| KOUDELÁKOVÁ, Táňa, Radka CHALOUPKOVÁ, Jan BREZOVSKÝ, Zbyněk PROKOP, Eva ŠEBESTOVÁ, M. HESSELER, M. KHABIRI, M. PLEVAKA, D. KULIK, I. KUTA SMATANOVA, P. REZACOVA, R. ETTRICH, U.T. BORNSCHEUER a Jiří DAMBORSKÝ. Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel. Online. Angewandte Chemie International Edition. Verlag Chemie, 2013, roč. 52, č. 7, s. 1959-1963. ISSN 1433-7851. Dostupné z: https://dx.doi.org/10.1002/anie.201206708. [citováno 2024-04-23] |
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@article{1138880,
author = {Koudeláková, Táňa and Chaloupková, Radka and Brezovský, Jan and Prokop, Zbyněk and Šebestová, Eva and Hesseler, M. and Khabiri, M. and Plevaka, M. and Kulik, D. and Kuta Smatanova, I. and Rezacova, P. and Ettrich, R. and Bornscheuer, U.T. and Damborský, Jiří},
article_number = {7},
doi = {http://dx.doi.org/10.1002/anie.201206708},
keywords = {haloalkan dehalogenases},
language = {eng},
issn = {1433-7851},
journal = {Angewandte Chemie International Edition},
title = {Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel},
volume = {52},
year = {2013}
}
TY - JOUR
ID - 1138880
AU - Koudeláková, Táňa - Chaloupková, Radka - Brezovský, Jan - Prokop, Zbyněk - Šebestová, Eva - Hesseler, M. - Khabiri, M. - Plevaka, M. - Kulik, D. - Kuta Smatanova, I. - Rezacova, P. - Ettrich, R. - Bornscheuer, U.T. - Damborský, Jiří
PY - 2013
TI - Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel
JF - Angewandte Chemie International Edition
VL - 52
IS - 7
SP - 1959-1963
EP - 1959-1963
PB - Verlag Chemie
SN - 14337851
KW - haloalkan dehalogenases
N2 - Mutations targeting as few as four residues lining the access tunnel extended enzyme’s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 Grades C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.
ER -
KOUDELÁKOVÁ, Táňa, Radka CHALOUPKOVÁ, Jan BREZOVSKÝ, Zbyněk PROKOP, Eva ŠEBESTOVÁ, M. HESSELER, M. KHABIRI, M. PLEVAKA, D. KULIK, I. KUTA SMATANOVA, P. REZACOVA, R. ETTRICH, U.T. BORNSCHEUER a Jiří DAMBORSKÝ. Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel. Online. \textit{Angewandte Chemie International Edition}. Verlag Chemie, 2013, roč.~52, č.~7, s.~1959-1963. ISSN~1433-7851. Dostupné z: https://dx.doi.org/10.1002/anie.201206708. [citováno 2024-04-23]
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