Crystallographic Analysis of New Psychrophilic Haloalkane
Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and
DmxA from Marinobacter sp. ELB17

J 2013

Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

TRATSIAK, K.; O. DEGTJARIK; I. DRIENOVSKA; Lukáš CHRÁST; P. REZACOVA et al.

Základní údaje

Originální název

Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17

Autoři

TRATSIAK, K.; O. DEGTJARIK; I. DRIENOVSKA; Lukáš CHRÁST; P. REZACOVA; M. KUTY; Radka CHALOUPKOVÁ; Jiří DAMBORSKÝ a I. KUTA SMATANOVA

Vydání

Acta Crystallographica, 2013, 1744-3091

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.568

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00066615

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1107/S1744309113012979

UT WoS

000319736000023

Klíčová slova anglicky

Haloalkane Dehalogenases

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 4. 2014 16:17, Ing. Andrea Mikešková

Anotace

V originále

Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

GAP207/12/0775, projekt VaV

Název: Strukturně-funkční vztahy haloalkan dehalogenas

Investor: Grantová agentura ČR, Structure-functional Relationships of Haloalkane Dehalogenases

Citovat

TRATSIAK, K.; O. DEGTJARIK; I. DRIENOVSKA; Lukáš CHRÁST; P. REZACOVA; M. KUTY; Radka CHALOUPKOVÁ; Jiří DAMBORSKÝ a I. KUTA SMATANOVA. Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. Acta Crystallographica. 2013, roč. 2013, F69, s. 683-688. ISSN 1744-3091. Dostupné z: https://doi.org/10.1107/S1744309113012979.

@article{1137344,
   author = {Tratsiak, K. and Degtjarik, O. and Drienovska, I. and Chrást, Lukáš and Rezacova, P. and Kuty, M. and Chaloupková, Radka and Damborský, Jiří and Kuta Smatanova, I.},
   article_number = {F69},
   doi = {https://doi.org/10.1107/S1744309113012979},
   keywords = {Haloalkane Dehalogenases},
   language = {eng},
   issn = {1744-3091},
   journal = {Acta Crystallographica},
   title = {Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17},
   volume = {2013},
   year = {2013}
}

TY  - JOUR
ID  - 1137344
AU  - Tratsiak, K. - Degtjarik, O. - Drienovska, I. - Chrást, Lukáš - Rezacova, P. - Kuty, M. - Chaloupková, Radka - Damborský, Jiří - Kuta Smatanova, I.
PY  - 2013
TI  - Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17
JF  - Acta Crystallographica
VL  - 2013
IS  - F69
SP  - 683-688
EP  - 683-688
SN  - 17443091
KW  - Haloalkane Dehalogenases
N2  - Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A.
ER  -

TRATSIAK, K.; O. DEGTJARIK; I. DRIENOVSKA; Lukáš CHRÁST; P. REZACOVA; M. KUTY; Radka CHALOUPKOVÁ; Jiří DAMBORSKÝ a I. KUTA SMATANOVA. Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. \textit{Acta Crystallographica}. 2013, roč.~2013, F69, s.~683-688. ISSN~1744-3091. Dostupné z: https://doi.org/10.1107/S1744309113012979.

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