Improvement of MALDI-TOF MS profiling for the differentiation
of species within the Acinetobacter calcoaceticus-Acinetobacter
baumannii complex

J 2013

Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex

ŠEDO, Ondrej; Alexandr NEMEC; Lenka KŘÍŽOVÁ; Magdaléna KAČALOVÁ; Zbyněk ZDRÁHAL et. al.

Basic information

Original name

Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex

Authors

ŠEDO, Ondrej (203 Czech Republic, belonging to the institution); Alexandr NEMEC (203 Czech Republic); Lenka KŘÍŽOVÁ (203 Czech Republic); Magdaléna KAČALOVÁ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Systematic and Applied Microbiology, Jena, Elsevier, Urban & Fischer Verlag, 2013, 0723-2020

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 3.310

RIV identification code

RIV/00216224:14740/13:00066649

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.syapm.2013.08.001

UT WoS

000328176300007

Keywords in English

MALDI-TOF MS; Acinetobacter calcoaceticus-Acinetobacter baumannii complex; Bacteria profiling; BioTyper

Abstract

In the original language

MALDI-TOF MS is currently becoming the method of choice for rapid identification of bacterial species. in routine diagnostics. Yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, namely A. baumannii and Acinetobacter nosocomialis. In the present study, We evaluated a protocol which was different from that used in the Bruker Daltonics identification system (MALDI BioTyper) to improve species identification using a taxonomically precisely defined set of 105 strains representing the four validly named species of the ACB complex. The novel protocol is based on the change in matrix composition from alpha-cyano-4-hydroxycinnamic acid (saturated solution in water:acetonitrile:trifluoroacetic acid, 47.5:50:2.5, v/v) to ferulic acid (12.5 mg ml(-1) solution in water:acetonitrile:formic acid 50:33:17, v/v), while the other steps of sample processing remain unchanged. Compared to the standard protocol, the novel one extended the range of detected compounds towards higher molecular weight, produced signals with better mass resolution, and allowed the detection of species-specific signals. As a result, differentiation of A. nosocomialis and A. baumannii strains by cluster analysis was improved and 13 A. nosocomialis strains, assigned erroneously or ambiguously by using the standard protocol, were correctly identified.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-26693S, research and development project

Name: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.

Investor: Czech Science Foundation

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Cite

ŠEDO, Ondrej; Alexandr NEMEC; Lenka KŘÍŽOVÁ; Magdaléna KAČALOVÁ and Zbyněk ZDRÁHAL. Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Systematic and Applied Microbiology. Jena: Elsevier, Urban & Fischer Verlag, 2013, vol. 36, No 8, p. 572-578. ISSN 0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2013.08.001.

@article{1138415,
   author = {Šedo, Ondrej and Nemec, Alexandr and Křížová, Lenka and Kačalová, Magdaléna and Zdráhal, Zbyněk},
   article_location = {Jena},
   article_number = {8},
   doi = {http://dx.doi.org/10.1016/j.syapm.2013.08.001},
   keywords = {MALDI-TOF MS; Acinetobacter calcoaceticus-Acinetobacter baumannii complex; Bacteria profiling; BioTyper},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex},
   volume = {36},
   year = {2013}
}

TY  - JOUR
ID  - 1138415
AU  - Šedo, Ondrej - Nemec, Alexandr - Křížová, Lenka - Kačalová, Magdaléna - Zdráhal, Zbyněk
PY  - 2013
TI  - Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex
JF  - Systematic and Applied Microbiology
VL  - 36
IS  - 8
SP  - 572-578
EP  - 572-578
PB  - Elsevier, Urban & Fischer Verlag
SN  - 07232020
KW  - MALDI-TOF MS
KW  - Acinetobacter calcoaceticus-Acinetobacter baumannii complex
KW  - Bacteria profiling
KW  - BioTyper
N2  - MALDI-TOF MS is currently becoming the method of choice for rapid identification of bacterial species. in routine diagnostics. Yet, this method suffers from the inability to differentiate reliably between some closely related bacterial species including those of the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex, namely A. baumannii and Acinetobacter nosocomialis. In the present study, We evaluated a protocol which was different from that used in the Bruker Daltonics identification system (MALDI BioTyper) to improve species identification using a taxonomically precisely defined set of 105 strains representing the four validly named species of the ACB complex. The novel protocol is based on the change in matrix composition from alpha-cyano-4-hydroxycinnamic acid (saturated solution in water:acetonitrile:trifluoroacetic acid, 47.5:50:2.5, v/v) to ferulic acid (12.5 mg ml(-1) solution in water:acetonitrile:formic acid 50:33:17, v/v), while the other steps of sample processing remain unchanged. Compared to the standard protocol, the novel one extended the range of detected compounds towards higher molecular weight, produced signals with better mass resolution, and allowed the detection of species-specific signals. As a result, differentiation of A. nosocomialis and A. baumannii strains by cluster analysis was improved and 13 A. nosocomialis strains, assigned erroneously or ambiguously by using the standard protocol, were correctly identified.
ER  -

ŠEDO, Ondrej; Alexandr NEMEC; Lenka KŘÍŽOVÁ; Magdaléna KAČALOVÁ and Zbyněk ZDRÁHAL. Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. \textit{Systematic and Applied Microbiology}. Jena: Elsevier, Urban \&{} Fischer Verlag, 2013, vol.~36, No~8, p.~572-578. ISSN~0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2013.08.001.

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