Improvement of MALDI-TOF MS profiling for the differentiation
of species within the Acinetobacter calcoaceticus-Acinetobacter
baumannii complex

J 2013

Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex

ŠEDO, Ondrej; Alexandr NEMEC; Lenka KŘÍŽOVÁ; Magdaléna KAČALOVÁ; Zbyněk ZDRÁHAL et. al.

Základní údaje

Originální název

Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex

Autoři

ŠEDO, Ondrej (203 Česká republika, domácí); Alexandr NEMEC (203 Česká republika); Lenka KŘÍŽOVÁ (203 Česká republika); Magdaléna KAČALOVÁ (203 Česká republika, domácí) a Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí)

Vydání

Systematic and Applied Microbiology, Jena, Elsevier, Urban & Fischer Verlag, 2013, 0723-2020

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.310

Kód RIV

RIV/00216224:14740/13:00066649

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.syapm.2013.08.001

UT WoS

000328176300007

Klíčová slova anglicky

MALDI-TOF MS; Acinetobacter calcoaceticus-Acinetobacter baumannii complex; Bacteria profiling; BioTyper

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 4. 2014 04:12, Olga Křížová

Anotace

V originále

MALDI-TOF MS is currently becoming the method of choice for rapid identification of bacterial species. in routine diagnostics. Yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, namely A. baumannii and Acinetobacter nosocomialis. In the present study, We evaluated a protocol which was different from that used in the Bruker Daltonics identification system (MALDI BioTyper) to improve species identification using a taxonomically precisely defined set of 105 strains representing the four validly named species of the ACB complex. The novel protocol is based on the change in matrix composition from alpha-cyano-4-hydroxycinnamic acid (saturated solution in water:acetonitrile:trifluoroacetic acid, 47.5:50:2.5, v/v) to ferulic acid (12.5 mg ml(-1) solution in water:acetonitrile:formic acid 50:33:17, v/v), while the other steps of sample processing remain unchanged. Compared to the standard protocol, the novel one extended the range of detected compounds towards higher molecular weight, produced signals with better mass resolution, and allowed the detection of species-specific signals. As a result, differentiation of A. nosocomialis and A. baumannii strains by cluster analysis was improved and 13 A. nosocomialis strains, assigned erroneously or ambiguously by using the standard protocol, were correctly identified.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA13-26693S, projekt VaV

Název: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.

Investor: Grantová agentura ČR, Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

ŠEDO, Ondrej; Alexandr NEMEC; Lenka KŘÍŽOVÁ; Magdaléna KAČALOVÁ a Zbyněk ZDRÁHAL. Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Systematic and Applied Microbiology. Jena: Elsevier, Urban & Fischer Verlag, 2013, roč. 36, č. 8, s. 572-578. ISSN 0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2013.08.001.

@article{1138415,
   author = {Šedo, Ondrej and Nemec, Alexandr and Křížová, Lenka and Kačalová, Magdaléna and Zdráhal, Zbyněk},
   article_location = {Jena},
   article_number = {8},
   doi = {http://dx.doi.org/10.1016/j.syapm.2013.08.001},
   keywords = {MALDI-TOF MS; Acinetobacter calcoaceticus-Acinetobacter baumannii complex; Bacteria profiling; BioTyper},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex},
   volume = {36},
   year = {2013}
}

TY  - JOUR
ID  - 1138415
AU  - Šedo, Ondrej - Nemec, Alexandr - Křížová, Lenka - Kačalová, Magdaléna - Zdráhal, Zbyněk
PY  - 2013
TI  - Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex
JF  - Systematic and Applied Microbiology
VL  - 36
IS  - 8
SP  - 572-578
EP  - 572-578
PB  - Elsevier, Urban & Fischer Verlag
SN  - 07232020
KW  - MALDI-TOF MS
KW  - Acinetobacter calcoaceticus-Acinetobacter baumannii complex
KW  - Bacteria profiling
KW  - BioTyper
N2  - MALDI-TOF MS is currently becoming the method of choice for rapid identification of bacterial species. in routine diagnostics. Yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, namely A. baumannii and Acinetobacter nosocomialis. In the present study, We evaluated a protocol which was different from that used in the Bruker Daltonics identification system (MALDI BioTyper) to improve species identification using a taxonomically precisely defined set of 105 strains representing the four validly named species of the ACB complex. The novel protocol is based on the change in matrix composition from alpha-cyano-4-hydroxycinnamic acid (saturated solution in water:acetonitrile:trifluoroacetic acid, 47.5:50:2.5, v/v) to ferulic acid (12.5 mg ml(-1) solution in water:acetonitrile:formic acid 50:33:17, v/v), while the other steps of sample processing remain unchanged. Compared to the standard protocol, the novel one extended the range of detected compounds towards higher molecular weight, produced signals with better mass resolution, and allowed the detection of species-specific signals. As a result, differentiation of A. nosocomialis and A. baumannii strains by cluster analysis was improved and 13 A. nosocomialis strains, assigned erroneously or ambiguously by using the standard protocol, were correctly identified.
ER  -

ŠEDO, Ondrej; Alexandr NEMEC; Lenka KŘÍŽOVÁ; Magdaléna KAČALOVÁ a Zbyněk ZDRÁHAL. Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. \textit{Systematic and Applied Microbiology}. Jena: Elsevier, Urban \&{} Fischer Verlag, 2013, roč.~36, č.~8, s.~572-578. ISSN~0723-2020. Dostupné z: https://dx.doi.org/10.1016/j.syapm.2013.08.001.

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