~1948-5956.

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