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@article{1138867, author = {Štěpánková, Veronika and Damborský, Jiří and Chaloupková, Radka}, article_number = {6}, doi = {http://dx.doi.org/10.1002/biot.201200378}, keywords = {Haloalkane Dehalogenases}, language = {eng}, issn = {1860-6768}, journal = {Biotechnology Journal}, title = {Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases}, volume = {8}, year = {2013} }
TY - JOUR ID - 1138867 AU - Štěpánková, Veronika - Damborský, Jiří - Chaloupková, Radka PY - 2013 TI - Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases JF - Biotechnology Journal VL - 8 IS - 6 SP - 719-729 EP - 719-729 SN - 18606768 KW - Haloalkane Dehalogenases N2 - Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activity was induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed. Ethylene glycol and 1,4-dioxane were shown to have the most positive impact on the enantioselectivity. The favourable influence of the co-solvents on both activity and enantioselectivity makes DbjA suitable for biocatalytic applications. This study represents the first investigation of the effects of organic co-solvents on the catalytic performance of haloalkane dehalogenases and will pave the way for their broader use in industrial processes. ER -
ŠTĚPÁNKOVÁ, Veronika, Jiří DAMBORSKÝ a Radka CHALOUPKOVÁ. Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases. \textit{Biotechnology Journal}. 2013, roč.~8, č.~6, s.~719-729. ISSN~1860-6768. Dostupné z: https://dx.doi.org/10.1002/biot.201200378.
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