Physicochemical analysis of rotavirus segment 11 supports a
‘modified panhandle’ structure and not the predicted
alternative tRNA-like structure (TRLS)

J 2013

Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS)

BISWAS, Subhajit; Wilson LI; Emily MANKTELOW; Jonathan LEVER; Laura E. EASTON et. al.

Základní údaje

Originální název

Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS)

Autoři

BISWAS, Subhajit; Wilson LI; Emily MANKTELOW; Jonathan LEVER; Laura E. EASTON; Peter LUKAVSKY; Ulrich DESSELBERGER a Andrew M LEVER

Vydání

ARCHIVES OF VIROLOGY, Vídeň, SPRINGER WIEN, 2013, 0304-8608

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.282

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1007/s00705-013-1802-8

UT WoS

000330960200006

Klíčová slova anglicky

rotavirus; tRNA-like structure; RNA 11

Štítky

neMU, neRIV, ok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 12. 2013 14:07, Olga Křížová

Anotace

V originále

Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements. Covariation studies and phylogenetic analysis exploring the potential structure of RNA11 of rotaviruses suggested that, besides the previously predicted ‘‘modified panhandle’’ structure, the 5’ and 3’ termini of one of the isoforms of the bovine rotavirus UKtc strain may interact to form a tRNA-like structure (TRLS). Such TRLSs have been identified in RNAs of plant viruses, where they are important for enhancing replication and packaging. However, using tRNA mimicry assays (in vitro aminoacylation and 3’- adenylation), we found no biochemical evidence for tRNA-like functions of RNA11. Capping, synthetic 3’ adenylation and manipulation of divalent cation concentrations did not change this finding. NMR studies on a 5’- and 3’-deletion construct of RNA11 containing the putative intra-strand complementary sequences supported a predominant panhandle structure and did not conform to a cloverleaf fold despite the strong evidence for a predicted structure in this conserved region of the viral RNA. Additional viral or cellular factors may be needed to stabilise it into a form with tRNA-like properties

Citovat

BISWAS, Subhajit; Wilson LI; Emily MANKTELOW; Jonathan LEVER; Laura E. EASTON; Peter LUKAVSKY; Ulrich DESSELBERGER a Andrew M LEVER. Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS). ARCHIVES OF VIROLOGY. Vídeň: SPRINGER WIEN, 2013, Neuveden, č. 8, s. "nestránkováno", 14 s. ISSN 0304-8608. Dostupné z: https://doi.org/10.1007/s00705-013-1802-8.

@article{1136053,
   author = {Biswas, Subhajit and Li, Wilson and Manktelow, Emily and Lever, Jonathan and Easton, Laura E. and Lukavsky, Peter and Desselberger, Ulrich and Lever, Andrew M},
   article_location = {Vídeň},
   article_number = {8},
   doi = {https://doi.org/10.1007/s00705-013-1802-8},
   keywords = {rotavirus; tRNA-like structure; RNA 11},
   language = {eng},
   issn = {0304-8608},
   journal = {ARCHIVES OF VIROLOGY},
   title = {Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS)},
   url = {http://www.readcube.com/articles/10.1007/s00705-013-1802-8?locale=en},
   volume = {Neuveden},
   year = {2013}
}

TY  - JOUR
ID  - 1136053
AU  - Biswas, Subhajit - Li, Wilson - Manktelow, Emily - Lever, Jonathan - Easton, Laura E. - Lukavsky, Peter - Desselberger, Ulrich - Lever, Andrew M
PY  - 2013
TI  - Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS)
JF  - ARCHIVES OF VIROLOGY
VL  - Neuveden
IS  - 8
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - SPRINGER WIEN
SN  - 03048608
KW  - rotavirus
KW  - tRNA-like structure
KW  - RNA 11
UR  - http://www.readcube.com/articles/10.1007/s00705-013-1802-8?locale=en
N2  - Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements. Covariation studies and phylogenetic analysis exploring the potential structure of RNA11 of rotaviruses suggested that, besides the previously predicted ‘‘modified panhandle’’ structure, the 5’ and 3’ termini of one of the isoforms of the bovine rotavirus UKtc strain may interact to form a tRNA-like structure (TRLS). Such TRLSs have been identified in RNAs of plant viruses, where they are important for enhancing replication and packaging. However, using tRNA mimicry assays (in vitro aminoacylation and 3’- adenylation), we found no biochemical evidence for tRNA-like functions of RNA11. Capping, synthetic 3’ adenylation and manipulation of divalent cation concentrations did not change this finding. NMR studies on a 5’- and 3’-deletion construct of RNA11 containing the putative intra-strand complementary sequences supported a predominant panhandle structure and did not conform to a cloverleaf fold despite the strong evidence for a predicted structure in this conserved region of the viral RNA. Additional viral or cellular factors may be needed to stabilise it into a form with tRNA-like properties
ER  -

BISWAS, Subhajit; Wilson LI; Emily MANKTELOW; Jonathan LEVER; Laura E. EASTON; Peter LUKAVSKY; Ulrich DESSELBERGER a Andrew M LEVER. Physicochemical analysis of rotavirus segment 11 supports a ‘modified panhandle’ structure and not the predicted alternative tRNA-like structure (TRLS). \textit{ARCHIVES OF VIROLOGY}. Vídeň: SPRINGER WIEN, 2013, Neuveden, č.~8, s.~''nestránkováno'', 14 s. ISSN~0304-8608. Dostupné z: https://doi.org/10.1007/s00705-013-1802-8.

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