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@article{1138483,
author = {Frankova, Jana and Pivodová, Veronika and Růžička, Filip and Tománková, Kateřina and Šafářová, Klára and Vrbková, Jana and Ulrichová, Jitka},
article_location = {HOBOKEN},
article_number = {10},
doi = {http://dx.doi.org/10.1002/jbm.a.34598},
keywords = {titanium modification; bacterial strains; gingival fibroblasts; adhesion},
language = {eng},
issn = {1549-3296},
journal = {JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A},
title = {Comparing biocompatibility of gingival fibroblasts and bacterial strains on a different modified titanium discs},
volume = {101},
year = {2013}
}
TY - JOUR
ID - 1138483
AU - Frankova, Jana - Pivodová, Veronika - Růžička, Filip - Tománková, Kateřina - Šafářová, Klára - Vrbková, Jana - Ulrichová, Jitka
PY - 2013
TI - Comparing biocompatibility of gingival fibroblasts and bacterial strains on a different modified titanium discs
JF - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
VL - 101
IS - 10
SP - 2915-2924
EP - 2915-2924
PB - WILEY-BLACKWELL
SN - 15493296
KW - titanium modification
KW - bacterial strains
KW - gingival fibroblasts
KW - adhesion
N2 - The modification of implant surface situated in the area of peri-implant sulcus has important role in bacterial and cell adhesion. Six different chemically and physically modified titanium discs were prepared: glazed (Tis-MALP), unglazed (Tis-O), unglazed and alkali-etched (Tis-OA), unglazed and coated with ZrN (Tis-OZ), unglazed, sand blasted, and acid etched (Tis-OPAE), and unglazed, sand blasted, acid, and alkali etched (Tis-OPAAE). Analysis of surface topography was determined using scanning electron microscopy and atomic force microscopy (AFM). Biocompatibility of gingival fibroblasts was characterized by the production of tumor necrosis factor alpha, collagen I, matrix metalloproteinase 2 (MMP-2) after 24 and 72 h and expression of (31) integrin and vinculin using enzyme-linked immunosorbent assay (ELISA) or modified ELISA after 6 and 24 h. Microorganism adhesion (five bacterial strains) and biofilm formation was also evaluated. The adhesion of bacteria and gingival fibroblasts was significantly higher on titanium disc Tis-OPAAE and biofilm formation on the same surface for Streptococcus mutans, Streptococcus gordonii, and Streptococcus intermedius. The gingival fibroblasts on Tis-OPAAE disc had also significantly lower production of MMP-2. The collagen production was significantly lower on all surfaces with roughness higher than 0.2 m. This study confirmed that the titanium disc with the surface roughness 3.39 m (Tis-OPAAE) supported the adhesion of bacterial strains as well as gingival fibroblasts.
ER -
FRANKOVA, Jana, Veronika PIVODOVÁ, Filip RŮŽIČKA, Kateřina TOMÁNKOVÁ, Klára ŠAFÁŘOVÁ, Jana VRBKOVÁ a Jitka ULRICHOVÁ. Comparing biocompatibility of gingival fibroblasts and bacterial strains on a different modified titanium discs. \textit{JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A}. HOBOKEN: WILEY-BLACKWELL, 2013, roč.~101, č.~10, s.~2915-2924. ISSN~1549-3296. Dostupné z: https://dx.doi.org/10.1002/jbm.a.34598.
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