Combination of micropreparative solution isoelectric focusing
and high-performance liquid chromatography for differentiation
of biofilm-positive and biofilm-negative Candida parapsilosis
group from vascular catheter

J 2014

Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter

VYKYDALOVÁ, Marie; Marie HORKÁ; Filip RŮŽIČKA; Filip DUŠA; Dana MORAVCOVÁ et al.

Základní údaje

Originální název

Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter

Autoři

VYKYDALOVÁ, Marie; Marie HORKÁ; Filip RŮŽIČKA ; Filip DUŠA; Dana MORAVCOVÁ; Vladislav KAHLE a Karel ŠLAIS

Vydání

Analytica Chimica Acta, AMSTERDAM, ELSEVIER SCIENCE BV, 2014, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 4.513

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/14:00073211

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Candida parapsilosis group; biofilm; micropreparative solution isoelectric focusing; high-performance liquid chromatography; capillary isoelectric focusing; sonicate from vascular catheter

Štítky

EL OK, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 1. 2020 17:19, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material – sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto.

Návaznosti

VG20112015021, projekt VaV

Název: Vývoj instrumentálních metodických postupů rychlé detekce a identifikace biologických agens v reálných vzorcích (Akronym: Detekce a identifikace biol. agens)

Investor: Ministerstvo vnitra ČR, Vývoj instrumentálních metodických postupů rychlé detekce a identifikace biologických agens v reálných vzorcích

Citovat

VYKYDALOVÁ, Marie; Marie HORKÁ; Filip RŮŽIČKA; Filip DUŠA; Dana MORAVCOVÁ; Vladislav KAHLE a Karel ŠLAIS. Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter. Analytica Chimica Acta. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč. 812, FEB, s. 243-249. ISSN 0003-2670. Dostupné z: https://doi.org/10.1016/j.aca.2013.12.032.

@article{1138634,
   author = {Vykydalová, Marie and Horká, Marie and Růžička, Filip and Duša, Filip and Moravcová, Dana and Kahle, Vladislav and Šlais, Karel},
   article_location = {AMSTERDAM},
   article_number = {FEB},
   doi = {https://doi.org/10.1016/j.aca.2013.12.032},
   keywords = {Candida parapsilosis group; biofilm; micropreparative solution isoelectric focusing; high-performance liquid chromatography; capillary isoelectric focusing; sonicate from vascular catheter},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter},
   url = {http://www.sciencedirect.com/science/article/pii/S000326701301581X},
   volume = {812},
   year = {2014}
}

TY  - JOUR
ID  - 1138634
AU  - Vykydalová, Marie - Horká, Marie - Růžička, Filip - Duša, Filip - Moravcová, Dana - Kahle, Vladislav - Šlais, Karel
PY  - 2014
TI  - Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter
JF  - Analytica Chimica Acta
VL  - 812
IS  - FEB
SP  - 243-249
EP  - 243-249
PB  - ELSEVIER SCIENCE BV
SN  - 00032670
KW  - Candida parapsilosis group
KW  - biofilm
KW  - micropreparative solution isoelectric focusing
KW  - high-performance liquid chromatography
KW  - capillary isoelectric focusing
KW  - sonicate from vascular catheter
UR  - http://www.sciencedirect.com/science/article/pii/S000326701301581X
L2  - http://www.sciencedirect.com/science/article/pii/S000326701301581X
N2  - This study utilizes the high-performance liquid chromatography technique in combination with the new micropreparative solution isoelectric focusing fractionation on non-woven fabric strip for the characterization and differentiation of biofilm-positive and biofilm-negative forms of Candida parapsilosis sensu stricto on the basis of the changes in the composition of their cell-surface. Treatment of yeasts by boiling in distilled water relased surface substances from yeasts cells. Consequently, the optimized procedure has been used for fast identification of the highly pathogenic biofilm-positive Candida parapsilosis group in real clinical material – sonicate from vascular catheters. Moreover, the capillary isoelectric focusing was used as supporting and control technique. Obtained results suggest that this new method can be used to distinguish between biofilm-positive and negative forms of Candida parapsilosis sensu stricto.
ER  -

VYKYDALOVÁ, Marie; Marie HORKÁ; Filip RŮŽIČKA; Filip DUŠA; Dana MORAVCOVÁ; Vladislav KAHLE a Karel ŠLAIS. Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter. \textit{Analytica Chimica Acta}. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč.~812, FEB, s.~243-249. ISSN~0003-2670. Dostupné z: https://doi.org/10.1016/j.aca.2013.12.032.

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