Expression of Bcl-2 and other regulatory genes in primary cell
culture derived from spinocellular tumour of head and neck

a 2013

Expression of Bcl-2 and other regulatory genes in primary cell culture derived from spinocellular tumour of head and neck

GUMULEC, Jaromír; Markéta SZTALMACHOVÁ; Hana POLANSKÁ; Monika HOLUBOVÁ; Jan BALVAN et al.

Základní údaje

Originální název

Expression of Bcl-2 and other regulatory genes in primary cell culture derived from spinocellular tumour of head and neck

Autoři

Vydání

The 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine, 2013

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.880

Označené pro přenos do RIV

ISSN

UT WoS

000324507400172

Změněno: 9. 1. 2014 10:13, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Relative lack of symptoms of head and neck tumours together with limited effect of treatment of advanced stages highlights the need of novel cancer biomarkers. Consistently with the identification of such molecules, understanding of molecular mechanisms of head and neck tumours is needed. Therefore, we prepared a primary cell culture from advanced spinocellular pharyngeal tumour and analysed the expression of apoptosis- and cell cycle-related genes. Following genes were analysed: Bax, Bcl-2, p53. The expression of these genes was analysed on qRT-PCR using Taqman probes and relative ddCt method, and standardized to beta-actin as a housekeeping gene. To eliminate the effect of fibroblast overgrowing, medium with D-valin was used. Non-tumour tissue (not treated with D-valin) was used as a relative control. Additionally to qRT-PCR, cell growth was analysed using impedance-based real-time cell growth monitorring system. In this pilot study, we determined significant differences in the expression of both Bax and Bcl-2 and no significant difference in the expression of p53 gene (p < 0.05, t-test). These preliminary data suggest this particular cell culture of head and neck utilize inhibition of apoptosis as a way to promote tumorigenesis. regulation is distinctly affected in this primary cell culture. As a result of modification of these processes, differential growth of tumorous and non-tumour primary cell culture was determined. However, further experiments focused on the characterisation of this cellular culture are needed.

Návaznosti

NT14337, projekt VaV

Název: Studium a charakterizace primárních nádorových buněčných linií spinocelulárních karcinomů v oblasti hlavy a krku a jejich maligní potenciál.

Investor: Ministerstvo zdravotnictví ČR, Studium a charakterizace primárních nádorových buněčných linií spinocelulárních karcinomů v oblasti hlavy a krku a jejich maligní potenciál.

Citovat

GUMULEC, Jaromír; Markéta SZTALMACHOVÁ; Hana POLANSKÁ; Monika HOLUBOVÁ; Jan BALVAN; Kristýna HUDCOVÁ; Hana BINKOVÁ; Zuzana HORÁKOVÁ; Rom KOSTŘICA; Martina RAUDENSKÁ; Ondřej ZÍTKA; Vojtěch ADAM; René KIZEK a Michal MASAŘÍK. Expression of Bcl-2 and other regulatory genes in primary cell culture derived from spinocellular tumour of head and neck. In The 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine. 2013. ISSN 1107-3756.

@proceedings{1136284,
   author = {Gumulec, Jaromír and Sztalmachová, Markéta and Polanská, Hana and Holubová, Monika and Balvan, Jan and Hudcová, Kristýna and Binková, Hana and Horáková, Zuzana and Kostřica, Rom and Raudenská, Martina and Zítka, Ondřej and Adam, Vojtěch and Kizek, René and Masařík, Michal},
   booktitle = {The 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine},
   title = {Expression of Bcl-2 and other regulatory genes in primary cell culture derived from spinocellular tumour of head and neck},
   year = {2013}
}

TY  - CONF
ID  - 1136284
AU  - Gumulec, Jaromír - Sztalmachová, Markéta - Polanská, Hana - Holubová, Monika - Balvan, Jan - Hudcová, Kristýna - Binková, Hana - Horáková, Zuzana - Kostřica, Rom - Raudenská, Martina - Zítka, Ondřej - Adam, Vojtěch - Kizek, René - Masařík, Michal
PY  - 2013
TI  - Expression of Bcl-2 and other regulatory genes in primary cell culture derived from spinocellular tumour of head and neck
N2  - Relative lack of symptoms of head and neck tumours together with limited effect of treatment of advanced stages highlights the need of novel cancer biomarkers. Consistently with the identification of such molecules, understanding of molecular mechanisms of head and neck tumours is needed. Therefore, we prepared a primary cell culture from advanced spinocellular pharyngeal tumour and analysed the expression of apoptosis- and cell cycle-related genes. Following genes were analysed: Bax, Bcl-2, p53. The expression of these genes was analysed on qRT-PCR using Taqman probes and relative ddCt method, and standardized to beta-actin as a housekeeping gene. To eliminate the effect of fibroblast overgrowing, medium with D-valin was used. Non-tumour tissue (not treated with D-valin) was used as a relative control. Additionally to qRT-PCR, cell growth was analysed using impedance-based real-time cell growth monitorring system. In this pilot study, we determined significant differences in the expression of both Bax and Bcl-2 and no significant difference in the expression of p53 gene (p < 0.05, t-test). These preliminary data suggest this particular cell culture of head and neck utilize inhibition of apoptosis as a way to promote tumorigenesis. regulation is distinctly affected in this primary cell culture. As a result of modification of these processes, differential growth of tumorous and non-tumour primary cell culture was determined. However, further experiments focused on the characterisation of this cellular culture are needed.
ER  -

GUMULEC, Jaromír; Markéta SZTALMACHOVÁ; Hana POLANSKÁ; Monika HOLUBOVÁ; Jan BALVAN; Kristýna HUDCOVÁ; Hana BINKOVÁ; Zuzana HORÁKOVÁ; Rom KOSTŘICA; Martina RAUDENSKÁ; Ondřej ZÍTKA; Vojtěch ADAM; René KIZEK a Michal MASAŘÍK. Expression of Bcl-2 and other regulatory genes in primary cell culture derived from spinocellular tumour of head and neck. In \textit{The 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine}. 2013. ISSN~1107-3756.

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