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@article{1159646,
author = {Lee, K.T. and Hong, S. and Lee, J.S. and Chung, K.H. and Hilscherová, Klára and Giesy, J.P. and Khim, J.S.},
article_location = {Landsberg, Germany},
article_number = {12},
doi = {http://dx.doi.org/10.1007/s11356-013-1770-2},
keywords = {Dioxin-like compounds; AhR activity; In vitro bioassay; Toxic equivalency factor; Relative potency},
language = {eng},
issn = {0944-1344},
journal = {Environmental Science and Pollution Research},
title = {Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay},
volume = {20},
year = {2013}
}
TY - JOUR
ID - 1159646
AU - Lee, K.T. - Hong, S. - Lee, J.S. - Chung, K.H. - Hilscherová, Klára - Giesy, J.P. - Khim, J.S.
PY - 2013
TI - Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay
JF - Environmental Science and Pollution Research
VL - 20
IS - 12
SP - 8590-8599
EP - 8590-8599
PB - Ecomed
SN - 09441344
KW - Dioxin-like compounds
KW - AhR activity
KW - In vitro bioassay
KW - Toxic equivalency factor
KW - Relative potency
N2 - While the World Health Organization 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalency factors are useful estimates of relative potencies of mixtures when conducting risk assessments, they are not useful when comparing the results of bioassays such as the H4IIE-luc to concentrations of TCDD equivalents calculated from instrumental analyses. Since there are thousands of dioxin-like compounds (DLCs), one use of screening assays is to determine if all of the aryl hydrocarbon receptor (AhR) active DLCs in a mixture have been accounted for in instrumental analyses. For this purpose, bioassay-specific relative potency (ReP) values are needed. RePs of 21 polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls that exhibit effects mediated through the AhR were determined by use of the H4IIE-luc assay. Different values of RePs are derived, depending on the statistical, curve-fitting methods used to derive them from the dose-response relationships. Here, we discuss the various methods for deriving RePs from in vitro data and their assumptions and effects on values of RePs. Full dose-response curves of 2,3,7,8-TCDD and other representative DLCs were used to estimate effective concentrations at multiple points (e.g., EC20-50-80), which were then used to estimate ReP of each DLC to 2,3,7,8-TCDD.
ER -
LEE, K.T., S. HONG, J.S. LEE, K.H. CHUNG, Klára HILSCHEROVÁ, J.P. GIESY a J.S. KHIM. Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay. \textit{Environmental Science and Pollution Research}. Landsberg, Germany: Ecomed, 2013, roč.~20, č.~12, s.~8590-8599. ISSN~0944-1344. Dostupné z: https://dx.doi.org/10.1007/s11356-013-1770-2.
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