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@article{1161956, author = {Kejnovská, Iva and Vorlíčková, Michaela and Brázdová, Marie and Sagi, Janos}, article_location = {Hoboken}, article_number = {4}, doi = {http://dx.doi.org/10.1002/bip.22400}, keywords = {DNA concentration; circular dichroism; folding topology; quadruplex; thermal and thermodynamic stability}, language = {eng}, issn = {0006-3525}, journal = {Biopolymers}, title = {Stability of human telomere quadruplexes at high DNA concentrations}, url = {http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract}, volume = {101}, year = {2014} }
TY - JOUR ID - 1161956 AU - Kejnovská, Iva - Vorlíčková, Michaela - Brázdová, Marie - Sagi, Janos PY - 2014 TI - Stability of human telomere quadruplexes at high DNA concentrations JF - Biopolymers VL - 101 IS - 4 SP - 428-438 EP - 428-438 PB - WILEY-BLACKWELL SN - 00063525 KW - DNA concentration KW - circular dichroism KW - folding topology KW - quadruplex KW - thermal and thermodynamic stability UR - http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract L2 - http://onlinelibrary.wiley.com/doi/10.1002/bip.22400/abstract N2 - For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A different phenomenon was observed, and is presented in this article, when the crowder was the quadruplex itself. With DNA strand concentrations ranging from 3 mM to 9 mM, the thermostability did not change up to 2 mM, above which it increased, indicating that the unfolding quadruplex units were not monomolecular above 2 mM. The results are explained by self-association of the G-quadruplexes above this concentration. The deltaG(o) 37 values, evaluated only below 2 mM, did not become more negative, as with the non-DNA crowders, instead, slightly increased. Folding topology changed from antiparallel to hybrid above 2 mM, and then to parallel quadruplexes at high, 6-9 mM strand concentrations. In this range, the concentration of the DNA phosphate anions approached the concentration of the K(+) counterions used. Volume exclusion is assumed to promote the topological changes of quadruplexes toward the parallel, and the decreased screening of anions could affect their stability. ER -
KEJNOVSKÁ, Iva, Michaela VORLÍČKOVÁ, Marie BRÁZDOVÁ and Janos SAGI. Stability of human telomere quadruplexes at high DNA concentrations. \textit{Biopolymers}. Hoboken: WILEY-BLACKWELL, 2014, vol.~101, No~4, p.~428-438. ISSN~0006-3525. Available from: https://dx.doi.org/10.1002/bip.22400.
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