Revised relative potency values for PCDDs, PCDFs, and
non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro
bioassay

J 2013

Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay

LEE, K.T.; S. HONG; J.S. LEE; K.H. CHUNG; Klára HILSCHEROVÁ et al.

Základní údaje

Originální název

Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay

Autoři

LEE, K.T.; S. HONG; J.S. LEE; K.H. CHUNG; Klára HILSCHEROVÁ; J.P. GIESY a J.S. KHIM

Vydání

Environmental Science and Pollution Research, Landsberg, Germany, Ecomed, 2013, 0944-1344

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.757

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/13:00071147

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1007/s11356-013-1770-2

UT WoS

000327498600029

Klíčová slova anglicky

Dioxin-like compounds; AhR activity; In vitro bioassay; Toxic equivalency factor; Relative potency

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2014 12:40, Ing. Andrea Mikešková

Anotace

V originále

While the World Health Organization 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalency factors are useful estimates of relative potencies of mixtures when conducting risk assessments, they are not useful when comparing the results of bioassays such as the H4IIE-luc to concentrations of TCDD equivalents calculated from instrumental analyses. Since there are thousands of dioxin-like compounds (DLCs), one use of screening assays is to determine if all of the aryl hydrocarbon receptor (AhR) active DLCs in a mixture have been accounted for in instrumental analyses. For this purpose, bioassay-specific relative potency (ReP) values are needed. RePs of 21 polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls that exhibit effects mediated through the AhR were determined by use of the H4IIE-luc assay. Different values of RePs are derived, depending on the statistical, curve-fitting methods used to derive them from the dose-response relationships. Here, we discuss the various methods for deriving RePs from in vitro data and their assumptions and effects on values of RePs. Full dose-response curves of 2,3,7,8-TCDD and other representative DLCs were used to estimate effective concentrations at multiple points (e.g., EC20-50-80), which were then used to estimate ReP of each DLC to 2,3,7,8-TCDD.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

Citovat

LEE, K.T.; S. HONG; J.S. LEE; K.H. CHUNG; Klára HILSCHEROVÁ; J.P. GIESY a J.S. KHIM. Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay. Environmental Science and Pollution Research. Landsberg, Germany: Ecomed, 2013, roč. 20, č. 12, s. 8590-8599. ISSN 0944-1344. Dostupné z: https://doi.org/10.1007/s11356-013-1770-2.

@article{1159646,
   author = {Lee, K.T. and Hong, S. and Lee, J.S. and Chung, K.H. and Hilscherová, Klára and Giesy, J.P. and Khim, J.S.},
   article_location = {Landsberg, Germany},
   article_number = {12},
   doi = {https://doi.org/10.1007/s11356-013-1770-2},
   keywords = {Dioxin-like compounds; AhR activity; In vitro bioassay; Toxic equivalency factor; Relative potency},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay},
   volume = {20},
   year = {2013}
}

TY  - JOUR
ID  - 1159646
AU  - Lee, K.T. - Hong, S. - Lee, J.S. - Chung, K.H. - Hilscherová, Klára - Giesy, J.P. - Khim, J.S.
PY  - 2013
TI  - Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay
JF  - Environmental Science and Pollution Research
VL  - 20
IS  - 12
SP  - 8590-8599
EP  - 8590-8599
PB  - Ecomed
SN  - 09441344
KW  - Dioxin-like compounds
KW  - AhR activity
KW  - In vitro bioassay
KW  - Toxic equivalency factor
KW  - Relative potency
N2  - While the World Health Organization 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalency factors are useful estimates of relative potencies of mixtures when conducting risk assessments, they are not useful when comparing the results of bioassays such as the H4IIE-luc to concentrations of TCDD equivalents calculated from instrumental analyses. Since there are thousands of dioxin-like compounds (DLCs), one use of screening assays is to determine if all of the aryl hydrocarbon receptor (AhR) active DLCs in a mixture have been accounted for in instrumental analyses. For this purpose, bioassay-specific relative potency (ReP) values are needed. RePs of 21 polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls that exhibit effects mediated through the AhR were determined by use of the H4IIE-luc assay. Different values of RePs are derived, depending on the statistical, curve-fitting methods used to derive them from the dose-response relationships. Here, we discuss the various methods for deriving RePs from in vitro data and their assumptions and effects on values of RePs. Full dose-response curves of 2,3,7,8-TCDD and other representative DLCs were used to estimate effective concentrations at multiple points (e.g., EC20-50-80), which were then used to estimate ReP of each DLC to 2,3,7,8-TCDD.
ER  -

LEE, K.T.; S. HONG; J.S. LEE; K.H. CHUNG; Klára HILSCHEROVÁ; J.P. GIESY a J.S. KHIM. Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay. \textit{Environmental Science and Pollution Research}. Landsberg, Germany: Ecomed, 2013, roč.~20, č.~12, s.~8590-8599. ISSN~0944-1344. Dostupné z: https://doi.org/10.1007/s11356-013-1770-2.

Přehled o publikaci