Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles
with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial
Agents for Vascular Grafts

J 2013

Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial Agents for Vascular Grafts

CHUDOBOVA, Dagmar; Lukas NEJDL; Jaromír GUMULEC; Olga KRYSTOFOVA; Miguel A M RODRIGO et al.

Základní údaje

Originální název

Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial Agents for Vascular Grafts

Autoři

CHUDOBOVA, Dagmar; Lukas NEJDL; Jaromír GUMULEC; Olga KRYSTOFOVA; Miguel A M RODRIGO; Jindřich KYNICKÝ; Branislav RUTTKAY-NEDECKY; Pavel KOPEL; Petr BABULA; Vojtech ADAM a René KIZEK

Vydání

International Journal of Molecular Sciences, 2013, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/13:00070460

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.3390/ijms140713592

UT WoS

000322171700041

Klíčová slova anglicky

polymers; antimicrobial activity; silver ions; silver phosphate nanoparticles; hyaluronic acid; chitosan

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2014 12:15, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate or silver phosphate nanoparticles were created, and their effects on gram-positive bacterial culture of Staphylococcus aureus were monitored. Stages of formation of complexes of silver nitrate and silver phosphate nanoparticles with polymeric compounds were characterized using electrochemical and spectrophotometric methods. Furthermore, the antimicrobial activity of complexes was determined using the methods of determination of growth curves and zones of inhibition. The results of this study revealed that the complex of chitosan, with silver phosphate nanoparticles, was the most suitable in order to have an antibacterial effect on bacterial culture of Staphylococcus aureus. Formation of this complex was under way at low concentrations of chitosan. The results of electrochemical determination corresponded with the results of spectrophotometric methods and verified good interaction and formation of the complex. The complex has an outstanding antibacterial effect and this effect was of several orders higher compared to other investigated complexes.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Přiložené soubory

Chudobova_et_al_2013_IJMS.pdf Verze souboru

Citovat

CHUDOBOVA, Dagmar; Lukas NEJDL; Jaromír GUMULEC; Olga KRYSTOFOVA; Miguel A M RODRIGO; Jindřich KYNICKÝ; Branislav RUTTKAY-NEDECKY; Pavel KOPEL; Petr BABULA; Vojtech ADAM a René KIZEK. Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial Agents for Vascular Grafts. International Journal of Molecular Sciences. 2013, roč. 14, č. 7, s. 13592-13614. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms140713592.

@article{1136059,
   author = {Chudobova, Dagmar and Nejdl, Lukas and Gumulec, Jaromír and Krystofova, Olga and Rodrigo, Miguel A M and Kynický, Jindřich and RuttkayandNedecky, Branislav and Kopel, Pavel and Babula, Petr and Adam, Vojtech and Kizek, René},
   article_number = {7},
   doi = {https://doi.org/10.3390/ijms140713592},
   keywords = {polymers; antimicrobial activity; silver ions; silver phosphate nanoparticles; hyaluronic acid; chitosan},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial Agents for Vascular Grafts},
   url = {http://www.mdpi.com/1422-0067/14/7/13592},
   volume = {14},
   year = {2013}
}

TY  - JOUR
ID  - 1136059
AU  - Chudobova, Dagmar - Nejdl, Lukas - Gumulec, Jaromír - Krystofova, Olga - Rodrigo, Miguel A M - Kynický, Jindřich - Ruttkay-Nedecky, Branislav - Kopel, Pavel - Babula, Petr - Adam, Vojtech - Kizek, René
PY  - 2013
TI  - Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial Agents for Vascular Grafts
JF  - International Journal of Molecular Sciences
VL  - 14
IS  - 7
SP  - 13592-13614
EP  - 13592-13614
SN  - 14220067
KW  - polymers
KW  - antimicrobial activity
KW  - silver ions
KW  - silver phosphate nanoparticles
KW  - hyaluronic acid
KW  - chitosan
UR  - http://www.mdpi.com/1422-0067/14/7/13592
N2  - Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate or silver phosphate nanoparticles were created, and their effects on gram-positive bacterial culture of Staphylococcus aureus were monitored. Stages of formation of complexes of silver nitrate and silver phosphate nanoparticles with polymeric compounds were characterized using electrochemical and spectrophotometric methods. Furthermore, the antimicrobial activity of complexes was determined using the methods of determination of growth curves and zones of inhibition. The results of this study revealed that the complex of chitosan, with silver phosphate nanoparticles, was the most suitable in order to have an antibacterial effect on bacterial culture of Staphylococcus aureus. Formation of this complex was under way at low concentrations of chitosan. The results of electrochemical determination corresponded with the results of spectrophotometric methods and verified good interaction and formation of the complex. The complex has an outstanding antibacterial effect and this effect was of several orders higher compared to other investigated complexes.
ER  -

CHUDOBOVA, Dagmar; Lukas NEJDL; Jaromír GUMULEC; Olga KRYSTOFOVA; Miguel A M RODRIGO; Jindřich KYNICKÝ; Branislav RUTTKAY-NEDECKY; Pavel KOPEL; Petr BABULA; Vojtech ADAM a René KIZEK. Complexes of Silver(I) Ions and Silver Phosphate Nanoparticles with Hyaluronic Acid and/or Chitosan as Promising Antimicrobial Agents for Vascular Grafts. \textit{International Journal of Molecular Sciences}. 2013, roč.~14, č.~7, s.~13592-13614. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms140713592.

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