| TRATSIAK, K., O. DEGTJARIK, I. DRIENOVSKA, Lukáš CHRÁST, P. REZACOVA, M. KUTY, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ a I. KUTA SMATANOVA. Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. Acta Crystallographica. 2013, roč. 2013, F69, s. 683-688. ISSN 1744-3091. Dostupné z: https://dx.doi.org/10.1107/S1744309113012979. |
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@article{1137344,
author = {Tratsiak, K. and Degtjarik, O. and Drienovska, I. and Chrást, Lukáš and Rezacova, P. and Kuty, M. and Chaloupková, Radka and Damborský, Jiří and Kuta Smatanova, I.},
article_number = {F69},
doi = {http://dx.doi.org/10.1107/S1744309113012979},
keywords = {Haloalkane Dehalogenases},
language = {eng},
issn = {1744-3091},
journal = {Acta Crystallographica},
title = {Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17},
volume = {2013},
year = {2013}
}
TY - JOUR
ID - 1137344
AU - Tratsiak, K. - Degtjarik, O. - Drienovska, I. - Chrást, Lukáš - Rezacova, P. - Kuty, M. - Chaloupková, Radka - Damborský, Jiří - Kuta Smatanova, I.
PY - 2013
TI - Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17
JF - Acta Crystallographica
VL - 2013
IS - F69
SP - 683-688
EP - 683-688
SN - 17443091
KW - Haloalkane Dehalogenases
N2 - Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 A.
ER -
TRATSIAK, K., O. DEGTJARIK, I. DRIENOVSKA, Lukáš CHRÁST, P. REZACOVA, M. KUTY, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ a I. KUTA SMATANOVA. Crystallographic Analysis of New Psychrophilic Haloalkane Dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17. \textit{Acta Crystallographica}. 2013, roč.~2013, F69, s.~683-688. ISSN~1744-3091. Dostupné z: https://dx.doi.org/10.1107/S1744309113012979.
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